Nyoman Adi Suratma
Laboratorium Parasitologi Veteriner, Fakultas Kedokteran Hewan Universitas Udayana, Jl. PB. Sudirman, Sanglah, Denpasar, Bali; Indonesia, 80234

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MICROBIAL CONTAMINATION TEST NUMBER OF YEAST MOLD SIMPLICIA MARIGOLD FLOWERS AS STANDARD RAW MATERIAL FOR HERBAL MEDICINE Maulia Suciani; I Made Merdana; Samsuri; Nyoman Adi Suratma
Buletin Veteriner Udayana Bul. Vet. Udayana. June 2025 Vol. 17 No. 3
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2025.v17.i03.p15

Abstract

The marigold plant is widely cultivated for medicinal purposes, as an ornamental plant and natural food colouring. Marigold flowers (Tagetes erecta L.) contain secondary metabolites in the form of terpenoids, essential oils, phenols, flavonoids, carotenoids, and so on. Looking at its contents and pharmacological benefits, marigold flowers have the potential to be used as simplicia as a raw material for herbal medicine. This research aims to determine the value of Total Yeast and Mold Count (TYMC) test simplicia marigold flower (Tagetes erecta L.) as a raw material for medicinal preparations based on microbial contamination standards according to the General Standard Parameters of BPOM RI Regulation Number 32 of 2019 concerning Quality Requirements for Traditional Medicines. This research is a type of non-experimental observational research because there is no treatment of the research object with a research design in the form of descriptive-quantitative analysis. The level of microbial contamination is reviewed through the Yeast and Mold Count (TYMC) test. The research data obtained was quantitative data which was analyzed by counting the number of microbes that grew on Potato Dextrose Agar (PDA) media after incubation at the appropriate growth temperature. The Total Yeast and Mold Count is 1,4 x 102 CFU/gram. The research results showed that marigold flower simplicia met the microbial contamination requirements for yeast and Mold Count numbers (≤5x105). It can be concluded that the mimosa leaves simplicia sample can be processed into drug preparations. Further research is needed on pathogenic microbial contamination, as well as additional quality standardization tests on marigold (Tagetes erecta L.) simplicia.
ESCHERICHIA COLI AND SHIGELLA SP. INFECTIONS IN BROILER CHICKENS AT A CLOSED HOUSE FARM IN BATUNGSEL VILLAGE, TABANAN I Putu Gde Surya Nagara Ardiana; Hapsari Mahatmi; I Ketut Berata; Nyoman Adi Suratma; Tri Komala Sari
Buletin Veteriner Udayana Bul. Vet. Udayana. December 2024 Vol. 16 No. 6
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2024.v16.i06.p20

Abstract

Escherichia coli is a coliform bacterium naturally found in the intestines of mammals. However, pathogenic strains, such as Avian Pathogenic Escherichia coli (APEC), can cause systemic infections and bacteremia in poultry. Infections by Escherichia coli in broilers lead to economic losses due to decreased production and increased mortality. This case report was conducted under protocol number 1/N/24, using anamnesis, clinical signs, epidemiological data, anatomical pathology, and histopathology observations to diagnose the condition. A 28-day-old white broiler chicken was collected from a closed house farm in Batungsel Village, Pupuan District, Tabanan Regency. Observed signs included lethargy, reduced appetite, an enlarged reddish abdomen, and white diarrhea. After the chicken's death, a necropsy was performed, and organ samples were preserved in 10% Neutral Buffered Formalin (NBF). Samples of the brain, lungs, liver, heart, spleen, kidneys, intestines, bursa, and feces were analyzed in histopathology, bacteriology, and parasitology laboratories. Histopathological preparations were stained with hematoxylin and eosin for microscopic examination. Bacterial infection tests included culturing samples from the intestines, liver, lungs, and heart on general, selective-differential, and Blood Agar media, followed by primary and secondary tests. The presence of Escherichia coli and Shigella sp. was confirmed. Parasite examinations using the flotation method showed no worm eggs or coccidia. These findings confirmed that the chicken was infected with Escherichia coli and Shigella sp.To prevent such infections, maintaining clean and sanitized housing is essential. Strict biosecurity measures are crucial to prevent external bacterial contamination. With good management practices, broiler chicken health can be optimally maintained.
PREVALENCE OF GASTROINTESTINAL NEMATODE INFECTION IN PIGLETS ON PIG BREEDING FARMS IN GIANYAR DISTRICT I Komang Pridayasa; I Wayan Gorda; Nyoman Adi Suratma; I Putu Cahyadi Putra
Buletin Veteriner Udayana Bul. Vet. Udayana. June 2025 Vol. 17 No. 3
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2025.v17.i03.p19

Abstract

Nematodiosis in piglets can inhibit nutrient absorption, leading to decreased growth performance. This study aimed to determine the prevalence of gastrointestinal nematode infections in piglets during the suckling and weaning phases at pig breeding farms in Gianyar Regency. This was a cross-sectional study with a purposive sampling method. A total of 216 fecal samples from suckling and weaning phase piglets from seven subdistricts in Gianyar Regency were used in the study. Fecal samples were examined using the flotation concentration method with saturated salt (NaCl) to identify nematode worm eggs. Prevalence data were described descriptively, while the Chi-square test was used to determine differences in nematode infection of suckling and weaning phase piglets. The results showed that the prevalence of nematodes was 41.66% (90/216), consisting of Strongyl type (33.33%), Ascaris suum (14.81%), Trichuris suis (6.48%), and Strongyloides ransomi (1.38%). The prevalence of nematode infection in weaning pigs (49.61%, 64/129) was higher (p<0.05) than that in the suckling pigs (29.88%, 26/87). Nematode co-infection in piglets was also observed in this study. The prevalence of nematode infection in piglets is notably high, particularly during the weaning phase of life. Consequently, it is advisable to implement nematode eradication strategies before weaning and to repeat these measures post-weaning.
SALMONELLOSIS CASE IN BROILER CHICKENS AT A CLOSED-HOUSE FARM IN DARMASABA VILLAGE, ABIANSEMAL, BADUNG, BALI Dewa Ayu Sinthya Devi; I Gusti Ketut Suarjana; I Made Kardena; Nyoman Adi Suratma; Ida Bagus Kade Suardana
Buletin Veteriner Udayana Bul. Vet. Udayana. June 2025 Vol. 17 No. 3
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2025.v17.i03.p36

Abstract

Salmonellosis caused by Salmonella sp. is a septicemic bacterial disease that commonly occurs in chickens. A 14-day-old broiler chicken from a closed house in Darmasaba Village, Abiansemal Subdistrict, Badung Regency, Bali was found to show general signs and clinical symptoms of conjunctivitis, decreased appetite, feather loss and dullness, stunted growth and greenish-white feces. To confirm the definitive diagnosis, laboratory examination is necessary. In this case study anatomical pathology, hispathological preparations, and bacterial identification were used. The examination results showed Enchepalitis, Pneumonia hemorrhagic, Myocarditis fibrinous, Hepatitis hemorrhagic, Glomerulonephritis hemorrhagic, Proventriculitis necrotican, and Enteritis hemorrhagic. Bacteriology test examination found Salmonella Typhimurium from the heart, lungs, and liver. The case animal was concluded to have Salmonellosis caused by Salmonella Typhimurium bacteria. Salmonellosis control can be done by improving biosecurity and cage sanitation, conducting chicken health management, controlling animal and human traffic, and using antibiotics wisely in accordance with veterinary recommendations to minimize the transmission of Salmonella Typhimurium bacteria through feed, water, and the environment.