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All Journal Buletin Veteriner Udayana
Ni Ketut Suwiti
Laboratorium Histologi Veteriner, Fakultas Kedokteran Hewan Universitas Udayana, Jl. PB. Sudirman, Sanglah, Denpasar, Bali; Indonesia, 80234
Immunology & microbiology Public Health Veterinary

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SECONDARY ANTIBODY TITER RESPONSE IN PIGS FOLLOWING STREPTOCOCCUS SUIS VACCINATION WITH VARIOUS ADJUVANTS: DETECTION USING SUPERNATANT ANTIGEN-BASED ELISA Gede Gita Pratama; I Nengah Kerta Besung; Hapsari Mahatmi; I Gusti Ngurah Kade Mahardika; Ni Ketut Suwiti
Buletin Veteriner Udayana Bul. Vet. Udayana. June 2025 Vol. 17 No. 3
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2025.v17.i03.p09

Abstract

Streptococcus suis is a pathogenic bacterium that causes severe disease in pigs and poses a zoonotic risk to humans. Vaccination is one of the primary preventive strategies, and its effectiveness can be enhanced through the use of appropriate adjuvants. This study aimed to evaluate the secondary humoral immune response in pigs vaccinated with a locally derived S. suis vaccine formulated with two different adjuvants: Montanide ISA 201 VG and Montanide Gel-01. In addition, the study observed the dynamics of antibody titters over time post-vaccination. Twelve pigs were divided into three treatment groups: a control group (unvaccinated), a group vaccinated with Montanide ISA 201 VG, and a group vaccinated with Montanide Gel-01. Blood samples were collected weekly for five weeks following the booster vaccination, and antibody titers were measured using ELISA based on culture supernatant antigens. Data were analyzed using analysis of variance and linear regression. The results showed that both adjuvants significantly increased antibody titers compared to the control (p < 0.05), with Montanide Gel-01 producing the highest titer (0.718 ± 0.0397), followed by ISA 201 VG (0.703 ± 0.0320), and the control group (0.459 ± 0.0419). Furthermore, the timing of serum collection significantly influenced antibody titer levels (p < 0.005). This study concludes that the use of Montanide adjuvants, particularly Gel-01, is effective in enhancing the humoral immune response to S. suis vaccination. Further research is recommended to evaluate the effects of increasing antigen concentration for achieving a more robust immune response.
HISTOLOGICAL STRUCTURE OF LYMPHOCYTES, MONOCYTES IN DOGS WITH DERMATITIS PRE AND POST THERAPY Made Ananda Giri Tanaya; Ni Ketut Suwiti; I Nyoman Suartha; Sri Kayati Widyastuti; Ni Luh Eka Setiasih; I Made Kardena
Buletin Veteriner Udayana Bul. Vet. Udayana. June 2025 Vol. 17 No. 3
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2025.v17.i03.p22

Abstract

A study was conducted to analyse the histological structure of lymphocytes and monocytes in dogs with dermatitis before and after therapy. Dermatitis is skin inflammation that can be caused by bacterial infections, allergies, or environmental factors. This study used 20 dogs with dermatitis that were given a combination of antiparasitic, antibiotic, anti-inflammatory, and antihistamine therapy. Blood samples were taken before and after therapy to be analysed using Morphology of Edge Blood (MDT) staining to observe changes in the histological structure of lymphocyte and monocyte cells. The results showed: before therapy, lymphocytes and monocytes experienced more necrosis (P<0.05) histological structure changes such as pycnosis, karyorexis, and karyolysis. After therapy, there was a decrease in the number of cells that experienced necrosis in lymphocytes, from 8.45 ± 2.08% to 6.85 ± 1.75%. Whereas in monocytes, there was a decrease in the number (P<0.05) of necrotised cells at post- therapy, from 27.5±19.4% to 13.5±10.3%. The conclusion of this study is that the histological structure of lymphocytes and monocytes that experience necrosis is less than cells that do not necrose. The histological structure of the cells showed a lot more normal. The combination therapy used proved effective in improving the structure of lymphocytes and monocytes.
Co-Authors Gede Gita Pratama Hapsari Mahatmi I Gusti Ngurah Kade Mahardika I Made Kardena I Nengah Kerta Besung I Nyoman Suartha Made Ananda Giri Tanaya Ni Luh Eka Setiasih Sri Kayati Widyastuti