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All Journal Journal of Pharmaceutical and Sciences. Edubiotik : Jurnal Pendidikan, Biologi dan Terapan
Munawaroh , Rima
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Nursing Public Health

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Aktivitas Antibakteri Ekstrak Etanol dan Dekokta Daun Jambu Kristal (Psidium guajava L. Cultivar Kristal) Terhadap Bakteri Escherichia coli dan Shigella sonnei Wardhani, Agviolisa Kusuma; Munawaroh , Rima
Journal of Pharmaceutical and Sciences JPS Volume 9 Nomor 1 (2026)
Publisher : Fakultas Farmasi Universitas Tjut Nyak Dhien

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36490/journal-jps.com.v9i1.1253

Abstract

Crystal guava leaves (Psidium guajava L. Cultivar Kristal) are plants that have potential as antibacterial agents against bacteria that cause diarrhea because they belong to the Psidium guajava L. species, which is widely reported to contain bioactive compounds such as flavonoids, tannins, and alkaloids. This study was conducted to determine the antibacterial activity of crystal guava leaves and the group of compounds that act as antibacterial agents. The study design was experimental, using ethanol extracts and decoctions of crystal guava leaves made at concentrations of 40% w/v, 20% w/v, 10% w/v, and 5% w/v and tested on bacteria that cause diarrhea, namely Escherichia coli and Shigella sonnei, using the well diffusion method. Then, TLC-Bioautography was performed to detect the compound components. Statistical data analysis using SPSS began with normality, homogeneity, Kruskal-Wallis, and Mann-Whitney tests. The results showed that the 40% w/v concentration had the largest inhibition zone in both extracts. The compounds contained in the ethanol extract and crystal guava leaf decoction were flavonoids, alkaloids, and tannins. Meanwhile, the specific compound that acts as an antibacterial agent in ethanol extracts and crystal guava leaf decoction is the flavonoid group, specifically quercetin. The Kruskal-Wallis test with a p-value of 0,001 < 0,005 showed that there was a significant difference between concentrations. Further analysis using the Mann-Whitney test showed that the 40% concentration group was significantly different from the 5% concentration group.
Development of primers amplifying DNA barcoding genes matK and rbcL of Legundi (Vitex trifolia) Hartanto, Nadhifah Riski; Sari , Banuwati Kartika; Rahayu, Triasuti; Munawaroh , Rima; Fawwaz , Muammar; Purwono , Bambang; Zulperi, Dzarifah; Prayitno, Trio Ageng; Sidiq, Yasir
Edubiotik : Jurnal Pendidikan, Biologi dan Terapan Vol. 10 No. 02 (2025): Edubiotik : Jurnal Pendidikan, Biologi dan Terapan
Publisher : Biology Education Department, Universitas Insan Budi Utomo, Malang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33503/ebio.v10i02.1497

Abstract

Legundi (Vitex trifolia) is one of the crucial ethno-pharmacological plants. However, the genetic exploration of these plants in Indonesia remains limited. Moreover, a set of primers can be an initial important step to explore the genetics of V. trifolia as well as to molecularly identify the species of Legundi. This study aimed to develop the two pairs of primers of maturase-K (matK) and ribulose bisphosphate carboxylase large subunit (rbcL) genes. This type of research is exploratory research using a quantitative approach. The research sampling are the leaves of the V. trifolia species, which were collected from Makassar, Indonesia, and the purposive sampling method. The data were obtained through DNA barcoding, and the data were analyzed using bioinformatics analysis. This study found two pairs of developed primers, matK and rbcL, successfully amplified both matK and rbcL target genes of V. trifolia. The newly developed species-specific primers successfully amplified the matK and rbcL genes of V. trifolia, and sequence analysis revealed high similarity values in BLAST and BOLD databases ranging from 99.2 to 100%, with PCR amplification of the matK marker showing particularly high DNA concentration and specificity for species-level identification. This study supports the genetic exploration and identification of useful ethno-pharmacy plants, V. trifolia. The conclusion of the study shows that two primer pairs of maturase-K (matK) and ribulose bisphosphate carboxylase large subunit (rbcL) genes have been developed from V. trifolia plants. This study supports the genetic exploration and identification of ethnopharmaceutical plants of V. trifolia and the role of bioinformatics tools in molecular studies of medicinal plants.
Co-Authors Hartanto, Nadhifah Riski Muammar Fawwaz Purwono , Bambang Rahayu, Triasuti Sari , Banuwati Kartika Trio Ageng Prayitno, Trio Ageng Wardhani, Agviolisa Kusuma Yasir Sidiq Zulperi, Dzarifah