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All Journal Bioeksperimen: Jurnal Penelitian Biologi Jurnal Riset Biologi dan Aplikasinya
Zulperi, Dzarifah
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Environmental Science Immunology & microbiology Medicine & Pharmacology

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Characterization and Molecular Identification of Lipolytic-bacterial Isolates Forming Biofilm on Polyethylene Plastic Ariyanti, Olivia Puja; Agustina, Putri; Anif, Sofyan; Seno, Hernandito Aryo; Hardianto, Alden Ganendra Madhava Priya; Mandiri, Listy Hasti; Zulperi, Dzarifah; Jamil, Nazia; Rahayu, Triastuti; Sidiq, Yasir
Jurnal Riset Biologi dan Aplikasinya Vol. 7 No. 1 (2025)
Publisher : Universitas Negeri Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26740/jrba.v7n1.p57-65

Abstract

Polyethylene (PE) plastic is one of the most widely used for multiple purposes and leading the environmental problems. Lipolytic bacteria are promising agents to reduce the plastic waste. The dry weight of PE plastic was reduced by 33 % in the Winogradsky's column after 45 days of incubation. However, the lipolytic bacteria responsible for those reduction was unknown. This study aimed to characterize and identify the potential lipolytic bacterial isolates forming biofilm on polyethylene PE plastics. Samples of lipolytic bacterial isolates were screened on tributyrin selective media based on the formed clear zone. Moreover, the 16S rRNA genes of the two most potential lipolytic bacterial isolates were amplified. Then, the amplicons of the 16S rRNA gene were sequenced. This study found two potential lipolytic bacteria isolates, AB A-2 and AB M-3, which had the characteristics of round colonies, wavy edges, convex surfaces, and milky white color. The two isolates are gram-positive and have the shape of Coccobacillus cells. The molecular identification showed that AB A-2 isolate was Bacillus sp., while AB M-3 isolate was Bacillus amyloliquefaciens. This finding contributes to novel bacterial isolates that potentially overcoming the plastic waste problem.
Efficacy of Endophytic Bacteria Pantoea dispersa Strain DSM 30073 on The Growth of Ipomoea reptans Rahayu, Triastuti; Sidiq, Yasir; Anhari, Minhatul Ulya; Zulperi, Dzarifah
Bioeksperimen: Jurnal Penelitian Biologi Vol 10, No 2: September 2024
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v10i2.23183

Abstract

Pantoea dispersa strain DSM 30073 is an endophytic bacterium isolated from the Ambon banana plant. From in vitro tests, this strain shows characteristics such as PGPB (Plant Growth Promoting Bacteria) and can support the growth of Cavendish banana plants, but its effect on Ipomoea reptans is unknown. The purpose of this study was to determine the efficacy of endophytic bacteria Pantoea dispersa strain DSM 30073 against the growth of Ipomoea reptans. The research design used was a one-factor Complete Randomized Design (CRD), namely inoculation frequency (P): control (P0), one-time inoculation (P1), and two-time inoculation (P2). The inoculum was confirmed to be pure by gram staining and cultured on NB medium with a 24-hour shake at room temperature. After 24 hours, the cells were harvested, and a suspension was made by adding Ringer's solution until it reached an OD600 nm of 0.8 – 1. Seeds were sterilized using 70 % alcohol followed by 1 % NaOCl for 1 minute each, then washed using sterile aquadest 3 times.  Sterile seeds were inoculated with an inoculum suspension for 30 minutes (P1). After inoculation, seeds are planted on a sterile soil medium. Repeated inoculation is carried out on the 11th day (P2). The growth of Ipomoea reptans is observed: plant height (cm), number of leaves (strands), leaf stretch (cm), wet weight of the plant (g), root length (cm), and number of roots (total). After 30 days, data on plant height 16.1 cm (control) – 17.4 cm (inoculation), wet weight of plants 1.1 g (control) – 1.4 g (inoculation 2 times), number of leaves 7 strands (control) – 8 strands (inoculation 2 times), and leaf stretch 10.2 cm – 10.9 cm (inoculation 2 times). Inoculation of Pantoea dispersa strain DSM 30073 had no effect on plant height growth, wet weight, leaf number, and leaf stretch but was significantly different in root parameters. It can be concluded that the Pantoea dispersa was not seen to support the growth of Ipomoea reptans significantly until 30 days after inoculation.
Co-Authors Anhari, Minhatul Ulya Ariyanti, Olivia Puja Hardianto, Alden Ganendra Madhava Priya Jamil, Nazia Mandiri, Listy Hasti Putri Agustina Seno, Hernandito Aryo Sofyan Anif Triastuti Rahayu Yasir Sidiq