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All Journal Bioscientist : Jurnal Ilmiah Biologi
Maulana, Zakiy
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Immunology & microbiology Social Sciences

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Pengaruh Pemberian Nanopartikel TiO₂ pada Berbagai Konsentrasi terhadap Perubahan Morfologi dan Biomassa Kalus Ginseng Jawa (Talinum paniculatum) secara In Vitro Maulana, Zakiy; Noli, Zozy Aneloi; Suwirmen; Fajrisani, Syifa; Putri, Suci Indah
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 4 (2025): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i4.18160

Abstract

This study aims to analyze the effect of titanium dioxide (TiO₂) nanoparticle concentrations on the characteristics of Talinum paniculatum (Java ginseng) callus. The experiment was conducted using a completely randomized design with six TiO₂ concentration treatments (0, 1, 2, 3, 4, and 5 ppm) over a 14-day incubation period. The observed parameters included callus morphology (color and texture), fresh weight, and dry weight. Fresh and dry weight data were analyzed using one-way analysis of variance (ANOVA) followed by Duncan’s New Multiple Range Test (DNMRT), while callus color and texture were analyzed descriptively. The results showed that the addition of TiO₂ nanoparticles induced changes in callus color ranging from yellow to yellow-reddish, and the resulting callus exhibited a compact texture. TiO₂ nanoparticles up to a concentration of 5 ppm did not show negative effects on either fresh or dry callus weight. These findings indicate that TiO₂ nanoparticles at the tested concentrations play a role in modifying the visual characteristics of callus without affecting biomass accumulation. Therefore, TiO₂ nanoparticles have potential application in enhancing secondary metabolite production.
Co-Authors Fajrisani, Syifa Putri, Suci Indah Suwirmen Zozy Aneloi Noli