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All Journal Jurnal Hortikultura Indonesia (JHI) BIOTROPIA - The Southeast Asian Journal of Tropical Biology Jurnal Biologi Tropis AGRIC Bioeksperimen
Suwirmen
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Economics, Econometrics & Finance Immunology & microbiology Social Sciences Veterinary

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Priming Benih Cabai (Capsicum annum L.) dengan Ekstrak Tumbuhan Paku (Davallia denticulata) yang Diekstrak dengan Beberapa Jenis Pelarut Suwirmen; Noli, Zozy Aneloi; Emelta, Citra
Agric Vol. 37 No. 1 (2025)
Publisher : Fakultas Pertanian dan Bisnis, Universitas Kristen Satya Wacana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24246/agric.2025.v37.i1.p79-85

Abstract

Priming benih merupakan perlakuan pada benih sebelum tanam dengan hidrasi terkontrol yang mampu meningkatkan perkecambahan. Salah satu ekstrak ekstrak yang berpotensi untuk dimanfaatkan sebagai bahan priming adalah paku (Davallia denticulata). Penelitian ini bertujuan untuk mengetahui jenis pelarut ekstrak dan konsentrasi terhadap perkecambahan benih cabai (Capsicum annum L.). Metode yang digunakan pada penelitian ini adalah Rancangan Acak Lengkap Faktorial dua faktor yang terdiri dari 3 ulangan, dilaksanakan bulan Agustus 2024. Penelitian ini dilakukan di Laboratorium Teaching 4, Jurusan Biologi, Universitas Andalas. Perlakuan terdiri dari jenis pelarut, a1 (aquades), a2 (metanol), a3 (etanol), dan a4 (butanol). Faktor kedua konsentrasi ekstrak paku kaki tupai, b0 (0 ml/l), b1 (25 ml/l), b2 (50 ml/l), dan b3 (100 ml/l). Hasil penelitian menunjukkan bahwa priming dengan jenis pelarut berpengaruh terhadap daya kecambah, kecepatan tumbuh, indeks vigor, panjang akar, dan berat basah kecambah. Konsentrasi ekstrak paku berpengaruh terhadap daya kecambah, kecepatan tumbuh, indeks vigor, panjang akar dan berat kering kecambah.
Optimizing The Effects of Auxin and Cytokinin Combinations on Shoot and Root Induction of Dendrobium mussauense Noli, Zozy Aneloi; Nur, Fauziah; Suwirmen
Jurnal Biologi Tropis Vol. 24 No. 2 (2024): April - Juni
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v24i2.6637

Abstract

Dendrobium mussaense, an orchid species of high ornamental value, was subjected to experimental treatments involving the addition of combinations of auxin and cytokinin, namely 2,4-D (2,4-dichlorophenoxyacetic acid) with BAP (6-benzylaminopurine) and 2,4-D with TDZ (thidiazuron). The research aim was to evaluate their effects on the emergence time of shoots and roots. The method used an experiment design with seven treatments. Among the treatments, the most effective was 2,4-D at a concentration of 1 mg/L combined with 0.75 mg/L of BAP. This treatment resulted in the shortest time for both shoot and root emergence. These findings underscore the importance of optimizing hormone combinations for efficient shoot and root induction in D. mussaense, providing valuable insights for orchid propagation and cultivation practices.
Induksi Embriogenesis Somatik Dendrobium mussauense melalui Teknik Thin Cell Layer (TCL) dengan Penambahan 2,4-D Noli, Zozy Aneloi; Suwirmen; Dinya Khairani Aisa Tumanggor
Jurnal Hortikultura Indonesia Vol. 16 No. 3 (2025): Jurnal Hortikultura Indonesia (JHI)
Publisher : Indonesian Society for Horticulture / Department of Agronomy and Horticulture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jhi.16.3.159-165

Abstract

In vitro culture provides an effective strategy for conserving and mass propagating the endangered orchid Dendrobium mussauense. This study aimed to evaluate the effects of different concentrations of 2.4-D on somatic embryogenesis (SE) induction using transverse thin cell layer (TCL) and non-TCL techniques. A factorial Completely Randomized Design (CRD) was employed, involving two factors: culture technique (TCL and non-TCL) and 2.4-D concentrations (1, 2, 3, and 4 mg L-¹), with four replications for each treatment. Explants were stem segments from 12-month-old D. mussauense plantlets. The highest survival and SE formation rates were observed in non-TCL explants treated with 4 mg L⁻¹ 2.4-D, reaching 75% and 50%, respectively. The earliest shoot and root emergence occurred at 13–14 and 23–25 days, respectively, under the same treatment. SE development in non-TCL explants treated with 2 mg L⁻¹ 2.4-D progressed through globular, scutellar, and coleoptilar stages within two weeks. Overall, 2.4-D concentrations of 2 and 4 mg L-¹ were most effective for SE induction in D. mussauense using both TCL and non-TCL techniques. Keywords: auxin, explant, SE, root induction, shoot induction.
Design and validation of PIP gene primer for quantitative PCR in Capsicum annuum using in silico and experimental approaches Nurhafitri, Amanda; Suwirmen
Bioeksperimen: Jurnal Penelitian Biologi March 2026
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v12i1.16129

Abstract

Capsicum annuum L. is an economically important horticultural crop whose productivity is strongly affected by drought stress. Plasma Membrane Intrinsic Protein (PIP), a member of the aquaporin gene family involved in water transport and osmotic regulation, represents a key target for drought stress studies, requiring highly specific and efficient primers for accurate gene expression analysis using quantitative PCR (qPCR). This study aimed to design and validate PIP gene primers for qPCR in C. annuum using integrated in silico and experimental approaches. Primer design was performed using NCBI Primer-BLAST based on the CaPIP reference sequence (XM_016711608.2), followed by in silico evaluation of primer specificity and secondary structure using Primer-BLAST and OligoAnalyzer. Nine primer pairs were initially generated and evaluated based on primer length, %GC, Tm, self3’ complementarity and amplicon size. Secondary structure analysis revealed strong self-dimer formation in pair 8, whereas pair 2 showed weak secondary structure within acceptable ΔG threshold (-9 kcal/mol). Experimental validation was conducted throught gradient PCR to optimize annealing temperature, followed by agarose gel 2%. Primer 2_CaPIP produced specific and clear amplification, with an optimal annealing temperature of 57.3oC. This study provides a validated CaPIP primer set suitable for qPCR-based gene expression analysis in C. annuum, supporting future molecular studies on drought stress tolerance.
YEAST EXTRACT ELICITATION  ENHANCES METABOLITE PRODUCTION IN Alocasia cuprea CALLUS Zozy Aneloi Noli; Suwirmen; Lily Ismaini; Meza Rani Maudya
BIOTROPIA Vol. 33 No. 3 (2026): BIOTROPIA Vol. 33 No. 3 September 2026
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2026.33.3.2649

Abstract

ARTICLE HIGLIGHTSYeast extract boosts metabolitesEnhances bioactive yieldBest at moderate dose (150 mg/L)ABSTRACTAlocasia cuprea is a tropical ornamental plant species belonging to the family Araceae, widely recognized for its distinctive and aesthetically appealing foliage. Additionally,  it represents a promising source of bioactive metabolites. This study aimed to evaluate the potential of yeast extract elicitation for enhancing metabolite production in A. cuprea callus culture established through in vitro propagation. The methods used in this study included callus induction, elicitation treatments, and phytochemical analysis of A. cuprea callus. The experiment was conducted using a completely randomized design (CRD) consisting of five yeast extract concentrations (0, 50, 100, 150, and 200 mg/L) with six replications. The results revealed that elicitation treatment did not affect callus biomass. Morphologically, the callus exhibited a friable texture and changed in color from off-white to yellowish-brown following elicitation. However, elicitation increased both the yield and diversity of metabolites in A. cuprea callus cultures. The optimal yeast extract concentration for enhancing the accumulation of major metabolites in A. cuprea was 150 mg/L. At this concentration, the levels of n-hexadecanoic acid, 9,12-octadecadienoic acid (Z,Z)-, and squalene increased by 6.67%, 82.49%, and 291.60%, respectively, compared with control. Nevertheless, the accumulation patterns of individual compounds varied across treatments, indicating that each metabolite exhibits a specific biosynthetic response to variations in yeast extract concentration.
Co-Authors Dinya Khairani Aisa Tumanggor Emelta, Citra Lily Ismaini Meza Rani Maudya Nur, Fauziah Nurhafitri, Amanda Zozy Aneloi Noli