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All Journal Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Jurnal Hortikultura Indonesia (JHI) Jurnal Penelitian Pendidikan IPA (JPPIPA) Jurnal Biologi Tropis Bioscientist : Jurnal Ilmiah Biologi AGRIC
Suwirmen
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Economics, Econometrics & Finance Education Immunology & microbiology Materials Science & Nanotechnology Physics Social Sciences

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Priming Benih Cabai (Capsicum annum L.) dengan Ekstrak Tumbuhan Paku (Davallia denticulata) yang Diekstrak dengan Beberapa Jenis Pelarut Suwirmen; Noli, Zozy Aneloi; Emelta, Citra
Agric Vol. 37 No. 1 (2025)
Publisher : Fakultas Pertanian dan Bisnis, Universitas Kristen Satya Wacana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24246/agric.2025.v37.i1.p79-85

Abstract

Priming benih merupakan perlakuan pada benih sebelum tanam dengan hidrasi terkontrol yang mampu meningkatkan perkecambahan. Salah satu ekstrak ekstrak yang berpotensi untuk dimanfaatkan sebagai bahan priming adalah paku (Davallia denticulata). Penelitian ini bertujuan untuk mengetahui jenis pelarut ekstrak dan konsentrasi terhadap perkecambahan benih cabai (Capsicum annum L.). Metode yang digunakan pada penelitian ini adalah Rancangan Acak Lengkap Faktorial dua faktor yang terdiri dari 3 ulangan, dilaksanakan bulan Agustus 2024. Penelitian ini dilakukan di Laboratorium Teaching 4, Jurusan Biologi, Universitas Andalas. Perlakuan terdiri dari jenis pelarut, a1 (aquades), a2 (metanol), a3 (etanol), dan a4 (butanol). Faktor kedua konsentrasi ekstrak paku kaki tupai, b0 (0 ml/l), b1 (25 ml/l), b2 (50 ml/l), dan b3 (100 ml/l). Hasil penelitian menunjukkan bahwa priming dengan jenis pelarut berpengaruh terhadap daya kecambah, kecepatan tumbuh, indeks vigor, panjang akar, dan berat basah kecambah. Konsentrasi ekstrak paku berpengaruh terhadap daya kecambah, kecepatan tumbuh, indeks vigor, panjang akar dan berat kering kecambah.
Application of fern Davallia denticuluta L. extract using different solvents as biostimulants for Kopay chili (Capsicum annuum L.) production Suwirmen; Noli, Zozy Aneloi; Marta, Fepi Dwi; Chairul
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 53 No. 2 (2025): Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy)
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24831/jai.v53i2.62870

Abstract

Ferns are a potential source of biostimulant compounds due to their rich content of bioactive secondary metabolites. This study aimed to evaluate different solvents for extracting bioactive compounds from Davallia denticulata and to identify the optimal extract formulation for enhancing the growth and yield of Kopay chili. This research was conducted from February to May 2024 at the Plant Physiology Research Laboratory and Greenhouse, Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Andalas, Padang. A completely randomized design with 16 treatment combinations involving four solvents (A1 = aquadest, A2 = methanol, A3 = ethanol, A4 = butanol) and four extract concentrations (B0 = 0 mg L-1, B1 = 25 mg L-1, B2 = 50 mg L-1, and B3 = 100 mg L-1) was employed. Plant growth parameters responded differently to various combinations of extract concentration and solvent type. Methanol extract at 100 mg L-1 significantly increased plant height, ethanol at 50 mg L-1 improved shoot dry weight and root biomass, butanol at 100 mg L-1 increased chlorophyll b and total chlorophyll, and methanol at 25 mg L-1 resulted in higher fruit weight. These results indicate that Davallia denticulata extract, when properly formulated, holds strong potential as a natural biostimulant to improve growth and productivity in chili cultivation. Keywords: active compouns; chili growth; plant extract; secondary metabolites; solvents extraction
Optimizing The Effects of Auxin and Cytokinin Combinations on Shoot and Root Induction of Dendrobium mussauense Noli, Zozy Aneloi; Nur, Fauziah; Suwirmen
Jurnal Biologi Tropis Vol. 24 No. 2 (2024): April - Juni
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v24i2.6637

Abstract

Dendrobium mussaense, an orchid species of high ornamental value, was subjected to experimental treatments involving the addition of combinations of auxin and cytokinin, namely 2,4-D (2,4-dichlorophenoxyacetic acid) with BAP (6-benzylaminopurine) and 2,4-D with TDZ (thidiazuron). The research aim was to evaluate their effects on the emergence time of shoots and roots. The method used an experiment design with seven treatments. Among the treatments, the most effective was 2,4-D at a concentration of 1 mg/L combined with 0.75 mg/L of BAP. This treatment resulted in the shortest time for both shoot and root emergence. These findings underscore the importance of optimizing hormone combinations for efficient shoot and root induction in D. mussaense, providing valuable insights for orchid propagation and cultivation practices.
Optimalisasi Konsentrasi Thidiazuron untuk Peningkatan Pertumbuhan Protocorm In Vitro Anggrek Grammatophyllum stapeliiflorum Noli, Zozy Aneloi; Arli, Naura Muthiah; Suwirmen
Jurnal Penelitian Pendidikan IPA Vol 10 No 12 (2024): December
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i12.9067

Abstract

Cytokinins are growth regulators that play a crucial role in cell division and shoot growth. Thidiazuron, a potent cytokinin, was the focus of our research. The research aimed to determine the effect of several concentrations of Thidiazuron on the protocorm growth of Grammatophyllum stapeliiflorum and to identify the best concentration of Thidiazuron (mg/L) for the protocorm growth of G. stapeliflorum orchids in vitro. The research was conducted from January until March 2022 at the Plant Physiology Laboratory, Universitas Andalas, Padang. The research used a Completely Randomized Design (CRD) with 5 treatments and 5 replications. Several concentrations of Thidiazuron were added to MS media with various concentrations: 0 mg L-1 (A), 0.50 mg L-1 (B), 1 mg L-1  (C), 1.50 mg L-1(D), and 2 mg L-1  (E). The parameters in this research were the percentage of life explants, day of shoot appearance, number of shoots, shoot length, number of leaves, number of roots, and root length. The results showed that Thidiazuron significantly affected the protocorm growth of the G. stapeliiflorum orchid compared to the control treatment. Notably, 2 mg L-1  Thidiazuron emerged as the best concentration for all growth parameters of protocorm G. stapeliiflorum, a finding that could significantly impact our understanding of orchid cultivation and plant physiology.
Pengaruh Pemberian Nanopartikel TiO₂ pada Berbagai Konsentrasi terhadap Perubahan Morfologi dan Biomassa Kalus Ginseng Jawa (Talinum paniculatum) secara In Vitro Maulana, Zakiy; Noli, Zozy Aneloi; Suwirmen; Fajrisani, Syifa; Putri, Suci Indah
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 4 (2025): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i4.18160

Abstract

This study aims to analyze the effect of titanium dioxide (TiO₂) nanoparticle concentrations on the characteristics of Talinum paniculatum (Java ginseng) callus. The experiment was conducted using a completely randomized design with six TiO₂ concentration treatments (0, 1, 2, 3, 4, and 5 ppm) over a 14-day incubation period. The observed parameters included callus morphology (color and texture), fresh weight, and dry weight. Fresh and dry weight data were analyzed using one-way analysis of variance (ANOVA) followed by Duncan’s New Multiple Range Test (DNMRT), while callus color and texture were analyzed descriptively. The results showed that the addition of TiO₂ nanoparticles induced changes in callus color ranging from yellow to yellow-reddish, and the resulting callus exhibited a compact texture. TiO₂ nanoparticles up to a concentration of 5 ppm did not show negative effects on either fresh or dry callus weight. These findings indicate that TiO₂ nanoparticles at the tested concentrations play a role in modifying the visual characteristics of callus without affecting biomass accumulation. Therefore, TiO₂ nanoparticles have potential application in enhancing secondary metabolite production.
Induksi Embriogenesis Somatik Dendrobium mussauense melalui Teknik Thin Cell Layer (TCL) dengan Penambahan 2,4-D Noli, Zozy Aneloi; Suwirmen; Dinya Khairani Aisa Tumanggor
Jurnal Hortikultura Indonesia Vol. 16 No. 3 (2025): Jurnal Hortikultura Indonesia (JHI)
Publisher : Indonesian Society for Horticulture / Department of Agronomy and Horticulture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jhi.16.3.159-165

Abstract

In vitro culture provides an effective strategy for conserving and mass propagating the endangered orchid Dendrobium mussauense. This study aimed to evaluate the effects of different concentrations of 2.4-D on somatic embryogenesis (SE) induction using transverse thin cell layer (TCL) and non-TCL techniques. A factorial Completely Randomized Design (CRD) was employed, involving two factors: culture technique (TCL and non-TCL) and 2.4-D concentrations (1, 2, 3, and 4 mg L-¹), with four replications for each treatment. Explants were stem segments from 12-month-old D. mussauense plantlets. The highest survival and SE formation rates were observed in non-TCL explants treated with 4 mg L⁻¹ 2.4-D, reaching 75% and 50%, respectively. The earliest shoot and root emergence occurred at 13–14 and 23–25 days, respectively, under the same treatment. SE development in non-TCL explants treated with 2 mg L⁻¹ 2.4-D progressed through globular, scutellar, and coleoptilar stages within two weeks. Overall, 2.4-D concentrations of 2 and 4 mg L-¹ were most effective for SE induction in D. mussauense using both TCL and non-TCL techniques. Keywords: auxin, explant, SE, root induction, shoot induction.
Co-Authors Arli, Naura Muthiah Chairul Dinya Khairani Aisa Tumanggor Emelta, Citra Fajrisani, Syifa Marta, Fepi Dwi Maulana, Zakiy Nur, Fauziah Putri, Suci Indah Zozy Aneloi Noli