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Detection of quinolone antibiotic resistance genes in Escherichia coli isolated from dairy cattle feces Raihan, Muhammad Ammar; Elsharkawy, Sara; Indrawati, Agustin; Latif, Hadri
Current Biomedicine Vol. 4 No. 1 (2026): January
Publisher : School of Veterinary Medicine and Biomedical Sciences, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/currbiomed.4.1.1

Abstract

Background Livestock raised in densely populated areas can serve as reservoirs for bacteria such as Escherichia coli, which may harbor antibiotic resistance genes that threaten both animal and human health. Objective This study aimed to identify and characterize quinolone resistance genes in E. coli isolated from dairy cattle feces. Methods Fifteen E. coli isolates were obtained from 15 dairy farms located in Kebon Pedes, Bogor, West Java. Genotypic detection of quinolone resistance genes was conducted using DNA sequencing on the MinION platform. Results All E. coli isolates (100%) carried at least one quinolone resistance gene. Of these, ten isolates (67%) contained a single resistance gene, while five isolates (33%) possessed two genes. The qnrS1_1 gene was identified in all isolates and represented the predominant genotype, whereas the qnrVC4_1 gene was found in five isolates (33%), mostly co-occurring with qnrS1_1. Both genes are plasmid-mediated and categorized as plasmid-mediated quinolone resistance (PMQR) genes. Conclusion The detection of qnrS1_1 and qnrVC4_1 genes in E. coli isolated from dairy cattle feces indicates that livestock manure may act as a reservoir for quinolone resistance genes, contributing to their persistence and potential spread within farm environments.
Deteksi Gen Resistansi Antibiotik Golongan Tetrasiklin pada Escherichia coli yang Diisolasi dari Kotoran Sapi Perah di Kota Bogor Kusuma, Nurlita Indah; Elsharkawy, Sara; Tiuria, Risa; Latif, Hadri
Jurnal Veteriner dan Biomedis Vol. 4 No. 1 (2026): Maret
Publisher : Sekolah Kedokteran Hewan dan Biomedis

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jvetbiomed.4.1.21-27.

Abstract

Escherichia coli (E. coli) merupakan bakteri indikator resistansi antibiotik yang banyak teridentifikasi pada kotoran sapi perah dan dapat menjadi reservoir utama dalam penyebaran gen resistansi antibiotik dari peternakan ke lingkungan sekitar. Penelitian ini bertujuan mendeteksi dan menganalisis keberadaan gen resistansi antibiotik golongan tetrasiklin (tet) pada E. coli yang diisolasi dari kotoran sapi perah di Kota Bogor. Sebanyak 15 isolat E. coli dari 15 peternakan di Peternakan Sapi Perah Kebon Pedes, Kota Bogor yang telah dikonfirmasi pada penelitian sebelumnya, kemudian dilakukan deteksi gen tet menggunakan MinION Oxford Nanopore Technologies. Hasil pengujian menunjukkan 8 dari 15 (53,3%) isolat E. coli teridentifikasi membawa gen tet dengan pola distribusi satu hingga dua gen per isolat. Gen yang paling banyak ditemukan adalah tetB (20%), diikuti oleh tetA, tetM, dan tetO dengan persentase yang sama (7%).  Sebanyak 60% gen yang teridentifikasi berasal dari plasmid dan 40% lainnya berasal dari kromosom. Dominasi gen pada plasmid mengindikasikan tingginya potensi penyebaran gen resistansi ke lingkungan sekitar, yang berisiko terhadap kesehatan masyarakat.