Article Per Year (5 Year)
p-Index From 2021 - 2026
0.23
P-Index
This Author published in this journals
All Journal Acta Pharmaciae Indonesia : Acta Pharm Indo
Salim, Prayoga Drajat Pangestu
Unknown Affiliation
Medicine & Pharmacology

Published : 1 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 1 Documents
Search

 1 
Comparative solvent evaluation for protein extraction from Channa striata and development of a stable capsule formulation for nutritional supplementation Salim, Prayoga Drajat Pangestu; Karim, Dewi Damayanti Abdul; Aziz, Syaikhul
Acta Pharmaciae Indonesia Vol 13 No 1 (2025): Acta Pharmaciae Indonesia: Acta Pharm Indo
Publisher : Pharmacy Department, Faculty of Health Sciences, Jenderal Soedirman University, Purwokerto, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.api.2025.13.1.16152

Abstract

Background: Channa striata is a protein-rich freshwater fish with therapeutic potential, yet systematic optimization of extraction methods and stable formulation development remain limited. Objective: To compare protein extraction efficiency using three solvents (1% HCl, 0.9% NaCl, and distilled water) and develop optimized capsule formulations. Methods: Protein was extracted at 1:4 (w/v) ratio and quantified using the Lowry method at 595 nm. The method was verified for linearity (R = 0.998), accuracy (98-102%), and precision (RSD <2%). Extracts were formulated into capsules at 0, 300, 400, and 500 mg (F0-F3) and evaluated for quality parameters and 28-day stability at -4°C, 24°C, and 40°C. Data were analyzed using one-way ANOVA with Duncan's post-hoc test. Results: The 1% HCl yielded highest protein content (4.947 ± 0.071 mg/mL) and extraction efficiency (21.390%) compared to 0.9% NaCl and distilled water (p <0.05). All formulations met pharmaceutical standards. Notably, F3 maintained protein retention >95% across all storage conditions (97.1-99.3%), while F0-F2 failed this criterion. Conclusion: The 1% HCl demonstrated optimal extraction efficiency. F3 (500 mg) exhibited superior stability and dissolution characteristics, representing a promising candidate for C. striata-based nutritional supplements.
Co-Authors Damayanti Abdul Karim, Dewi Syaikhul Aziz