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Elisitasi Kultur Sel Temulawak (Curcuma xanthorrhiza Roxb) untuk Produksi Senyawa Aktif Xantorizol Elfahmi, Elfahmi; Aziz, Syaikhul; Dana, Akbar
Acta Pharmaceutica Indonesia Vol 39, No 1 & 2 (2014)
Publisher : School of Pharmacy Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (266.053 KB)

Temulawak (Curcuma xanthorrhiza Roxb.) merupakan salah satu tanaman asli Indonesia yang telah digunakan untuk tujuan pengobatan. Xantorizol, senyawa seskuiterpenoid dari temulawak, telah banyak diteliti aktivitasnya. Kandungan senyawa xantorizol dari tanaman ini sangat kecil dan waktu panen relatif lama. Untuk mengoptimalkan produksi xantorizol, teknik kultur jaringan tanaman dapat digunakan sebagai salah satu alternatif. Penelitian ini ditujukan untuk meningkatkan kadar xantorizol dari kultur suspensi sel temulawak menggunkan elisitor. Kultur kalus yang telah diinisiasi pada media padat diinduksi menjadi suspensi sel dengan media cair. Kultur suspensi sel yang berumur dua minggu dan dielisitasi dengan ekstrak ragi. Kultur dipanen pada minggu pertama dan kedua setelah perlakuan dan dikeringkan. Sampel kering diekstraksi dengan etil asetat dan dianalisis dengan KCKT. Hasil analisis menunjukkan bahwa kultur yang dielisitasi dengan ekstrak ragi 100 ppm dapat menstimulasi pembentukan xantorizol sebesar 0,186%.Kata kunci: Curcuma xanthorrhiza Roxb., ekstrak ragi, kultur suspensi sel, temulawak.AbstractTemulawak (Curcuma xanthorrhiza Roxb.) is the one of indigenous plants in Indonesia that has been used for medicinal purpose. Xanthorrhizol, a sesquiterpenoid compound from temulawak, was studied for various activities. Xantorrhizol content in this plant is very low and relatively have long time for harvest. For optimize the production of xanthorrhizol, tissue culture technique could be used as an alternative. The aim of this research was carried out by to enhance the production of xanthorrhizol from cell suspension cultures using elicitors. The initiated callus cultures from solid medium, was induced to suspension cell cultures in liquid medium. The suspension cell culture was grown for two weeks and elicited with yeast extract. The cultures were harvested on the first and second weeks after elicited. Dry sample was extracted by ethyl acetate as a solvent and analyzed by HPLC. The results showed for elicitated culture by yeast extract 100 ppm could stimulate production of xanthorrhizol by 0.186%.Keywords: Curcuma xanthorrhiza Roxb., yeast extract, cell suspension culture, temulawak.

The Inhibition of 15-Lipoxygenase by Blechnum orientale Leaves and its Glycoside-flavonoid Isolates: In Vitro and In Silico Studies Rissyelly Rissyelly; Syaikhul Aziz; Frangky Sangande; Agung Wibawa Mahatva Yodha; Krisyanti Budipramana; Elfahmi Elfahmi; Sukrasno Sukrasno
HAYATI Journal of Biosciences Vol. 29 No. 3 (2022): May 2022
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.29.3.353-359

Fern is one of the groups of primitive plants rich in secondary metabolites that are commonly used to treat various diseases, including antioxidant, anti-hyaluronidase, anti-inflammation, and respiratory disease but less investigated. Flavonoid is one of the secondary metabolites abundantly present in ferns. This study aims to isolate major compounds found in Blechnum orientale act as 15-lipoxygenase (15-LOX) inhibitors. Inhibition of lipoxygenase decrease the production of leukotriene that induces bronchoconstriction in asthma. Isoquercitrin (Quercetin-3-O-β-glucopyranoside) and trifolin (kaempferol-3-O-β-D-galactoside) have been successfully isolated from Blechnum orientale. Further in silico study was performed to explain the binding mode between flavonoid pyranoside or galactoside and flavonoid aglycone in the 15-LOX cavity and their amino acid residues interaction. Isoquercitrin binds with Ile663, Ile400, Leu408, Leu597, Ala404, and Arg403 in the 15-LOX cavity as a lipoxygenase inhibitor. Trifolin binds the same amino acids as isoquercetin with addition His366, Gln596, and Phe175. Both isoquercitrin and trifolin act as competitive inhibitors against lipoxygenase enzymes.

Antibacterial activity of ethanolic extract of leaves and bulbs of Crinum asiaticum L. against acne-inducing bacteria Azrifitria .; Syaikhul Aziz; Chairul .
Indonesian Journal of Pharmacy Vol 21 No 4, 2010
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

The antibacterial activity of ethanolic extract of leaves and bulbs of Crinum asiaticum L. was tested against Propionibacterium acnes, Staphylococcus aureus and Staphylococcus epidermidis, pathogenic bacteria that cause acne. Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) were determined by dilution methods. MIC and MBC of ethanol leaves extract were found for P. acnes (1.25 and 2.5 mg/mL), S. aureus (5 and 10 mg/mL) and S. epidermidis (2.5 and 5 mg/mL). While MIC and MBC of ethanol bulbs extract were found for P. acnes (7.5 and 15 mg/mL), S. aureus (7.5 and 15 mg/mL) and S. epidermidis (3.75 and 7.5 mg/mL). Further study conducted on the ethanol leaves extract against P. acnes to analyze cell leakage (nucleic acid and protein) by ultraviolet spectrophotometry, metal ion (K+ and Ca2+) by atomic absorption spectrometry, and observed alteration of the cell wall by scanning electron microscopy (SEM). The result showed that ethanol leaves extract could damage the cell wall and affect the permeability of cell membrane which marked by release of nucleic acid (absorbance 0.3307-0.4299), protein (absorbance 0.0616-0.101), ion K+ (8.167-15.757 mg/mL), ion Ca2+ (5.47-13.74 mg/L) from the cell and alter morphology of cell wall of P. acnes.Key words: Antibacterial, Crinum asiaticum L., Propionibacterium acnes

Elisitasi Kultur Sel Temulawak (Curcuma xanthorrhiza Roxb) untuk Produksi Senyawa Aktif Xantorizol Elfahmi Elfahmi; Syaikhul Aziz; Akbar Dana
Acta Pharmaceutica Indonesia Vol. 39 No. 1 & 2 (2014)
Publisher : School of Pharmacy Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar

Temulawak (Curcuma xanthorrhiza Roxb.) merupakan salah satu tanaman asli Indonesia yang telah digunakan untuk tujuan pengobatan. Xantorizol, senyawa seskuiterpenoid dari temulawak, telah banyak diteliti aktivitasnya. Kandungan senyawa xantorizol dari tanaman ini sangat kecil dan waktu panen relatif lama. Untuk mengoptimalkan produksi xantorizol, teknik kultur jaringan tanaman dapat digunakan sebagai salah satu alternatif. Penelitian ini ditujukan untuk meningkatkan kadar xantorizol dari kultur suspensi sel temulawak menggunkan elisitor. Kultur kalus yang telah diinisiasi pada media padat diinduksi menjadi suspensi sel dengan media cair. Kultur suspensi sel yang berumur dua minggu dan dielisitasi dengan ekstrak ragi. Kultur dipanen pada minggu pertama dan kedua setelah perlakuan dan dikeringkan. Sampel kering diekstraksi dengan etil asetat dan dianalisis dengan KCKT. Hasil analisis menunjukkan bahwa kultur yang dielisitasi dengan ekstrak ragi 100 ppm dapat menstimulasi pembentukan xantorizol sebesar 0,186%.Kata kunci: Curcuma xanthorrhiza Roxb., ekstrak ragi, kultur suspensi sel, temulawak.AbstractTemulawak (Curcuma xanthorrhiza Roxb.) is the one of indigenous plants in Indonesia that has been used for medicinal purpose. Xanthorrhizol, a sesquiterpenoid compound from temulawak, was studied for various activities. Xantorrhizol content in this plant is very low and relatively have long time for harvest. For optimize the production of xanthorrhizol, tissue culture technique could be used as an alternative. The aim of this research was carried out by to enhance the production of xanthorrhizol from cell suspension cultures using elicitors. The initiated callus cultures from solid medium, was induced to suspension cell cultures in liquid medium. The suspension cell culture was grown for two weeks and elicited with yeast extract. The cultures were harvested on the first and second weeks after elicited. Dry sample was extracted by ethyl acetate as a solvent and analyzed by HPLC. The results showed for elicitated culture by yeast extract 100 ppm could stimulate production of xanthorrhizol by 0.186%.Keywords: Curcuma xanthorrhiza Roxb., yeast extract, cell suspension culture, temulawak.

SKRINING FITOKIMIA DAN ANALISIS KROMATOGRAFI LAPIS TIPIS EKSTRAK KULIT PISANG KEPOK Isna Mulyani; Rizki Nisfi Ramdhini; Syaikhul Aziz
JFL : Jurnal Farmasi Lampung Vol. 10 No. 1 (2021): JFL : Jurnal Farmasi Lampung
Publisher : Program Studi Farmasi Universitas Tulang Bawang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37090/jfl.v10i1.495

Kepok banana peel is an organic waste that has potential to be reused. Several studies proofed that banana peels have antioxidant activity, antimicrobial, inhibit the formation of cholesterol crystals and gallstones, diuretic effect, and mutagenic effect. This study aims to identify secondary metabolites contained in kepok banana peels using qualitative test methods (phytochemical screening) and thin layer chromatography analysis. The results of the phytochemical screening of kepok banana peel indicated the presence of alkaloids, monoterpenes/sesquiterpenes, phenols/tannins, saponins,and quinones. Thin layer chromatographic profile of ethanol extract showed the presence of flavonoid, phenol, and quinone compounds.Keywords: Phytochemical, chromatography, banana peel

TELAAH FITOKIMIA KULIT KACANG TANAH (Arachis hypogaea, L). Rizki Nisfi Ramdhini; Isna Mulyani; Syaikhul Aziz
JFL : Jurnal Farmasi Lampung Vol. 10 No. 1 (2021): JFL : Jurnal Farmasi Lampung
Publisher : Program Studi Farmasi Universitas Tulang Bawang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37090/jfl.v10i1.498

Peanut peel are a waste product of the peanut processing industry with little commercial value. Some of studies have been conducted indicating peanut peel can be beneficial as a source for traditional medicinal products since it is also rich of antioxidants. The aim of this research was to identify the content of secondary metabolites on the peanut peel. The method used was maseration with 96% ethanol. Phytochemical screening and assaying were performed using thin layer chromatography (TLC) method. The results of TLC analysis showed that the secondary metabolites in peanut peel were positive for flavonoids, alkaloids, tannins and quinon. Keywords: Peanut peel, Phytochemical, Thin-Layer chromatography (TLC)

Potensi Kandungan Antioksidan dari Daun Rhizophora apiculata Mangrove Khas Lempasing, Pesisir Lampung Selatan Muhammad Yogi Saputra; Muhammad Raffi Zakaria; Debora Silalahi; Welldone Sartika; Hamdiyah Fuola Zeri Hasibuan; Rahmat Kurniawan; Arif Azhari; Sena Maulana; Syaikhul Aziz; Sukrasno Sukrasno
Stannum : Jurnal Sains dan Terapan Kimia Vol 4 No 2 (2022): Oktober 2022
Publisher : Department of Chemistry - Universitas Bangka Belitung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33019/jstk.v4i2.3631

The mangrove woodland is a distinct bush or brine habitat characterized by a coastal sedimentation environment in which fine sediment (often rich in organic matter) accumulates in areas protected from high energy waves. Mangrove forests thrive on the coasts of tropical and subtropical regions, including Indonesia. Mangrove forest is a complex ecosystem that has a high diversity of plants, microorganisms, and animals. One of them is the Oil Mangrove (R. apiculata) which grows well on the coast of Lampung. This plant is hard, rich in tannins, and dense, mainly used to make charcoal and firewood. This plant traditionally used to treat diarrhea and nausea. Mangrove plants are tolerant to high salt levels, this special trait is due to the presence of secondary metabolites produced in response to various environmental stresses. Flavonoid compounds, alkaloids, terpenoids and steroids are secondary metabolites produced by mangrove plants. Secondary metabolite compounds from mangroves have bioactivity such as antidiabetic, antimicrobial, antioxidant and anticancer. Exploration of secondary metabolites from mangroves, especially oil mangroves locally named as Bakau Minyak, which are widely grown in the Lempasing area, coastal Lampung for the development of medicinal compounds, has not been intensely reported. This investigation aims to study the potential phytochemicals profile of R. apiculata’s leaf as an antioxidant

Penambatan molekul senyawa turunan orizanol terhadap enzim 3-hydroxy-3-methyl-glutaryl-coenzyme A (HMG-CoA) reduktase Syaikhul Aziz; Nur Adliani; Sukrasno Sukrasno
Journal of Science and Applicative Technology Vol 4 No 1 (2020): Journal of Science and Applicative Technology June Chapter
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat (LPPM), Institut Teknologi Sumatera, Lampung Selatan, Lampung, Indonesia

Oryzanol has been reported to reduce serum total cholesterol (hypolipidemic agent) by inhibiting HMG-CoA reductase, an enzyme responsible for the metabolic pathway that produces cholesterol and isoprenoid. The purpose of this experiment is to determine the inhibition activity of oryzanol derivatives on HMG-CoA reductase by molecular docking. Four structure of oryzanol derivatives, Lanosteryl-ferulate, Brassicasteryl-ferulate, Lupeol-ferulate, and Cholesteryl-ferulate were used as ligands for molecular docking. The HMG-CoA reductase structure was obtained from protein data bank and the study was performed using AutoDock Tools as a molecular docking software. All oryzanol derivatives show binding affinity against HMG-CoA reductase. Lupeol-ferulate was predicted to be the best inhibitory activity against HMG-CoA reductase because of molecular docking.

Pemanfaatan Digital Marketing sebagai Alternatif Pemasaran Produk pada CV. Buah Asa Tani, Bangunrejo, Lampung Tengah Winda Septiani; Syaikhul Aziz; Tantri Liris Nareswari; Abdul Muhyi; Murni Sari; Firly Shalsha Billa; Meiriska Maharani Surya Nugroho; Cresentia Aurora Mizuki; Shindy Martha Rahayu
Jurnal Pengabdian Dharma Wacana Vol 4, No 2 (2023): Jurnal Pengabdian Dharma Wacana
Publisher : Yayasan Pendidikan dan Kebudayaan Dharma Wacana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37295/jpdw.v4i2.444

Internet telah banyak digunakan oleh semua kalangan masyarakat, termasuk salah satunya yaitu para pengusaha yang sudah berjalan bertahun-tahun. Akan tetapi, kurangnya wawasan para pengusaha menyebabkan kurangnya pemanfaatan internet secara optimal dalam mendukung proses bisnis untuk meningkatkan keuntungan yang diperoleh para pelaku usaha. Salah satu usaha yang saat ini belum menyentuh digital marketing adalah CV. Buah Asa Tani. Kondisi ini mendorong untuk pelaksanaan pengabdian kepada masyarakat dalam mengedukasi terkait pemanfaatan sistem pemasaran secara digital sebagai alternatif pemasaran produk. CV. Buah Asa Tani merupakan Industri pengolahan kelapa, sejak tahun 2009. Usaha pemanfaatan kelapa, yang dikelola CV. Buah Asa Tani, menjadi sektor yang sangat penting untuk memastikan penyerapan produk kelapa dari para petani. Namun, masih terdapat beberapa permasalahan yang dihadapi, seperti stabilitas produk, pemasaran yang masih konvensional, serta persaingan pasar yang tinggi sehingga membutuhkan diversifikasi produk serta limbah. Walaupun penjualan VCO tergolong tinggi, namun permintaan produk tidak selalu ada. Dari berbagai problema yang ada pada Mitra, kegiatan pengabdian masyarakat ini berfokus pada pemanfaatan digital marketing, untuk meningkatkan pemasaran produk olahan kelapa. Materi digital marketing yang disampaikan kepada karyawan CV. Buah Asa Tani telah tersampaikan dengan baik, hal ini dibuktikan dengan adanya peningkatan pengetahuan peserta sebesar 100% setelah pelatihan.

In silico screening of pomegranate (Punica granatum) and celery (Apium graveolens) metabolites for potential anti-type 2 diabetes activity Auli, Winni Nur; Aziz, Syaikhul; Karim, Dewi Damayanti Abdul; Evanggeulista, Arnanda; Jannah, Aryn Fatkhul; Mendrofa, Alexander Yoel Harazachi
Acta Pharmaciae Indonesia : Acta Pharm Indo Vol 11 No 2 (2023): Acta Pharmaciae Indonesia: Acta Pharm Indo
Publisher : Pharmacy Department, Faculty of Health Sciences, Jenderal Soedirman University, Purwokerto, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.api.2023.11.2.11704

Background: Alpha (α)-amylase is a popular pharmacological target for controlling postprandial blood glucose levels. The exploration of natural ingredients for drug development is particularly promising. Notably, pomegranate (Punica granatum) and celery (Apium graveolens) are rich in phenols and flavonoids, making them potential candidates for anti-type 2 diabetes treatments. Objective: This study aims to identify the most promising derivatives from pomegranate and celery using a combined metabolomic and in silico approach. Methods: The study began by identifying metabolites from the KnapSack database, selecting based on primary and secondary metabolites also selecting them based on their pharmacokinetic profile. The selected metabolites were then docked with alpha-amylase (PDB ID: 2QV4). Furthermore, the interactions were analyzed using Discovery Studio, and toxicity profiles were assessed in silico using ECOSAR and Toxtree software. Results: The analysis identified punicaflavone and 2-deoxybrassinolide as the compounds with the highest binding affinity, at -10.06 kcal/mol and -10.89 kcal/mol respectively, both surpassing acarbose’s -9.33 kcal/mol. These compounds interacted with 11 common residues in alpha-amylase, mirroring acarbose’s interactions. In silico toxicity analysis revealed that punicaflavone might pose risks to aquatic organisms but does not exhibit potential as a genotoxic or non-genotoxic carcinogen. Conversely, 2-deoxybrassinolide displayed moderate toxicity to aquatic organisms but was also free from genotoxic and non-genotoxic carcinogenic potential. Conclusion: Punicaflavone and 2-deoxybrassinolide emerged as the most promising compounds, demonstrating strong binding affinities and similar interaction patterns with α-amylase as acarbose. Although both compounds may pose risks to aquatic environments, they do not show potential as genotoxic or non-genotoxic carcinogens, supporting their further exploration as anti-diabetic agents.

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