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All Journal Jurnal Laboratorium Khatulistiwa International Journal of Health Science
Natasya Intan Ramadhani
Politeknik Aisyiyah Pontianak
Biochemistry, Genetics & Molecular Biology Education Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Public Health Social Sciences

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Identifikasi Bakteri Escherichia Coli Pada Ulkus Diabetikum Studi di Klink Kanazawa Pontianak Menggunakan PCR gen 16S rRNA Nurhidayatulloh, Ariffialdi; Ramadhani, Natasya Intan; Abidin, Khoirul Rista; Suwandi, Edy; Salsabila, Aisha; Widodo, Kaltri; Andriani, Lulu; Hamidah, Najwa; Wulandari, Tri
Jurnal Laboratorium Khatulistiwa Vol 9, No 1 (2025): November 2025
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v9i1.1976

Abstract

Diabetes mellitus can lead to complications such as ulcers that are highly susceptible to bacterial infection. Escherichia coli is one of the pathogenic bacteria commonly found in diabetic foot ulcers. Accurate detection of E. coli species is essential to support appropriate infection management, and one of the reliable methods is Polymerase Chain Reaction (PCR) targeting the 16S rRNA gene. This study employed a descriptive design with an accidental sampling technique. Samples were obtained from swab specimens of diabetic foot ulcer patients. The procedures included culture on Eosin Methylene Blue (EMB) agar, Gram staining, bacterial DNA isolation, PCR amplification of the 16S rRNA gene, electrophoresis for visualization of PCR products, and sequencing for species confirmation. Based on the results of the study involving 30 diabetic foot ulcer patients at Kanazawa Clinic Pontianak, culture analysis showed that 4 isolates were positive for Escherichia coli. Gram staining further confirmed the presence of E. coli by demonstrating its characteristic morphology. The PCR results visualized through electrophoresis showed that 4 samples (13.3%) were positive for Escherichia coli based on the 16S rRNA gene, indicated by the presence of DNA bands at approximately 1500 bp. Sequencing analysis further confirmed the isolates as Escherichia coli with 99% similarity. 
Increasing Junior High School Students' Awareness of the Importance of Routine Hemoglobin Level Health Examination Puspa Amalia; Natasya Intan Ramadhani; Nisa Julia Safarti
International Journal Of Health Science Vol. 5 No. 1 (2025): : International Journal of Health
Publisher : Lembaga Pengembangan Kinerja Dosen

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55606/ijhs.v5i1.5694

Abstract

Anemia is when a person has fewer red blood cells or lower levels of hemoglobin in their blood than what is normal for their age and gender. In healthy people, red blood cells carry hemoglobin, which helps move oxygen and other nutrients like vitamins and minerals to the brain and body. Normally, males have a hemoglobin level of 13. 5 grams per deciliter, and females have 12 grams per deciliter.This community service was done at SMPN 05 Sungai Kakap and aimed to teach students about the importance of checking hemoglobin levels.The activity used educational talks and hemoglobin testing with a digital device called Easy Touch GCHb.The counseling sessions showed that the students were interested and asked many questions.The hemoglobin test found that out of 24 students tested, 4 girls had levels below 12 g/dl, which means they had anemia.The method used—talks and testing—worked well.The results were then compared with body mass index (BMI), but there was no link between the two. More checks and ongoing care are needed to stop anemia from happening early.
Co-Authors Abidin, Khoirul Rista Andriani, Lulu Hamidah, Najwa Nisa Julia Safarti Nurhidayatulloh, Ariffialdi Puspa Amalia Salsabila, Aisha Suwandi, Edy Tri Wulandari Widodo, Kaltri