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All Journal Jurnal Laboratorium Khatulistiwa
Abidin, Khoirul Rista
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Biochemistry, Genetics & Molecular Biology Education Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology

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Identifikasi Bakteri Escherichia Coli Pada Ulkus Diabetikum Studi di Klink Kanazawa Pontianak Menggunakan PCR gen 16S rRNA Nurhidayatulloh, Ariffialdi; Ramadhani, Natasya Intan; Abidin, Khoirul Rista; Suwandi, Edy; Salsabila, Aisha; Widodo, Kaltri; Andriani, Lulu; Hamidah, Najwa; Wulandari, Tri
Jurnal Laboratorium Khatulistiwa Vol 9, No 1 (2025): November 2025
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v9i1.1976

Abstract

Diabetes mellitus can lead to complications such as ulcers that are highly susceptible to bacterial infection. Escherichia coli is one of the pathogenic bacteria commonly found in diabetic foot ulcers. Accurate detection of E. coli species is essential to support appropriate infection management, and one of the reliable methods is Polymerase Chain Reaction (PCR) targeting the 16S rRNA gene. This study employed a descriptive design with an accidental sampling technique. Samples were obtained from swab specimens of diabetic foot ulcer patients. The procedures included culture on Eosin Methylene Blue (EMB) agar, Gram staining, bacterial DNA isolation, PCR amplification of the 16S rRNA gene, electrophoresis for visualization of PCR products, and sequencing for species confirmation. Based on the results of the study involving 30 diabetic foot ulcer patients at Kanazawa Clinic Pontianak, culture analysis showed that 4 isolates were positive for Escherichia coli. Gram staining further confirmed the presence of E. coli by demonstrating its characteristic morphology. The PCR results visualized through electrophoresis showed that 4 samples (13.3%) were positive for Escherichia coli based on the 16S rRNA gene, indicated by the presence of DNA bands at approximately 1500 bp. Sequencing analysis further confirmed the isolates as Escherichia coli with 99% similarity. 
Co-Authors Andriani, Lulu Hamidah, Najwa Nurhidayatulloh, Ariffialdi Ramadhani, Natasya Intan Salsabila, Aisha Suwandi, Edy Tri Wulandari Widodo, Kaltri