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All Journal Jurnal Penelitian Pendidikan IPA (JPPIPA) Journal of Innovative and Creativity
Larasati Arum Utami
Universitas Negeri Medan
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Mathematics Physics Social Sciences Other

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Identification of Microbial Contamination in Street Food Around Universitas Negeri Medan Larasati Arum Utami; Idramsa Idramsa; Artika Rindiani; Najwa Liliana; Nita Maya Sari Pane; Santaro Marito Nadeak
Journal of Innovative and Creativity Vol. 5 No. 2 (2025)
Publisher : Fakultas Ilmu Pendidikan Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/joecy.v5i2.2647

Abstract

Street food remains a popular choice for students due to its affordability and convenience. Its rapid preparation and service contribute to its ongoing popularity. The university, including Universitas Negeri Medan, is often surrounded by numerous street vendors offering a diverse range of foods, predominantly made from meat and flour. However, the open exposure of these foods and insufficient sanitation practices pose a heightened risk of contamination by harmful microorganisms. This study examines bacterial contamination in processed meat-based street foods sold near Universitas Negeri Medan, with a focus on items such as meatballs, grilled meatballs, and batagor. Microbiological examination involved isolating bacteria using selective and differential culture media, particularly Eosin Methylene Blue Agar (EMBA). Subsequent identification was carried out using Gram staining to determine cell morphology and SIM (Sulfide Indole Motility) medium for motility assessment in the microbiology lab at Universitas Negeri Medan. Findings revealed contamination predominantly in grilled meatballs, with bacteria belonging to the Enterobacteriaceae family detected. These isolates were characterized as Gram-negative, rod-shaped, and motile microorganisms. The presence of such bacteria raises serious concerns about food safety. It emphasizes the need for improved hygienic practices and rigorous food safety control measures in street food vending areas around the university. Implementing these measures is essential to safeguard student health and maintain the standard of street food quality on campus.
Potential Microplastic-Degrading Bacteria from Mangrove Sediment in The Paluh Getah Area, Percut Sei Tuan District Ahmad Shafwan S. Pulungan; Larasati Arum Utami; Marlinda Nilan Sari Rangkuti; Ayu Putri Ningsih; Cindy Suci Muliandhira
Jurnal Penelitian Pendidikan IPA Vol 11 No 10 (2025): October
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v11i10.12647

Abstract

Microplastic pollution poses a severe threat to marine ecosystems due to its persistence and toxicity, necessitating innovative biodegradation strategies. This study explored the potential of bacteria isolated from mangrove sediments of the Paluh Getah mangrove forest, North Sumatra, to degrade common microplastics, including LDPE, HDPE, PET, PP, and PS. Ten bacterial isolates were obtained and screened for their ability to grow on microplastic-supplemented mineral salt medium over 20 days. Three isolates (BPM 5, BPM 9, and BPM 10) demonstrated robust growth, with biochemical tests confirming catalase activity critical for managing oxidative stress during plastic degradation. Molecular identification via 16S rRNA gene sequencing revealed the isolates as Enterobacter sp. and Brevibacillus parabrevis, both known for their biodegradation capacities. The findings underscore the diverse metabolic pathways these bacteria employ to utilize microplastics as carbon sources. This research highlights the promise of mangrove sediment bacteria as eco-friendly bioremediation agents to mitigate microplastic pollution in coastal environments. Further studies are required to optimize degradation conditions and elucidate enzymatic mechanisms to enhance practical applications.
Co-Authors Artika Rindiani Ayu Putri Ningsih Cindy Suci Muliandhira Idramsa Idramsa Marlinda Nilan Sari Rangkuti Najwa Liliana Nita Maya Sari Pane Pulungan, Ahmad Shafwan S Santaro Marito Nadeak