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All Journal Jurnal Penelitian Pendidikan IPA (JPPIPA) Bioscientist : Jurnal Ilmiah Biologi
Akram
Universitas Tadulako
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education Immunology & microbiology Materials Science & Nanotechnology Physics Social Sciences

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Pengembangan E-Modul Eco-Enyzme untuk Mahasiswa MK Botani Ekonomi Hortikultura Prodi Pendidikan Biologi UNTAD Lestari M. P. Alibasyah; Astija; Isnainar; Vita Indri Febriani; Akram; Dwi Setyorini
Jurnal Penelitian Pendidikan IPA Vol 11 No 11 (2025): November
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v11i11.12805

Abstract

Learning in the Economic Botany and Horticulture course is still dominated by lectures, resulting in limited student procedural skills and the absence of structured teaching materials. This study aims to develop and evaluate an eco-enzyme e-module to improve conceptual understanding, practical competence, and learning effectiveness. Using a Research and Development (R&D) approach with the ADDIE model, the study involved expert validation, a limited trial with one class, and pre-test/post-test assessment. Data was analyzed through descriptive validation scores, practicality ratings, and N-gain effectiveness. Results show that expert validation yielded very feasible scores for content (90%), media (88%), and instructional design (86%). The practicality test produced a very practical overall score of 87%, indicating high usability, readability, visual appeal, accessibility, and engagement. Implementation of the e-module increased students’ mean score from 62.4 (pre-test) to 91.2 (post-test), generating an N-gain of 0.77 categorized as high effectiveness. The e-module successfully integrates conceptual explanations, fermentation procedures, multimedia features, and project-based activities related to eco-enzyme production. In conclusion, the eco-enzyme e-module is valid, practical, and effective for supporting independent learning and enhancing students’ conceptual and procedural skills in accordance with the course learning outcomes.
Phylogenetic Investigation of Megapodius cumingii from Kabetan, Central Sulawesi, Based on the RDP1 Gene Akram; I Made Budiarsa; Yunita Messe
Bioscientist : Jurnal Ilmiah Biologi Vol. 14 No. 1 (2026): March
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v14i1.20024

Abstract

This study investigated the phylogenetic relationships of Megapodius cumingii using bioinformatic analyses. Blood samples were collected from the Kabetan Island population, followed by DNA extraction using the QIAamp DNA Blood Mini Kit, PCR amplification with Takara Ex Taq™ and RDP1.U1/RDP1.L1 primers, and sequencing with BigDye Terminator v3.1 ABI PRISM 3100. Chromatograms were converted into nucleotide sequences using BioEdit, and sequence similarity was assessed using NCBI BLAST. Multiple sequence alignment, model selection, nucleotide composition analysis, and phylogenetic reconstruction were performed in MEGA 12. The RDP1 gene of M. cumingii from Kabetan showed a consistent nucleotide composition with high GC content (55.27%) and high similarity to M. cumingii sequence KF834037.1, with substitutions dominated by transitions (A↔G, T↔C). Maximum likelihood analysis under the T92+G model (1.03) revealed three major clades, with clades 1 and 2 strongly supported (bootstrap = 100). The Kabetan population formed a monophyletic subclade with KF834037.1 (bootstrap = 86) and showed a very short branch length (~0.05 substitutions/site), indicating low intraspecific divergence, with Megapodius tenimberensis as the closest sister taxon. These findings support the utility of RDP1 for phylogenetic resolution within Megapodiidae and provide a preliminary basis for conservation planning and population connectivity assessment of M. cumingii in Wallacea.
Co-Authors Astija Dwi Setyorini I Made Budiarsa Isnainar Lestari M. P. Alibasyah Vita Indri Febriani Yunita Messe