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All Journal Journal of Experimental and Clinical Pharmacy (JECP)
Ariani, Luysi
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Biochemistry, Genetics & Molecular Biology Chemistry Dentistry Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Nursing Public Health

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Comparative Antioxidant Capacity of Seed and Fruit Ethanol Extracts of Bitter Melon (Momordica charantia L.) Using FRAP Assay Ariani, Luysi; Rasyid, Faradiba Abdul; Yuliana, Dewi; Rahmawati, Rahmawati; Muflihunna, Andi; Syarif, Rezki Amriati
Journal of Experimental and Clinical Pharmacy (JECP) Vol 6, No 1 (2026): February 2026
Publisher : Poltekkes Kemenkes Gorontalo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52365/jecp.v6i1.1628

Abstract

Momordica charantia L. (bitter melon) is traditionally consumed as both food and medicine in Indonesia. Its fruit and seeds contain bioactive compounds such as saponins, flavonoids, polyphenols, and unsaturated fatty acids with recognized antioxidant potential. This study compared the antioxidant activity of ethanol extracts from bitter melon seeds and fruit using the Ferric Reducing Antioxidant Power (FRAP) assay. Extraction was conducted by ultrasound-assisted extraction (UAE) with ethanol as solvent, and antioxidant activity was expressed as quercetin equivalents (mg QE/g extract). The seed extract showed an antioxidant value of 8.680 mg QE/g, while the fruit extract exhibited 8.626 mg QE/g. The statistical analysis showed that the p-value was greater than 0.05, indicating no significant difference between the two extracts. This suggests that both extracts exhibit comparable antioxidant activity. These results highlight that both seeds and fruit of M. charantia may serve as promising natural antioxidant sources for functional food and pharmaceutical applications.
Co-Authors Abdul Rasyid, Faradiba Andi Muflihunna Dewi Yuliana Rahmawati, Rahmawati Rezki Amriati Syarif, Rezki Amriati