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All Journal Biology, Medicine, & Natural Product Chemistry Diagnosis: Jurnal Ilmiah Kesehatan Barigas: Medical and Health Science Journal
Hanasia Hanasia
Universitas Palangka Raya
Biochemistry, Genetics & Molecular Biology Health Professions Medicine & Pharmacology Nursing Public Health

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siRNAs targeting icaD Gene of Staphylococcus aureus to Inhibit Biofilm Formation: Structural Analysis and Efficacy Dinda Ananda Sulistina; Rian Ka Praja; Margaretha Yayu Indah Anugerahny; Hanasia Hanasia; Ysrafil Ysrafil
Biology, Medicine, & Natural Product Chemistry Vol 14, No 2 (2025)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2025.142.921-926

Abstract

Antibiotic resistance in Staphylococcus aureus infections, especially those involving biofilm formation, is a global health issue. Biofilm protects bacteria from the immune system and antibiotic treatment, making them 10 to 1000 times more resistant. The icaD gene, part of the ica operon, is crucial for biofilm synthesis by enhancing the enzymes responsible for forming the biofilm matrix. The icaD gene sequence of Staphylococcus aureus was obtained from the GenBank NCBI database with the accession code CP140612.1, with a gene sequence length of 306 bp and employed several bioinformatics methods, including siDirect for designing and evaluating effective siRNA sequences to select the most promising candidates. Additionally, siRNA Scales, MaxExpect, Duplex Fold, and siPred were employed to analyze the siRNA sequence length, secondary structure, binding energy, and efficacy predictions of siRNAs targeting the icaD gene. The study found that out of 54 siRNA candidates, siRNA22, siRNA50, and siRNA25 achieved inhibition rates of 93.69%, 92.82%, and 92.52%, respectively. These results bioinformatically demonstrated their potential to suppress the expression of the icaD gene and highlight their promise as siRNA-based antibacterial therapies to combat biofilm-related infections. The designed siRNA computationally shows potential as an innovative therapy to combat biofilm infections caused by Staphylococcus aureus.
Development of siRNA-Based Therapeutic Strategy Targeting Virulence Factor lpfA of Zoonotic Escherichia coli O157:H7 Rian Ka Praja; Oktaviani Naulita Turnip; Hanasia Hanasia; Reny Rosalina
Biology, Medicine, & Natural Product Chemistry Vol 15, No 1 (2026)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2026.151.159-165

Abstract

This study aimed to identify potential small interfering RNA (siRNA) molecules capable of silencing the lpfA virulence gene of Escherichia coli O157:H7, a zoonotic pathogen responsible for severe gastrointestinal disease. The nucleotide sequence of lpfA from E. coli O157:H7 strain Sakai was analyzed, and candidate siRNAs were designed using multiple bioinformatics tools, including siPRED, siRNA Scales, MaxExpect, and DuplexFold. Top ten selected siRNA candidates demonstrated high predicted inhibitory activity, with silencing efficiencies ranging from 93.73% to 94.66%. Secondary structure predictions indicated stable folding without inhibitory intramolecular structures, while binding energy analysis showed strong siRNA–mRNA duplex stability, with the best candidate exhibiting -26.5 kcal/mol. These findings suggest that the identified siRNAs possess strong theoretical potential to suppress lpfA expression and may serve as candidates for future experimental validation. Overall, this study provides a foundational step toward developing siRNA-based therapeutic strategies targeting key virulence factors of E. coli O157:H7.
Peran ATLM Dalam Pengembangan Dan Pelaksanaan Teknik Diagnostik Helicobacter Pylori : Tinjauan Literatur Terhadap Pemeriksaan Mikrobiologi Dan Molekuler Angga Pratama Putra; Agnes Cecilia; Stepany Teguh; Hanasia Hanasia; Rezjita Asti Pratiwi; Kezia Dona Gracia; Yuwita Laudya Christy; Gilbert Pahlawan Jonathan Gultom
Diagnosis Jurnal Ilmiah Kesehatan Vol. 21 No. 1 (2026): Diagnosis: Jurnal Ilmiah Kesehatan
Publisher : STIKES Nani Hasanuddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35892/djik.v21i1.3038

Abstract

Helicobacter Pylori (H. pylori) adalah bakteri gram negatif berflagel yang ditemukan di saluran pencernaan dapat menyebabkan komplikasi serius bila tidak ditangani dengan cepat dan tepat. Deteksi H. pylori dilakukan di laboratorium dengan pemeriksaan gold standar yaitu kultur dimana pemeriksaan dilakukan oleh seorang Ahli Teknologi Laboratorium Medis (ATLM). Penelitian ini dilakukan dengan pendekatan Systematic literature review dari 205 literatur dengan 17 literatur yang memenuhi kriteria inklusi mengenai berbagai metode diagnostik H. pylori, baik invasif maupun non-invasif. Tinjauan ini juga menyoroti peran penting seorang ATLM dalam seluruh tahapan pemeriksaan, mulai dari pengelolaan sampel, hingga validasi hasil. Terdapat beberapa spesimen untuk dilakukan pemeriksaan H. pylori yaitu biopsi lambung, feses, saliva, jaringan lambung, dan biopsi paru. Teknik diagnostik H. pylori mencangkup Urea Breath Test (UBT), Stool Antigen Test (SAT), rt-PCR (real-time PCR), ELISA, Rapid Urease Test (RUT), histologi, dan kultur. Biomarker untuk diagnosis dan terapi infeksi H. pylori yaitu Protein HpaA, gen VacA dan gen CagA.
Isolasi dan Identifikasi Bakteri Asam Laktat dari Minuman Fermentasi Berbahan Campuran Jahe, Lemon, dan Madu Kelulut Serta Uji Potensi Antibakteri terhadap Streptococcus pneumoniae: Isolation and Identification of Lactic Acid Bacteria from a Fermented Beverage Made from a Mixture of Ginger, Lemon, and Kelulut Honey and Evaluation of Its Antibacterial Potential against Streptococcus pneumoniae Raymond David Hermawan Mandolang; Hanasia Hanasia; Indria Augustina
Barigas: Medical and Health Science Journal Vol. 2 No. 1 (2026): Barigas : Medical and Health Science Journal
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/barigas.v2i1.11958

Abstract

Streptococcus pneumoniae is one of the main bacterial causes of pneumonia and is associated with high morbidity and mortality. Fermentation of functional food ingredients containing ginger, lemon, and kelulut honey has the potential to provide LAB with antibacterial activity; however, the characteristics of LAB and their ability to inhibit S. pneumoniae from this fermentation have not been widely investigated. To identify the types of LAB from a fermented beverage made from a mixture of ginger, lemon, and kelulut honey and to determine the antibacterial activity of the isolated LAB against S. pneumoniae. This laboratory experimental study used spontaneous fermentation of a mixture of ginger, lemon, and kelulut honey at fermentation days 0, 1, and 2. LAB isolates were obtained through serial dilution and plating on MRSA medium, then characterized based on colony morphology, Gram staining, and biochemical tests. Antibacterial activity against S. pneumoniae was evaluated using the disk diffusion method on Mueller Hinton Agar. The highest LAB colony count was obtained on day 1 of fermentation, whereas day 0 showed a lower colony count and day 2 showed a subsequent decrease. The isolates consisted of Gram‑positive, non‑spore‑forming, non‑motile cocci on days 0 and 2, phenotypically consistent with the genus Pediococcus, and Gram‑positive, non‑spore‑forming, non‑motile rods on day 1, consistent with the genus Lactobacillus. Disk diffusion testing showed that LAB isolates from days 0, 1, and 2 did not produce inhibition zones against S. pneumoniae. Fermentation of ginger, lemon, and kelulut honey yields LAB candidates phenotypically presumed to belong to the genera Pediococcus and Lactobacillus, with a peak colony count on day 1, however, under the fermentation conditions and antibacterial test method used, antibacterial activity against S. pneumoniae was not detected. Keywords: antibacterial activity, lactic acid bacteria, ginger, lemon, kelulut honey, S. pneumoniae.
Isolasi Bakteri Asam Laktat dari Tape Gambut dan Potensinya dalam Menghambat Bakteri Shigella dysenteriae: Isolation of Lactic Acid Bacteria from Tape Gambut and Their Potential to Inhibit Shigella dysenteriae Ammar Faiz; Hanasia Hanasia; Septi Handayani
Barigas: Medical and Health Science Journal Vol. 2 No. 1 (2026): Barigas : Medical and Health Science Journal
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/barigas.v2i1.12056

Abstract

Tape gambut is a fermented product from South Kalimantan that has the potential to contain LAB as a probiotic candidate. LAB is known to produce antimicrobial compounds such as organic acids, hydrogen peroxide, and bacteriocins, which can inhibit intestinal pathogenic bacteria such as S. dysenteriae, the main cause of shigellosis. This study aims to isolate LAB from tape gambut and identify its antibacterial activity against S. dysenteriae. This laboratory experimental study was conducted by isolating lactic acid bacteria (LAB) from tape gambut using MRSA medium through serial dilution. Isolate identification was performed macroscopically, by Gram staining, and biochemical tests. Antibacterial testing against S. dysenteriae was performed using the disk diffusion method on MHA medium, incubated at 37°C for 24 hours, and the inhibition zone was measured using a caliper. Biochemical tests showed that all isolates had characteristics similar to LAB, all isolates were Gram-positive, catalase-negative, motility-negative, and had a fermentative pattern on TSIA. All isolates tested using the disk diffusion method did not form inhibition zones on MHA medium, thus showing no antibacterial activity against S. dysenteriae under these test conditions. Tape gamhut contains bacteria with LAB characteristics, but the isolates obtained did not show inhibitory activity against S. dysenteriae. Further research with fermentation optimization and molecular identification is needed to assess its probiotic potential.
Co-Authors Agnes Cecilia Ammar Faiz Angga Pratama Putra Dinda Ananda Sulistina Gilbert Pahlawan Jonathan Gultom Indria Augustina Kezia Dona Gracia Margaretha Yayu Indah Anugerahny Oktaviani Naulita Turnip Raymond David Hermawan Mandolang Reny Rosalina Rezjita Asti Pratiwi Rian Ka Praja Septi Handayani Stepany Teguh Ysrafil, Ysrafil Yuwita Laudya Christy