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All Journal Biology, Medicine, & Natural Product Chemistry Jurnal Sains dan Kesehatan
Margaretha Yayu Indah Anugerahny
Universitas Palangka Raya
Biochemistry, Genetics & Molecular Biology Health Professions Medicine & Pharmacology Public Health

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siRNAs targeting icaD Gene of Staphylococcus aureus to Inhibit Biofilm Formation: Structural Analysis and Efficacy Dinda Ananda Sulistina; Rian Ka Praja; Margaretha Yayu Indah Anugerahny; Hanasia Hanasia; Ysrafil Ysrafil
Biology, Medicine, & Natural Product Chemistry Vol 14, No 2 (2025)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2025.142.921-926

Abstract

Antibiotic resistance in Staphylococcus aureus infections, especially those involving biofilm formation, is a global health issue. Biofilm protects bacteria from the immune system and antibiotic treatment, making them 10 to 1000 times more resistant. The icaD gene, part of the ica operon, is crucial for biofilm synthesis by enhancing the enzymes responsible for forming the biofilm matrix. The icaD gene sequence of Staphylococcus aureus was obtained from the GenBank NCBI database with the accession code CP140612.1, with a gene sequence length of 306 bp and employed several bioinformatics methods, including siDirect for designing and evaluating effective siRNA sequences to select the most promising candidates. Additionally, siRNA Scales, MaxExpect, Duplex Fold, and siPred were employed to analyze the siRNA sequence length, secondary structure, binding energy, and efficacy predictions of siRNAs targeting the icaD gene. The study found that out of 54 siRNA candidates, siRNA22, siRNA50, and siRNA25 achieved inhibition rates of 93.69%, 92.82%, and 92.52%, respectively. These results bioinformatically demonstrated their potential to suppress the expression of the icaD gene and highlight their promise as siRNA-based antibacterial therapies to combat biofilm-related infections. The designed siRNA computationally shows potential as an innovative therapy to combat biofilm infections caused by Staphylococcus aureus.
Prevalensi Rinosinusitis Kronik di Poliklinik THT-KL RSUD dr. Doris Sylvanus Kota Palangka Raya Tahun 2023 dan 2024 Ariska Agustina; Margaretha Yayu Indah Anugerahny; Indria Augustina; Angeline Novia Toemon; Nuch Sabunga
Jurnal Sains dan Kesehatan Vol. 10 No. 1 (2026): Juni : Jurnal Sains dan Kesehatan (JUSIKA)
Publisher : Universitas Muhamadiyah Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.57214/jusika.v10i1.1138

Abstract

Chronic rhinosinusitis (CRS) is a long-term inflammatory condition affecting the nasal mucosa and paranasal sinuses, which significantly impacts patients’ quality of life due to persistent and recurrent symptoms. Limited epidemiological data regarding CRS are available in Central Kalimantan, particularly at RSUD dr. Doris Sylvanus in Palangka Raya, prompting this study to determine its prevalence and patient distribution characteristics. This research employed a quantitative descriptive design with a cross-sectional approach. Data were collected from medical records of patients diagnosed with CRS in 2023 and 2024. Univariate analysis was conducted, and results were presented in tables and diagrams illustrating frequency distributions by year, gender, age, and main complaints. The findings showed a total of 118 CRS patients, with 53 cases in 2023 and 65 cases in 2024. The prevalence of CRS increased from 4.91% in 2023 to 10.83% in 2024. Based on gender, males accounted for 50.8% (60 patients), while females represented 49.2% (58 patients). The age group most affected was 46–55 years (31%), indicating a predominance among individuals of productive age. Nasal congestion was the most frequent primary complaint, reported by 35% of patients. The study concludes that the prevalence of CRS at RSUD dr. Doris Sylvanus increased notably from 2023 to 2024, with a slightly higher incidence among males in the productive age group. These findings highlight the need for enhanced preventive measures and early diagnosis to manage CRS more effectively in the local population.
Co-Authors Angeline Novia Toemon Ariska Agustina Dinda Ananda Sulistina Hanasia Hanasia Indria Augustina Nuch Sabunga Rian Ka Praja Ysrafil, Ysrafil