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All Journal African Journal of Biochemistry and Molecular Biology Research African Journal of Sciences and Traditional Medicine
Ojochenemi E. Yakubu
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Biochemistry, Genetics & Molecular Biology Chemistry Health Professions Immunology & microbiology Nursing Public Health

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The Comparative Suitability of Different Solvents Used in the Fractionation of Methanol Leaf Extract of Persea americana Mill: An in Vitro Studies Ojochenemi E. Yakubu; Olawale Otitoju; Janya Danjuma; dulrashid Dauda Maianguwa; Isaac John Umaru; Gloria Omonefe Oladele
African Journal of Biochemistry and Molecular Biology Research Vol 1 No 1 (2024): African Journal of Biochemistry and Molecular Biology Research
Publisher : Darul Yasin Al Sys

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58578/ajbmbr.v1i1.3664

Abstract

This research investigated the suitability of various solvents used in the fractionation of methanol leaf extract of P. americana to ascertain the flavonoid, vitamin and amino acid profile of the fractions. It has been demonstrated that a number of Persea americana plant components contain different phytochemicals associated with important biological function. Fresh leaves of P. americana were harvested from Baissa in Taraba State. The plant materials were air dried under shade at ambient temperature. The dried plant materials were pulverized to fine powder using mortar and pestle. The pulverized plant material was soaked in methanol for 72 hours for extraction. The extract was filtered using clean filter cloth and filter paper under reduced pressure. The filtrate was evaporated in in rotary evaporator and water bath to obtain the required concentrate. The extract was used to perform fractionation using separation funnel and solvents of different polarity such n-hexane, methanol and ethanol. Each of the fractions was used to analyze for flavonoid, vitamin and amino acid profile using HPLC. The result reveals that quercetin is present in each of the fractions. Caffeic acid phenyl ether was detected in diluted ethanol fraction, coumaric acid was identified in diluted n-hexane fraction, caffeic acid was identified in methanol fraction chlorogenic acid detected in n-hexane fraction. Vitamin K was identified in all the fractions, Vitamin B1 was identified in diluted ethanol fraction and n-hexane fraction while vitamin B9 was detected in diluted n-hexane fraction and n-hexane fraction. Vitamins B2 and B6 were identified in methanol fraction and diluted ethanol fraction respectively. The diluted ethanol fraction was seen to contain 8 different amino acids followed by the methanol fraction with 6, then the diluted n-hexane fraction with 3 and the n-hexane fraction with 2. This reveals that for isolation of amino acids, the ethanol fraction may be more suitable considering that the highest number of amino acids were found in it.
Stembark Methanol Extract of P. americana: Total Antioxidant Capacity, Total Flavonoids Content, Total Phenolics Content and Its Flavonoids, Vitamins and Amino Acids Profile Ojochenemi E. Yakubu; Michael S. Abu; Janya Danjuma; Isaac John Umaru; Gloria Omonefe Oladele
African Journal of Sciences and Traditional Medicine Vol 1 No 1 (2024): African Journal of Sciences and Traditional Medicine
Publisher : Darul Yasin Al Sys

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58578/ajstm.v1i1.3677

Abstract

This research investigated the total antioxidant capacity, total flavonoids content, total phenolics content as well the flavonoids, vitamins and amino acids profile of stembark methanol extract of P. americana. Plant materials have been known be rich source of various medicinal active ingredients. These may include alkaloids, terpenoids, saponins, phenolic compounds, flavonoids, and tannins as well as nutritional components including sugars, amino acids as well as proteins in addition to other vital components. The stembark of P. americana was harvested in its plant in Baissa, Taraba State, Nigeria. The plant material was air dried and pulverized and soaked in methanol for 72 hours before filtration. The filtrate was further concentrated using rotary evaporator and water bath. The concentrate was analyzed for total antioxidant capacity using DPPH, flavonoids content using aluminium chloride and phenolics content using folin ciocaltue. Flavonoids, vitamins and amin acids profile was determined using HPLC. The result total antioxidant capacity shows that inhibition increased with concentration with the 100µL/100mL had inhibition percentage of 87.88% whereas the lowest concentration of 31.25µL/100mLhad the inhibition percentage of 40.14%. The results for total flavonoid content and total phenolics content show significant levels with 42.21±0.23mg QE/100g and 33.65±3.02mg GAE/100g respectively. The result for flavonoid and phenolics profile reveals that quercetin and caffeic acid phenyl ester were identified with 0.50% and 99.50% respectively. For vitamins, vitamin K was detected with 0.40%, vitamin B1 with 80.20% and vitamin B2 with 19.50% abundance. There seven different amino acids identified, these include asparagine, threonine, phenylalanine, aspartic acid, glutamic acid, leucine and arginine. The outcomes reveal that the plant material could possess important phytochemicals which could be used in disease treatment as well as vital macromolecules that can be used as supplements.
Co-Authors dulrashid Dauda Maianguwa Gloria Omonefe Oladele Isaac John Umaru Janya Danjuma Michael S. Abu Olawale Otitoju