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All Journal African Journal of Biochemistry and Molecular Biology Research African Journal of Clinical Medicine and Pharmacy Research
Iseko Iyoko Kingsly
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Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Nursing Public Health

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Effect of Administration of Copper plasma Activated water and Anesthetic on Hematological and Pancreatic tissues of Wister rats Isaac John Umaru; Iseko Iyoko Kingsly; Prince Kwadwo Gyasi; Edom Favour Ogochukwu; Tensaba Andes Akafa; Jackson Soyinka Malantso; Bilyaminu Habibu; Kerenhappuch Isaac Umaru
African Journal of Biochemistry and Molecular Biology Research Vol 2 No 1 (2025): African Journal of Biochemistry and Molecular Biology Research
Publisher : Darul Yasin Al Sys

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58578/ajbmbr.v2i1.4722

Abstract

In biomedicine, applications of PAW span from biofilm removal, wound healing, deactivation of bacteria and viruses, dentistry (for teeth disinfection and whitening), and cancer therapy. The biochemical activity of PAW is derived from synergistic effects of the highly reactive species, specifically reactive oxygen and nitrogen species (RONS). PAW is considered a biofriendly and prospective solution for biotechnology applications due to the time dependent nature of its biochemical activity because of the active species, and its economic and environmental benefits of using air rather than toxic chemicals as the raw material. Thus, the study on Effect of Administration of Copper plasma Activated water and Anaesthetic on Haematological and Pancreatic tissues of Wister rats. Material and Methods: Preparation of plasma-activated water a non-thermal micro-hollow cathode discharge (MHCD) was used to generate plasma-activated waters (CU-PAWs). Results: The results showed the haematological profile of the animals treated with 400mgkg-' had a decrease in Haemoglobin, Mean capsular Haemoglobin (11.21 ± 1.23 and 22.93 ± 21.11) compared to the controlled group (15.03 ± 1.72 and 29.93 ± 7.27) and in animals treated with 200mgkg-1 and 400mgkg-1 there is a significant decrease in the platelets (21.96 ± 23.16 and 20.89 ± 24.03) compared to animals on control group (32.08± 16.70) (table 2). Animals treated with 200mgkg-' and 400mgkg-' have a decrease in platelet (22.36 ± 4.88 and 20.89 ± 24.03) compared to animals on control (32.08 ± 16.70) and also animals treated with 400mgkg-1 have an elevated white blood cell count 173-56 ± 4.50) compared to the control group (3.66 ± 3.06). Conclusion: Cupper plasma water is safe to be used since it do not have much effect on the blood biochemistry and haematological parameters unlike the aesthetic agents cause changes on the haematological and blood biochemistry parameters. Hence, it is important to be aware of the effects of these agents before using them in experiments.
In-vitro Cytotoxic and Anti-vibro Cholerae Activities of Methanol Roots Extracts of Morinda citrifolia L (Noni) Isaac John Umaru; Daniel Konka; Iseko Iyoko Kingsly; Solomon Ossom Asare; Okrah George Hassan; Joseph Oteng; Tensaba Andes Akafa; Kerenhappuch Isaac Umaru
African Journal of Clinical Medicine and Pharmacy Research Vol 2 No 1 (2025): African Journal of Clinical Medicine and Pharmacy Research
Publisher : Darul Yasin Al Sys

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58578/ajcmpr.v2i1.4720

Abstract

Knowledge on medicinal properties of plants has extensively been applied all over the World to cure or ameliorate suffering of mankind. The use of remedies from plant origin covers a wide variety of maladies and constitutes an alternative way to antibiotics, which is otherwise no longer promising due to the generalized antibiotic resistance among pathogenic microorganisms. The majority of epidemic Vibrio cholerae strains have become resistant to multiple antimicrobial agents for many reasons, including mutations, horizontal gene transfers, etc. Therefore, secondary means of treatment are needed to overcome this impediment in combating cholera. Morinda citrifolia roots extensively used in traditional medicine. Therefore, the present study aims to evaluate the In-vitro Cytotoxic and Anti-vibro Cholerae Activities of Methanol Roots Extracts of Morinda citrifolia against V. cholerae isolates. Methods: Initially, different concentration of the extracts materials was subjected to qualitative phytochemical screening. Anti-V.cholerae activities were assessed by performing MIC (minimum inhibitory concentration) test using disc diffusion method with different concentrations (1.25, 2.25, 5.00, 8.00, 10.00mg/ml) where standard discs of gentamycin and meropenem (10 μg/disc) were used. Further, Brine shrimp lethality bioassay was done to evaluate the extracts’ cytotoxic activities using different concentrations (5,10, 20, 50, 100, 200, 400, 800μg/ml) against vincristine sulphate. Results: The preliminary screening confirms the presence of convenient phytochemical constituents like flavonoids, alkaloids, glycosides, tannins, etc., and are liable for the emergence of the antibacterial activities in different strains. The minimum inhibitory concentrations of methanolic Morinda citrifolia roots was found to be (a) 1.25, (b) 2.25, (c) 5, (d) 8, and (f) 10 mg/ml, respectively. Moreover, 100% mortality was observed only at the highest concentration (800 μg/ml). In comparison, the rest of the test concentration showed less than 90% mortality at the highest concentration (400 μg/ml). Conclusions: The Morinda citrifolia roots possess significant phytochemicals and showed potential anti-V. cholerae activities with mild toxicity. Hence, Morinda citrifolia (L.) roots may be recommended as potential phytotherapy against cholera disease.
Anticancer effect of Copper Activated Plasma Water on MCF7 Breast Cancer Cells Isaac John Umaru; Iseko Iyoko Kingsly; Edom Favour Ogochukwu; Tensaba Andes Akafa; Jackson Soyinka Malantso; Bilyaminu Habibu; Kerenhappuch Isaac Umaru
African Journal of Clinical Medicine and Pharmacy Research Vol 2 No 1 (2025): African Journal of Clinical Medicine and Pharmacy Research
Publisher : Darul Yasin Al Sys

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58578/ajcmpr.v2i1.4721

Abstract

Broad biological activities of “plasma-activated water” (PAW) have drawn great attentions recently. Treatment of water using gas discharge plasma led to acidic solutions with excellent and broad antibacterial activity. Because PAW caused severe membrane damages in bacteria and diffused freely in extracellular matrix, PAW also demonstrated good anti-biofilm activity. However, further studies revealed that trace amounts of metal ions (mainly copper) in PAW brought by plasma treatment played key roles in bacteria inactivation. The contribution of metal ions to the antibacterial activity varied among PAWs from different working gases. However, solution acidification caused by reactive species in plasma was essential. The experimental results demonstrated that potential artifacts in reported biological activities of PAWs should be considered. Therefore, Copper has important redox activity and can participate in various biochemical reactions by accepting and donating electrons. As a trace element, thus, Anticancer effect of Copper Activated Plasma Water on MCF7 Breast Cancer Cells. Materials and method, used are a non-thermal micro-hollow cathode discharge (MHCD) was used to generate plasma-activated waters (CU-PAWs), The MCF-7 human breast adenocarcinoma cell line (IBRC C10082), and 3-(4,5 dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) was acquired. Cell Viability Measurements; After 48 h of incubation 0.5 mg/ml MTT (20 μl) was added to the control and experimental cells and the cells were incubated for 3 h in a CO2 incubator at 37 °C, Measurement of Reactive Oxygen Species (ROS) and Flow Cytometry was conducted. Results: The 3- and 4-min CU-PAW reduced MCF-7 cells viability to approximately 62% and 56% of control (p< 0.01), respectively. However, in the cases of 1- and 2-min CU-PAW cell proliferation did not diminish significantly as compared with the control group (p> 0.05). This observation is consistent with earlier studies, which illustrated that plasma irradiation reduced cell viability in a time-dependent manner. Thus, in this research, DOX (0.45 µM) combined with 3- or 4-min CU-PAW killed MCF-7 cell efficiently (44% and 39% cell viability, respectively; p< 0.01) than DOX (54% cell viability) or 3- or 4-min CU-PAW alone (63% and 56% cell viability, respectively). These was in line with a that PAW plus cisplatin at low doses reduced viability of human endometrial carcinoma more effectively than cisplatin or PAW alone. Conclusion: Although further investigations are crucial, CU-PAW combined with DOX could be a promising cancer treatment strategy, contributing to a more positive therapeutic agent.
Co-Authors Bilyaminu Habibu Daniel Konka Edom Favour Ogochukwu Isaac John Umaru Jackson Soyinka Malantso Joseph Oteng Kerenhappuch Isaac Umaru Okrah George Hassan Prince Kwadwo Gyasi Solomon Ossom Asare Tensaba Andes Akafa