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POTENSI Aneurinibacillus thermoaerophilus STRAIN N25 DALAM PRODUKSI BIOLISTRIK PADA VARIASI STARTER Echy Dania Putri; Irdawati; Azwir Anhar; Moralita Chatri
Jurnal Biogenerasi Vol. 11 No. 1 (2026): Volume 11, no 1, 2026, (Januari - Maret 2026)
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/ddks4p57

Abstract

The development of renewable energy sources is important due to the increasing demand for electricity. Microbial Fuel Cell (MFC) is a technology that utilizes microorganisms to convert organic substrates into electrical energy. This study aims to determine the potential of Aneurinibacillus thermoaerophilus strain N25 in producing bioelectricity at different starter concentrations. The research was conducted using an experimental method with variations of starter concentrations in an MFC system. Bioelectricity production was measured using a digital multimeter in millivolts (mV). The results are expected to provide information about the optimal starter concentration for bioelectricity production by thermophilic bacteria.
UJI AKTIVITAS ANTIMIKROBA BAKTERI ENDOFIT PSEUDOMONAS BERFLUORESEN TERHADAP Escherichia coli, Staphylococcus aureus DAN Candida Fauzi Eka Nuraini; Dwi Hilda Putri; Linda Advinda; Irdawati; Dezi Handayani; Laila Mardhiyah Nazri; Tesya Wulandari; Liza Febrianti
Jurnal Biogenerasi Vol. 11 No. 1 (2026): Volume 11, no 1, 2026, (Januari - Maret 2026)
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/biogenerasi.v11i1.7989

Abstract

Antimicrobial resistance is increasing every year. New and better antimicrobial sources are needed. One source of antimicrobials that can be explored is from endophytic bacteria. This study aims to test the ability of fluorescent Pseudomonas endophytic bacteria from the roots of plantain lemongrass in producing antimicrobial compounds. The type of research is descriptive. The antimicrobial activity of fluorescent Pseudomonas isolates was tested using the point inoculation method using test microbes E. coli, S. aureus and C. albicans. The clear zone was observed after 24 hours. The results showed that fluorescent Pseudomonas isolates were able to inhibit E. coli with a diameter of 3.9 mm found in isolate 51. S. aureus bacteria had an inhibition zone of 9.4 mm in isolate 89. Interesting results were shown by isolate 51 in inhibiting C. albicans with an inhibition zone diameter of 29.9 mm. These differences in response are thought to be related to variations in cell wall structure and the presence of an outer membrane in Gram-negative bacteria, which inhibits the penetration of active compounds. Overall, fluorescent Pseudomonas endophytic bacteria from the roots of the plantain have the potential to be developed as a source of new antifungal candidates against C. albicans, while their antibacterial activity still needs to be optimized through compound purification and further testing.
BIOLISTRIK RAMAH LINGKUNGAN DARI MIKROBA EKSOELEKTROGENS TANAH PUPUK ORGANIK PADA BERBAGAI PENAMBAHAN AIR Regina Zahrani Marza; Irdawati; Putri Amelya Ningsih; Muhammad Nazif Zen; Silvi Tamara; Echy Dania Putri; Dinda Aulia Nabila; Intan Feby Nasty
Jurnal Biogenerasi Vol. 11 No. 1 (2026): Volume 11, no 1, 2026, (Januari - Maret 2026)
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/biogenerasi.v11i1.8016

Abstract

The increasing demand for electricity has triggered the development of environmentally friendly alternative energy sources. One promising innovative technology is the Microbial Fuel Cell (MFC), which utilizes electroactive microorganisms such as exoelectrogens to generate electricity from organic materials. This research aims to analyze the impact of organic fertilizer soil and the addition of water on bi-electricity production in MFC systems. The experiment was conducted using organic fertilizer soil as a substrate, with parameters such as voltage and power density being measured regularly. The results show that exoelectrogenic microbes can significantly enhance electricity production, with specific treatments (P2 U1) providing the highest stability in electricity generation over 24 hours. The addition of water also positively impacted microbial activity and electron transfer. This study reinforces the potential of organic fertilizer soil-based MFCs as a sustainable and environmentally friendly renewable energy source.
ANALISIS KONTAMINASI JAMUR PADA KUNYIT GILING CURAH DI PASAR LUBUK BUAYA KOTA PADANG Zulia Putri Azzahra; Dezi Handayani; Moralita Chatri; Irdawati
Jurnal Biogenerasi Vol. 11 No. 1 (2026): Volume 11, no 1, 2026, (Januari - Maret 2026)
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/biogenerasi.v11i1.8185

Abstract

Keamanan pangan sangat penting untuk melindungi kesehatan masyarakat, khususnya untuk produk pangan yang rentan terhadap kontaminasi mikroba. Kunyit bubuk curah yang dijual di pasar tradisional berisiko terkontaminasi jamur karena praktik penanganan yang tidak higienis, yang dapat mengurangi kualitas produk dan menimbulkan bahaya kesehatan. Penelitian ini bertujuan untuk mengetahui tingkat kontaminasi jamur pada kunyit bubuk curah yang dijual di Pasar Lubuk Buaya, Kota Padang, dan untuk mengidentifikasi karakteristik makroskopis jamur yang tumbuh. Penelitian ini dilakukan dari Oktober 2025 hingga Januari 2026 di Laboratorium Mikrobiologi, Departemen Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Negeri Padang. Desain penelitian deskriptif digunakan dengan metode pengambilan sampel purposif. Sampel kunyit bubuk curah dipilih dan dikumpulkan dari lima pedagang dengan tingkat kebersihan yang berbeda. Kontaminasi jamur dinilai menggunakan metode penghitungan kapang dan ragi (MYC) dengan teknik spread plate pada media Potato Dextrose Agar (PDA), diikuti dengan pengamatan makroskopis morfologi koloni jamur. Hasil menunjukkan bahwa semua sampel kunyit bubuk curah terkontaminasi jamur, dengan tingkat kontaminasi berkisar antara 2,67 × 10⁵ hingga 2,9 × 10⁶ CFU/g. Semua sampel melebihi batas maksimum yang diizinkan untuk kontaminasi mikroba sebagaimana ditetapkan oleh Badan Pengawas Obat dan Makanan Indonesia (BPOM). Jamur kontaminan yang diidentifikasi meliputi Aspergillus flavus, A. niger, Penicillium sp., dan ragi. Oleh karena itu, dapat disimpulkan bahwa kunyit bubuk curah yang dijual di Pasar Lubuk Buaya, Kota Padang, tidak memenuhi standar keamanan pangan mikrobiologis.
POTENSI Bacillus licheniformis strain Nam 2-2 DALAM PRODUKSI BIOLISTRIK PADA VARIASI pH Silvi Tamara Silvi; Irdawati; Linda Advinda; Elsa Yuniarti
Jurnal Biogenerasi Vol. 11 No. 2 (2026): April - Juni 2026
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/55fxme08

Abstract

The increasing global energy demand has encouraged the development of sustainable alternative energy sources such as Microbial Fuel Cells (MFCs). This study aimed to determine the optimum pH for bioelectricity production using Bacillus licheniformis strain Nam 2-2 in a dual-chamber MFC system. The experiment employed a completely randomized design with pH variations of 7, 7.5, and 8. Voltage output was measured every 2 hours over a 24-hour incubation period. The results showed that bioelectricity production increased over time, reached a peak at 16–20 hours, and then declined. The highest voltage was observed at pH 7.5, reaching 0.782 mV, followed by pH 8 (0.758 mV) and pH 7 (0.748 mV). These findings indicate that slightly alkaline conditions are more favorable for microbial activity and electron transfer efficiency. Therefore, pH optimization is essential to improve MFC performance and supports its potential as a renewable energy source.
POLA PRODUKSI LISTRIK Bacillus licheniformis strain KGEB16 AMOBIL PADA SISTEM MICROBIAL FUEL CELL (MFC) fazdkia oktriani; Irdawati; Siska Alicia Farma; Fadilaturahmah
Jurnal Biogenerasi Vol. 11 No. 2 (2026): April - Juni 2026
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/8cvprk22

Abstract

Peningkatan kebutuhan energi berkelanjutan telah menunutut pengembangan Microbial Fuel Cell (MFC) sebagai teknologi alternatif. Penelitian ini bertujuan untuk menganalisis pola produksi listrik Bacillus licheniformis strain KGEB16 yang diamobilisasi pada sistem Microbial Fuel Cell (MFC). Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan perlakuan variasi konsentrasi natrium alginat (2%, 3%, 4%, 5%, dan 6%) serta lima ulangan. Proses amobilisasi sel dilakukan dengan metode entrapment menggunakan natrium alginat, sedangkan pengukuran tegangan listrik dilakukan setiap 2 jam selama periode inkubasi 24 jam. Data yang diperoleh dianalisis menggunakan uji ANOVA dan dilanjutkan dengan uji Duncan (Duncan Multiple Range Test/DMRT) pada taraf signifikansi 5%. Hasil penelitian menunjukkan bahwa pola produksi listrik mengalami peningkatan secara bertahap sejak awal inkubasi hingga mencapai puncak pada jam ke-20, kemudian menurun pada jam ke-22 hingga 24. Tegangan tertinggi diperoleh pada konsentrasi natrium alginat 5%, sedangkan tegangan terendah diperoleh pada konsentrasi 2%. Dengan demikian, dapat disimpulkan bahwa konsentrasi natrium alginat berpengaruh signifikan terhadap pola produksi listrik, dengan konsentrasi optimum sebesar 5% pada sistem MFC.
POLA PRODUKSI BIOLISTRIK Aneurinibacillus thermoaerophilus STRAIN N25  PADA VARIASI SUBSTRAT LIMBAH PERTANIAN Dinda Aulia Nabila; Irdawati; Dezi Handayani; Elsa Yuniarti
Jurnal Biogenerasi Vol. 11 No. 2 (2026): April - Juni 2026
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/2mcsjx62

Abstract

The increasing energy demand encourages the development of renewable energy technologies such as Microbial Fuel Cell (MFC), which converts organic substrates into electricity using microorganisms. This study aimed to analyze bioelectricity production by Aneurinibacillus thermoaerophilus strain N25 using different substrates (glucose, corn cob, rice straw, and rice husk). The experiment was conducted using a dual-chamber MFC system with voltage measurements over 24 hours. The results showed that voltage increased over time, peaked at the 20th hour, and then decreased. The highest voltage was produced by glucose (0.802 mV), followed by corn cob (0.724 mV), rice straw (0.581 mV), and rice husk (0.491 mV). These results indicate that substrate type affects bioelectricity production, with corn cob as the best agricultural waste substrate.
Analisis Produksi Biolistrik oleh Aneurinibacillus thermoaerophilus Strain N25 pada Variasi Konsentrasi Starter dalam Sistem Microbial Fuel Cell (MFC) Echy Dania Putri; Irdawati; Azwir Anhar; Moralita Chatri
Jurnal Biogenerasi Vol. 11 No. 2 (2026): April - Juni 2026
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/4102hx98

Abstract

The increasing demand for electrical energy drives the development of renewable energy technologies such as Microbial Fuel Cell (MFC), which converts organic substrates into electricity using microorganisms. This study aims to determine the effect of starter concentration and identify the optimal concentration of Aneurinibacillus thermoaerophilus strain N25 in bioelectricity production. An experimental method was used with a dual-chamber MFC system and starter concentrations of 3%, 6%, 9%, 12%, and 15%. Voltage production was measured using a digital multimeter (V) over 24 hours. The results showed that starter concentration affected bioelectricity production, with voltage increasing over time and reaching a peak at the 20th hour. Higher starter concentrations produced higher voltage due to increased microbial activity and more efficient electron transfer.
APLIKASI PENGGUNAAN BIOLISTRIK YANG DIHASILKAN OLEH MIKROBA Bacillus licheniformis strain NA14 PADA LAMPU LED Chetiarahmi Rahmarani; Irdawati
Jurnal Biogenerasi Vol. 11 No. 2 (2026): April - Juni 2026
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/t4pqtm35

Abstract

Kebutuhan energi listrik yang terus meningkat mendorong pengembangan sumber energi alternatif, salah satunya melalui teknologi Microbial Fuel Cell (MFC). Penelitian ini bertujuan untuk mengkaji kemampuan produksi energi listrik oleh bakteri termofilik Bacillus licheniformis strain NA14 dari sumber air panas Mudiak Sapan serta pengaplikasiannya dalam menyalakan lampu LED sebagai indikator biolistrik. Penelitian ini bersifat deskriptif dan dilakukan di Laboratorium Mikrobiologi FMIPA Universitas Negeri Padang pada Oktober 2025 hingga Februari 2026. Sistem MFC yang digunakan berupa bioreaktor dual - chamber dengan rangkaian seri tiga menggunakan elektroda zink sebagai anoda dan tembaga sebagai katoda, serta jembatan garam sebagai penghubung. Medium yang digunakan adalah Broth Thermophilic Medium (BTM) yang diinokulasikan dengan isolat bakteri termofilik. Hasil penelitian menunjukkan bahwa sistem MFC mampu menghasilkan tegangan sebesar 2,60 V sehingga dapat menyalakan lampu LED. Intensitas nyala LED dipengaruhi oleh aktivitas elektrogenik bakteri dalam mengoksidasi senyawa organik dan mentransfer elektron ke anoda. Selain itu, aktivitas enzimatik seperti xilanase serta pola pertumbuhan bakteri pada fase eksponensial dan stasioner turut mendukung peningkatan produksi listrik. Dengan demikian, Bacillus licheniformis strain NA14 berpotensi sebagai bioelectricigens dalam pengembangan teknologi MFC sebagai sumber energi alternatif.
Co-Authors Azwir Anhar Chetiarahmi Rahmarani Dezi Handayani Dinda Aulia Nabila Dinda Aulia Nabila Dwi Hilda Putri Echy Dania Putri Elsa Yuniarti Fadilaturahmah Fauzi Eka Nuraini fazdkia oktriani Intan Feby Nasty Laila Mardhiyah Nazri Linda Advinda Liza Febrianti Moralita Chatri Muhammad Nazif Zen Putri Amelya Ningsih Regina Zahrani Marza Silvi Tamara Silvi Tamara Silvi Siska Alicia Farma Tesya Wulandari Zulia Putri Azzahra