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Dwi Hilda Putri
State University of Padang
Education Languange, Linguistic, Communication & Media Other

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UJI AKTIVITAS ANTIMIKROBA BAKTERI ENDOFIT PSEUDOMONAS BERFLUORESEN TERHADAP Escherichia coli, Staphylococcus aureus DAN Candida Fauzi Eka Nuraini; Dwi Hilda Putri; Linda Advinda; Irdawati; Dezi Handayani; Laila Mardhiyah Nazri; Tesya Wulandari; Liza Febrianti
Jurnal Biogenerasi Vol. 11 No. 1 (2026): Volume 11, no 1, 2026, (Januari - Maret 2026)
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/biogenerasi.v11i1.7989

Abstract

Antimicrobial resistance is increasing every year. New and better antimicrobial sources are needed. One source of antimicrobials that can be explored is from endophytic bacteria. This study aims to test the ability of fluorescent Pseudomonas endophytic bacteria from the roots of plantain lemongrass in producing antimicrobial compounds. The type of research is descriptive. The antimicrobial activity of fluorescent Pseudomonas isolates was tested using the point inoculation method using test microbes E. coli, S. aureus and C. albicans. The clear zone was observed after 24 hours. The results showed that fluorescent Pseudomonas isolates were able to inhibit E. coli with a diameter of 3.9 mm found in isolate 51. S. aureus bacteria had an inhibition zone of 9.4 mm in isolate 89. Interesting results were shown by isolate 51 in inhibiting C. albicans with an inhibition zone diameter of 29.9 mm. These differences in response are thought to be related to variations in cell wall structure and the presence of an outer membrane in Gram-negative bacteria, which inhibits the penetration of active compounds. Overall, fluorescent Pseudomonas endophytic bacteria from the roots of the plantain have the potential to be developed as a source of new antifungal candidates against C. albicans, while their antibacterial activity still needs to be optimized through compound purification and further testing.
AKTIVITAS ANTIMIKROBA BAKTERI ENDOFIT (Pseudomonas Berfluoresen) DARI AKAR PISANG BUAI (Musa x paradisiaca L.) Astrid; Dwi Hilda Putri; Dezi Handayani
Jurnal Biogenerasi Vol. 11 No. 1 (2026): Volume 11, no 1, 2026, (Januari - Maret 2026)
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/biogenerasi.v11i1.8176

Abstract

Infectious diseases caused by pathogenic bacteria and fungi remain a global health problem, particularly due to the increasing incidence of antimicrobial resistance, highlighting the need for effective alternative antimicrobial sources. Endophytic bacteria, especially fluorescent Pseudomonas, are known to produce secondary metabolites with antibacterial and antifungal properties. This study aimed to evaluate the antimicrobial activity of endophytic fluorescent Pseudomonas isolated from the roots of banana buai (Musa × paradisiaca L.) and to identify isolates with the highest activity. This experimental study employed a Completely Randomized Design (CRD) using 44 isolates with three replications. Antimicrobial activity was tested in vitro using the spot inoculation method against Escherichia coli, Staphylococcus aureus, and Candida albicans. The observed parameter was the diameter of the inhibition zone, which was analyzed using analysis of variance (ANOVA) followed by Duncan’s Multiple Range Test. The results showed that antimicrobial activity varied among isolates and was specific to the test microorganisms. Isolate PFPB 32 exhibited the highest activity against E. coli and S. aureus, while isolate PFPB 300 showed the strongest antifungal activity against C. albicans. These findings indicate that endophytic fluorescent Pseudomonas from banana buai roots have potential as a source of antimicrobial agents, particularly antifungal agents against Candida albicans
Optimasi Primer : Suhu Annealing untuk Deteksi Gen Matrix Virus Influenza A Menggunakan  Polymerase Chain Reaction (PCR) Natalia Turnip; Nova Dilla Yanthi; Dwi Hilda Putri
Jurnal Biogenerasi Vol. 11 No. 2 (2026): April - Juni 2026
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/e9cf4f40

Abstract

Influenza A virus (IAV) is a pathogen that attacks the respiratory tract in humans and animals, which can be a threat to public health. This virus has a number of genes that are targeted by PCR for diagnostic detection purposes, one of which is the matrix gene. Detection of the matrix gene in the Influenza A virus from DNA/RNA genetic material can be done using the polymerase chain reaction (PCR) technique. One of the stages that determines the success of the PCR process is the annealing stage, for which the optimum temperature and cycle are required for primer attachment in the annealing stage. This research is a descriptive study towards an experiment conducted in June 2025 at the Bacteriology Laboratory of the Research and Innovation Agency (BRIN) KST. Soekarno Cibinong. The results obtained showed that the optimum temperature for matrix gene amplification in the Influenza A virus using the forward primer FluAV M-U44 and reverse primer FluAV M-L287 was 53.5 °C, 54 °C, and 54,5 °C and 35 cycles were evidenced by the formation of a fairly bright band in the electrophoresis results.
LITERATURE REVIEW: KARAKTERISTIK DAN DISTRIBUSI CORONAVIRUS PADA KELELAWAR SEBAGAI RESERVOIR ALAMI Desrinda Dwi Adinda Putri; Sugiyono Saputra; Dwi Hilda Putri
Jurnal Biogenerasi Vol. 11 No. 2 (2026): April - Juni 2026
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/azkxbc74

Abstract

Coronavirus merupakan virus RNA untai tunggal positif yang memiliki keragaman genetik tinggi dan mampu berevolusi melalui mutasi serta rekombinasi. Kelelawar diketahui sebagai reservoir alami coronavirus karena memiliki sistem imun yang unik serta karakteristik ekologi yang mendukung penyebaran virus. Penelitian ini bertujuan untuk menganalisis dan mensintesis berbagai hasil penelitian mengenai karakteristik dan distribusi coronavirus pada kelelawar. Metode yang digunakan adalah literature review dengan mengumpulkan artikel dari Google Scholar, PubMed, dan ScienceDirect dalam sepuluh tahun terakhir. Hasil kajian menunjukkan bahwa coronavirus pada kelelawar memiliki keragaman genetik yang tinggi, terutama pada kelompok Alphacoronavirus dan Betacoronavirus, serta tersebar luas di berbagai wilayah, khususnya Asia Tenggara. Beberapa penelitian juga menunjukkan adanya potensi transmisi lintas spesies (spillover) yang dipengaruhi oleh adaptasi virus dan faktor lingkungan. Kesimpulannya, kelelawar berperan penting dalam menjaga keragaman dan distribusi coronavirus, sehingga diperlukan pemantauan yang berkelanjutan untuk mencegah munculnya penyakit zoonotik di masa depan.
LITERATUR REVIEW: APLIKASI MOLEKULER BERBASIS PCR DALAM DETEKSI  DAN KARAKTERISASI Escherichia coli PADA SEKTOR LINGKUNGAN, PANGAN, DAN KLINIS Diva Yunavita; Dwi Hilda Putri
Jurnal Biogenerasi Vol. 11 No. 2 (2026): April - Juni 2026
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/jcp1q263

Abstract

Escherichia coli is a gram-negative bacterium and a key indicator of water and food contamination. Conventional PCR is a widely used molecular method for specific, rapid, and sensitive E. coli detection. This literature review systematically examines the application of conventional PCR in E. coli detection across water, food, and clinical sample matrices from 2017 to 2025. Thirteen scientific articles were reviewed regarding research objects, PCR methods, research stages, and findings. Conventional PCR using primers 16E1/16E2 (584 bp), Afa FP/RP (480 bp), and ESS (825 bp) effectively detected E. coli in diverse samples. PCR showed superior sensitivity (100%) and specificity (94.34%) compared to culture methods (Hariri, 2022). Direct PCR offered a cost-effective alternative without DNA extraction (JKG, 2025). Multiplex PCR enabled simultaneous detection of multiple virulence genes (Ekawati et al.; Microbiology Spectrum, 2024). Conventional PCR remains a reliable, specific, and rapid tool for E. coli detection across diverse sample types.
Co-Authors Astrid Desrinda Dwi Adinda Putri Dezi Handayani Diva Yunavita Fauzi Eka Nuraini Irdawati Laila Mardhiyah Nazri Linda Advinda Liza Febrianti Natalia Turnip Nova Dilla Yanthi Sugiyono Saputra Tesya Wulandari