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Dezi Handayani
State University of Padang
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LITERATUR REVIEW: APLIKASI EKSTRAK TANAMAN DALAM MENGENDALIKAN PENYAKIT BUSUK PANGKAL BATANG YANG DISEBABKAN JAMUR PATOGEN GANODERMA BONINENSE Muthia Ridala; Dezi Handayani
Jurnal Biogenerasi Vol. 11 No. 3 (2026): Juli - September 2026
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/fdg31430

Abstract

Stem base rot in oil palm caused by Ganoderma boninense is one of the main factors contributing to reduced productivity. Currently, control still relies on synthetic fungicides. However, their continuous use has the potential to cause negative environmental impacts. Therefore, more environmentally friendly control alternatives are needed, one of which is through the use of plant extracts. This study aims to assess the potential of plant extracts as antifungal agents in controlling basal stem rot base on a systematic literature review. The method used is a Systematic Literature Review (SLR) following the PRISMA guidelines through the steps of identification, screening, eligibility, and included. Data sources were obtained from various platforms such as Google Scholar, SpringerLink, and ScienceDirect, with article inclusion criteria ranging from 2016 2026. The results of the review indicate that various plant extracts, such as Asystasia gangetica, Curcuma longa, Cerbera manghas, Senna multijuga, and Elettaria cardamomum, exhibit antifungal activity against G. boninense. Their effectiveness is influenced by the plant species, the part used, the extraction method, the solvent, and the concetration. The mechanisms of action include cell membrane damage, disruption of permeability, and inhibition of pathogenic enzymes. Thus, plant extracs have the potential to be developed as an effective, environmentally friendly and sustainable botanical fungicide alternative for controlling basal stem rot in oil palm.
UJI AKTIVITAS ANTIMIKROBA BAKTERI ENDOFIT PSEUDOMONAS BERFLUORESEN TERHADAP Escherichia coli, Staphylococcus aureus DAN Candida Fauzi Eka Nuraini; Dwi Hilda Putri; Linda Advinda; Irdawati; Dezi Handayani; Laila Mardhiyah Nazri; Tesya Wulandari; Liza Febrianti
Jurnal Biogenerasi Vol. 11 No. 1 (2026): Volume 11, no 1, 2026, (Januari - Maret 2026)
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/biogenerasi.v11i1.7989

Abstract

Antimicrobial resistance is increasing every year. New and better antimicrobial sources are needed. One source of antimicrobials that can be explored is from endophytic bacteria. This study aims to test the ability of fluorescent Pseudomonas endophytic bacteria from the roots of plantain lemongrass in producing antimicrobial compounds. The type of research is descriptive. The antimicrobial activity of fluorescent Pseudomonas isolates was tested using the point inoculation method using test microbes E. coli, S. aureus and C. albicans. The clear zone was observed after 24 hours. The results showed that fluorescent Pseudomonas isolates were able to inhibit E. coli with a diameter of 3.9 mm found in isolate 51. S. aureus bacteria had an inhibition zone of 9.4 mm in isolate 89. Interesting results were shown by isolate 51 in inhibiting C. albicans with an inhibition zone diameter of 29.9 mm. These differences in response are thought to be related to variations in cell wall structure and the presence of an outer membrane in Gram-negative bacteria, which inhibits the penetration of active compounds. Overall, fluorescent Pseudomonas endophytic bacteria from the roots of the plantain have the potential to be developed as a source of new antifungal candidates against C. albicans, while their antibacterial activity still needs to be optimized through compound purification and further testing.
KEMAMPUAN PSEUDOMONAS BERFLUORESEN SEBAGAI AGEN ANTAGONIS TERHADAP Ralstonia solanaceaerum PENYEBAB LAYU TANAMAN NILAM (Pogestemon cablin Benth) Abil Pratama Putra; Linda Advinda; Moralita Chatri; Dezi Handayani
Jurnal Biogenerasi Vol. 11 No. 1 (2026): Volume 11, no 1, 2026, (Januari - Maret 2026)
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/biogenerasi.v11i1.8088

Abstract

Patchouli (Pogestemon cablin Benth) is a cultivated plant that can produce oil and generate income for farmers. However, attacks by plant pests (OPT) often pose a challenge to patchouli production in Indonesia. One organism that causes wilt disease in patchouli plants is the bacterium Ralstonia solanaceaerum. Therefore, more environmentally friendly and sustainable control methods are needed. One strategy is the use of biological agents, especially antagonistic rhizosphere bacteria such as fluorescent pseudomonas. This study was conducted from June to August 2025 at the Plant Physiology Laboratory, Department of Biology, Faculty of Mathematics and Natural Sciences, Padang State University. This study used a completely randomized design (CRD) as the experimental design. In this study, there were 6 treatments (different isolates), each repeated 3 times. The parameter observed was the formation of an inhibition zone using the disc diffusion method. The data were analyzed using analysis of variance (ANOVA). If there were significant differences, Duncan's New Multiple Range Test (DNMRT) was used at a 5% level. Based on the results and discussions, it can be concluded that all fluorescent Pseudomonas isolates tested have the ability to inhibit R. solanaceaerum, the cause of wilt disease in patchouli plants. The isolate with the best ability to inhibit the growth of R. solanaceaerum on patchouli plants was isolate Pf S36 with an inhibition zone of 20.37 mm, but there was no significant difference between the other isolates because the inhibition effectiveness was relatively uniform.
AKTIVITAS ANTIMIKROBA BAKTERI ENDOFIT (Pseudomonas Berfluoresen) DARI AKAR PISANG BUAI (Musa x paradisiaca L.) Astrid; Dwi Hilda Putri; Dezi Handayani
Jurnal Biogenerasi Vol. 11 No. 1 (2026): Volume 11, no 1, 2026, (Januari - Maret 2026)
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/biogenerasi.v11i1.8176

Abstract

Infectious diseases caused by pathogenic bacteria and fungi remain a global health problem, particularly due to the increasing incidence of antimicrobial resistance, highlighting the need for effective alternative antimicrobial sources. Endophytic bacteria, especially fluorescent Pseudomonas, are known to produce secondary metabolites with antibacterial and antifungal properties. This study aimed to evaluate the antimicrobial activity of endophytic fluorescent Pseudomonas isolated from the roots of banana buai (Musa × paradisiaca L.) and to identify isolates with the highest activity. This experimental study employed a Completely Randomized Design (CRD) using 44 isolates with three replications. Antimicrobial activity was tested in vitro using the spot inoculation method against Escherichia coli, Staphylococcus aureus, and Candida albicans. The observed parameter was the diameter of the inhibition zone, which was analyzed using analysis of variance (ANOVA) followed by Duncan’s Multiple Range Test. The results showed that antimicrobial activity varied among isolates and was specific to the test microorganisms. Isolate PFPB 32 exhibited the highest activity against E. coli and S. aureus, while isolate PFPB 300 showed the strongest antifungal activity against C. albicans. These findings indicate that endophytic fluorescent Pseudomonas from banana buai roots have potential as a source of antimicrobial agents, particularly antifungal agents against Candida albicans
ANALISIS KONTAMINASI JAMUR PADA KUNYIT GILING CURAH DI PASAR LUBUK BUAYA KOTA PADANG Zulia Putri Azzahra; Dezi Handayani; Moralita Chatri; Irdawati
Jurnal Biogenerasi Vol. 11 No. 1 (2026): Volume 11, no 1, 2026, (Januari - Maret 2026)
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/biogenerasi.v11i1.8185

Abstract

Keamanan pangan sangat penting untuk melindungi kesehatan masyarakat, khususnya untuk produk pangan yang rentan terhadap kontaminasi mikroba. Kunyit bubuk curah yang dijual di pasar tradisional berisiko terkontaminasi jamur karena praktik penanganan yang tidak higienis, yang dapat mengurangi kualitas produk dan menimbulkan bahaya kesehatan. Penelitian ini bertujuan untuk mengetahui tingkat kontaminasi jamur pada kunyit bubuk curah yang dijual di Pasar Lubuk Buaya, Kota Padang, dan untuk mengidentifikasi karakteristik makroskopis jamur yang tumbuh. Penelitian ini dilakukan dari Oktober 2025 hingga Januari 2026 di Laboratorium Mikrobiologi, Departemen Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Negeri Padang. Desain penelitian deskriptif digunakan dengan metode pengambilan sampel purposif. Sampel kunyit bubuk curah dipilih dan dikumpulkan dari lima pedagang dengan tingkat kebersihan yang berbeda. Kontaminasi jamur dinilai menggunakan metode penghitungan kapang dan ragi (MYC) dengan teknik spread plate pada media Potato Dextrose Agar (PDA), diikuti dengan pengamatan makroskopis morfologi koloni jamur. Hasil menunjukkan bahwa semua sampel kunyit bubuk curah terkontaminasi jamur, dengan tingkat kontaminasi berkisar antara 2,67 × 10⁵ hingga 2,9 × 10⁶ CFU/g. Semua sampel melebihi batas maksimum yang diizinkan untuk kontaminasi mikroba sebagaimana ditetapkan oleh Badan Pengawas Obat dan Makanan Indonesia (BPOM). Jamur kontaminan yang diidentifikasi meliputi Aspergillus flavus, A. niger, Penicillium sp., dan ragi. Oleh karena itu, dapat disimpulkan bahwa kunyit bubuk curah yang dijual di Pasar Lubuk Buaya, Kota Padang, tidak memenuhi standar keamanan pangan mikrobiologis.
KERAGAMAN JAMUR MAKROSKOPIS DI SEKITAR TITIK PEMASANGAN KAMERA TRAP DI KAWASAN HUTAN NAGARI PARIAN LUNANG, PESISIR SELATAN Fichi Azra Syaqila; Dezi Handayani
Jurnal Biogenerasi Vol. 11 No. 2 (2026): April - Juni 2026
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/jenzcq83

Abstract

Indonesia is a tropical country with a high level of biodiversity, including the diversity of macrofungi which play an important role as decomposers in the process of organic matter decomposition and in maintaining ecosystem balance. The Nagari Parian Lunang Forest Area, Pesisir Selatan, West Sumatra, has humid and relatively natural environmental conditions that potentially support the growth of various species of macrofungi. This study aims to identify the species of macrofungi, determine their potential uses, and analyze the influence of environmental factors on their distribution patterns. This research was a descriptive study conducted from September to December 2025 using an exploration method at several camera trap installation points. The mushrooms found were recorded based on their morphological characteristics, documented, and identified using the Collins Fungi Guide and related scientific literature. Environmental parameters measured included light intensity, soil temperature, and air humidity. The results showed that 28 species of macrofungi were successfully identified. Several species have potential uses as food sources, traditional medicine, and producers of bioactive compounds such as antibiotics and antitumor agents. The results of the Canonical Correspondence Analysis (CCA) indicated that light intensity, soil temperature, and air humidity influence the distribution patterns of mushrooms at each observation point.
POLA PRODUKSI BIOLISTRIK Aneurinibacillus thermoaerophilus STRAIN N25  PADA VARIASI SUBSTRAT LIMBAH PERTANIAN Dinda Aulia Nabila; Irdawati; Dezi Handayani; Elsa Yuniarti
Jurnal Biogenerasi Vol. 11 No. 2 (2026): April - Juni 2026
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/2mcsjx62

Abstract

The increasing energy demand encourages the development of renewable energy technologies such as Microbial Fuel Cell (MFC), which converts organic substrates into electricity using microorganisms. This study aimed to analyze bioelectricity production by Aneurinibacillus thermoaerophilus strain N25 using different substrates (glucose, corn cob, rice straw, and rice husk). The experiment was conducted using a dual-chamber MFC system with voltage measurements over 24 hours. The results showed that voltage increased over time, peaked at the 20th hour, and then decreased. The highest voltage was produced by glucose (0.802 mV), followed by corn cob (0.724 mV), rice straw (0.581 mV), and rice husk (0.491 mV). These results indicate that substrate type affects bioelectricity production, with corn cob as the best agricultural waste substrate.
Co-Authors Abil Pratama Putra Astrid Dinda Aulia Nabila Dwi Hilda Putri Elsa Yuniarti Fauzi Eka Nuraini Fichi Azra Syaqila Irdawati Laila Mardhiyah Nazri Linda Advinda Liza Febrianti Moralita Chatri Muthia Ridala Tesya Wulandari Zulia Putri Azzahra