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All Journal JRST (Jurnal Riset Sains dan Teknologi) Bioscientist : Jurnal Ilmiah Biologi
Suci Indah Budiarti
Politeknik Yakpermas Banyumas
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Education Engineering Immunology & microbiology Social Sciences

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Soil-Derived Endospore-Forming Bacillus Bacteria Producing Protease Mazidah Noer Inayah; Suci Indah Budiarti; Rizal Khoirun Alfisah
JRST (Jurnal Riset Sains dan Teknologi) Volume 9 No. 2 September 2025: JRST
Publisher : Universitas Muhammadiyah Purwokerto

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30595/jrst.v9i2.26600

Abstract

Proteases are widely utilized in many industries, including waste treatment, textiles, detergents, food processing, and medicines. This encourages researchers to discover novel sources of these enzymes. Exploration and isolation of putative proteolytic bacteria from the soil is one promising approach. The purpose of this study is expected providing the bacterial isolates that will produce superior proteases. Proteolytic bacteria were isolated from soil samples using the spread plate technique and selective skim milk agar media. The formation of clear zones and the determination of the proteolytic index were the starting point for a qualitative analysis of protease activity. Protease activity was determined quantitatively. The substrate is 1% casein dissolved in a 0.2 M tris-HCl buffer at pH 7.5, while the standard is tyrosine. The quantitative measurement of protease activity was carried out concurrently with the bacterial growth curve determination. The proteolytic bacteria P02 was successfully isolated in this research, having a protease enzyme activity of 0.867 U/mL and a proteolytic index of 0.8 ± 0.1. Protease activity peaked when the bacterial growth was in the logarithmic phase. Proteolytic bacterial P02 was Gram-positive and possesses the ability to form endospores and has a rod-shaped cell morphology. According to the outcomes of biochemical physiological testing, the bacterial P02 is considered to be the genus of Bacillus sp. The proteolytic bacteria P02 has potential to provide a sustainable and renewable source of protease, which is widely employed in industry and biotechnology.
Isolasi dan Identifikasi Molekuler Bakteri Salmonella Asal Tempe Dage Berdasarkan Gen 16S rRNA Mazidah Noer Inayah; Suci Indah Budiarti; Rahayu Wulan
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 4 (2025): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i4.18200

Abstract

This study aimed to isolate Salmonella from tempe dage, particularly products sold in traditional markets in the Banyumas region, Central Java, and to identify the isolates through molecular analysis of the 16S rRNA gene. Isolation of Salmonella from dage samples was conducted using culture-based methods on several types of selective media specific for Salmonella. Identification was performed through biochemical characterization using the API 20E system and molecular analysis based on PCR amplification of the 16S rRNA gene. The resulting 16S rRNA sequences were subsequently analyzed using the BLAST tool available on the NCBI platform, and phylogenetic relationships were reconstructed with MEGA X. The study successfully obtained one bacterial isolate (TD.1), exhibiting transparent colonies with black centers on SSA medium. Biochemical tests including catalase activity, sugar fermentation, H₂S production, and citrate utilization showed positive results. Further identification using the API 20E system confirmed that isolate TD.1 belonged to the genus Salmonella. Molecular analysis of the 16S rRNA gene identified isolate TD.1 as Salmonella enterica. In conclusion, isolate TD.1, recovered from tempe dage was identified as Salmonella enterica. The presence of Salmonella enterica in dage highlights a potential health risk and underscores the need for improved food hygiene in traditional markets.
Co-Authors Alfisah, Rizal Khoirun Inayah, Mazidah Noer Rahayu Wulan