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All Journal Biology, Medicine, & Natural Product Chemistry Jurnal Redoks : Jurnal Pendidikan Kimia dan Ilmu Kimia
Holisha Widiyanto
Sekolah Tinggi Analis Kimia Cilegon
Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education Environmental Science Materials Science & Nanotechnology Medicine & Pharmacology Public Health

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The Effect of Methanol Concentrations Against Phenolic Total Content and Antioxidant of Balakacida Leaves (Chromolaena odorata) Sriwijayanti Sriwijayanti; Ahmad Firdaus; Boima Situmeang; Holisha Widiyanto; Dian Susvira
Biology, Medicine, & Natural Product Chemistry Vol 15, No 1 (2026)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2026.151.239-244

Abstract

The balakacida plant with the Latin name Chromolaena odorata is a plant that lives in a tropical climate. The balakacida plant is a plant of the Asteraceae Family. Phytochemical tests on balakacida leaves showed the presence of secondary metabolites of the tannin, phenol, flavonoid, saponin and steroid groups. This study aims to extract balakacida leaves and test the total phenolic and antioxidant content. Sample extraction was carried out by the maceration method using methanol solvent. Testing of total phenolic content using the Folin Ciocalteu method, while testing of antioxidant content using the DPPH method using a UV-Vis spectrophotometer. The results showed that the concentration of methanol solvent significantly affected the acquisition of total phenolics and antioxidants. The highest total phenolic content was obtained at a methanol extract concentration of 80% of 145.64 mg GAE/g. For antioxidant content, a methanol extract concentration of 60% showed the highest activity with an average DPPH % inhibition of 59.77%. It can be concluded that a methanol extract concentration of 60% is the optimal concentration to produce balakacida leaf extract with the best antioxidant activity, although not with the highest total phenolic content.
PERBANDINGAN AKTIVITAS ANTIOKSIDAN EKSTRAK METANOL KULIT BATANG GANDARIA DENGAN METODE DPPH DAN ABTS Boima Situmeang; Nurhayati Bialangi; Weny JA Musa; Ahmad Kadir Kilo; Holisha Widiyanto; Dian Susvira; Nani Yulianti
JURNAL REDOKS : JURNAL PENDIDIKAN KIMIA DAN ILMU KIMIA Vol 8 No 2 (2025): Jurnal Redoks : Jurnal Pendidikan Kimia dan Ilmu Kimia
Publisher : Program Studi Pendidikan Kimia STKIP Bima

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33627/re.v8i2.3323

Abstract

Penelitian ini bertujuan untuk membandingkan aktivitas antioksidan ekstrak metanol kulit batang gandaria (Bouea macrophylla Griff.) menggunakan dua metode pengujian, yaitu DPPH (2,2-diphenyl-1-picrylhydrazyl) dan ABTS (2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)). Ekstraksi dilakukan secara maserasi menggunakan pelarut metanol. Aktivitas antioksidan ditentukan berdasarkan nilai IC50, yaitu konsentrasi yang diperlukan untuk meredam 50% radikal bebas. Hasil uji menunjukkan bahwa ekstrak memiliki aktivitas antioksidan sangat kuat dengan nilai IC50 sebesar 12,259 ± 0,232 ppm untuk metode DPPH dan 15,831 ± 1,550 ppm untuk metode ABTS. Nilai IC50 yang lebih rendah pada metode DPPH menunjukkan bahwa ekstrak memiliki kemampuan yang lebih tinggi dalam menangkap radikal bebas pada metode tersebut. Hasil ini menunjukkan bahwa kulit batang gandaria berpotensi sebagai sumber antioksidan alami yang efektif dengan kemampuan merangkal radikal bebas DPPH dan ABTS sangat kuat.
Co-Authors Ahmad Firdaus Ahmad Kadir Kilo Boima Ramses Situmeang Boima Situmeang Dian Susvira Nani Yulianti Nurhayati Bialangi Sriwijayanti Sriwijayanti