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All Journal Pharmacy Reports Scientific Nexus
Refsya Azanti Putri
Department of Pharmacy, Faculty of Science, Institut Teknologi Sumatera, South Lampung 35365, Indonesia
Astronomy Biochemistry, Genetics & Molecular Biology Chemistry Earth & Planetary Sciences Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology

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Analgetic effectiveness of fenugreek seed extract (Trigonella foenum-graecum L.) in acetic acid-induced male ddY strain mice Riska Okta Via; Dewi Damayanti Abdul Karim; Andreanus Andaja Soemardji; Refsya Azanti Putri
Scientific Nexus Vol. 1 No. 1 (2025): Scientific Nexus
Publisher : Fakultas Sains Institut Teknologi Sumatera

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35472/scinexus.2254

Abstract

Pain represents a significant health challenge, with current nonsteroidal anti-inflammatory drugs (NSAIDs) carrying hepatotoxicity risks that necessitate safer alternatives. This study evaluated the analgesic activity of fenugreek (Trigonella foenum-graecum L.) seed extract using the acetic acid-induced writhing test in male ddY strain mice. Twenty-five mice were randomly allocated into five groups (n=5): negative control (1% Na-CMC), positive control (diclofenac sodium 50 mg/kg), and three fenugreek extract groups (200, 400, and 600 mg/kg). Pain was induced by intraperitoneal injection of 1% acetic acid, and writhing episodes were counted for 180 minutes. Data were analyzed using one-way ANOVA with Tukey's post-hoc test. All fenugreek doses significantly reduced writhing compared to negative control (p < 0.05). The extract demonstrated dose-dependent analgesic activity, with protection rates of 30.8%, 35.9%, and 41.0% for 200, 400, and 600 mg/kg doses, respectively. The highest dose (600 mg/kg) achieved 71% of diclofenac's analgesic efficacy (46.2% protection). Phytochemical screening confirmed the presence of alkaloids, flavonoids, saponins, steroids, tannins, and polyphenols. These findings suggest fenugreek seed extract possesses significant analgesic properties and represents a promising natural alternative for pain management.
In silico screening of cucurbitacin variants identifies 11-deoxycucurbitacin I as a candidate ligand for the NACHT domain of NLRP3 Sarmoko; Ahmad Zammi Autadan Hakim; Nisa Yulianti Suprahman; Refsya Azanti Putri; Muhammad Yogi Saputra; Tantri Liris Nareswari; Manami Toriyama
Pharmacy Reports Vol. 6 No. 2 (2026): Pharmacy Reports
Publisher : Indonesian Young Scientist Group and UPN Veteran Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51511/pr.125

Abstract

Chronic inflammation contributes to a wide range of diseases, driving the need for novel anti-inflammatory agents with minimal side effects. The NACHT domain of NLRP3 mediates ATP-dependent inflammasome assembly and represents a validated target for anti-inflammatory therapy. This study aimed to predict and compare the binding interactions of twenty-two cucurbitacin variants against the NACHT domain using molecular docking, following geometry optimization with Density Functional Theory (B3LYP/6-31G(d)). Docking validation reproduced the native ligand pose with an RMSD of 0.87 angstrom. Among all variants tested, 11-deoxycucurbitacin I showed the most favorable predicted binding energy (-7.38 kcal/mol), sharing several interacting residues with the native ligand RM5, including Ala227, Ala228, Pro352, Ile411, Phe575, and Met661, although its predicted affinity remained weaker than that of RM5 (-10.35 kcal/mol). These shared contacts suggest that 11-deoxycucurbitacin I may engage a similar region of the NACHT inhibitor-binding pocket as RM5. In conclusion, 11-deoxycucurbitacin I is identified as the most favorable predicted binder among the cucurbitacin variants tested toward the NACHT domain, representing a candidate warranting further experimental validation.
Co-Authors Ahmad Zammi Autadan Hakim Andreanus Andaja Soemardji Dewi Damayanti Abdul Karim Manami Toriyama Muhammad Yogi Saputra Nisa Yulianti Suprahman Riska Okta Via Sarmoko Tantri Liris Nareswari