Background: Dengue fever is an infectious disease caused by the dengue virus and transmitted through the bites of Aedes aegypti and Aedes albopictus mosquitoes. The disease continues to pose a major public health challenge, particularly in tropical and subtropical regions, including Indonesia. This study aims to examine the Health Belief Model in relation to dengue prevention behaviors and the incidence of dengue fever in Jepara District, Central Java. Subjects and Method: This was a cross-sectional study conducted in Jepara District, Central Java, in January 2025. A sample of 200 respondents was selected using the fixed disease sampling method, all of whom were residents of Jepara District. The dependent variable was dengue prevention behavior. Independent variables included perceived susceptibility, perceived severity, perceived benefits, perceived barriers, cues to action, and self-efficacy. The data were collected using a questionnaire and analyzed using multiple linear analysis. Results: Dengue prevention behavior increased with higher perceived susceptibility (b = 0.21; 95% CI = 0.00 to 0.43; p = 0.049), perceived severity (b = 0.29; 95% CI = 0.01 to 0.57; p = 0.044), perceived benefits (b= 0.33; 95% CI= 0.03 to 0.63; p = 0.030), cues to action (b = 0.19; 95% CI = 0.01 to 0.37; p = 0.042), and self-efficacy (b= 0.33; 95% CI = 0.01 to 0.65; p = 0.045), while lower perceived barriers were associated with improved prevention behavior (b = –0.18; 95% CI = –0.36 to –0.01; p = 0.045). Conclusion: The results of this study show that higher levels of perceived susceptibility, perceived severity, perceived benefits, cues to action, and self-efficacy, as well as lower perceived barriers, enhance dengue prevention behaviors.