Chemistry Indonesian Journal of Chemical Research
Vol 3 No 2 (2016): Edisi Bulan Januari (Edition For January)

Produksi Oligomer Kitosan dari Limbah Udang Windu (Panaeus monodon) Menggunakan Enzim Kitosanase dari Isolat Bakteri Klebsiella sp.

Sarni, Sarni (Unknown)
Natsir, Hasnah (Unknown)
Dali, Seniwati (Unknown)



Article Info

Publish Date
31 Jan 2016

Abstract

Chitosan is a biopolymer that is the main content of D-glucosamine and several parts of N-acetyl-D-glucosamine bound to β- (1-4) glucoside. Chitosan receive special attention as functional biopolymers for applications in various fields. Chitosan is more effectively absorbed into the human body when it gets converted into chitosan oligomer form. Chitosan oligomer is a mixture of oligomers of D-glucosamine are formed through a process of severing ties depolymerization of chitosan with β-glycosidic. This study aims to produce chitosan oligomer of waste tiger shrimp (Penaeus monodon) using enzyme kitosanase of bacteria Klebsiella sp. Chitosan oligomer produced by using the enzyme chitosanase at a temperature of 40 °C and pH 8 with the activity of 0.309 U/mL (5,235 U/mg) obtained in the form of a mixture of monomer to octamer, which soluble in acetic acid 0.25% to 0.5%, having intrinsic viscosity decreases with increasing time of incubation is 0.195 (1 hour incubation); 0.9 (incubation 2 hours) and 0.7 (incubation 3 hours) with molecular weight range of 4103.12 g/mol (incubation 1 hour) ; 1483.48 g/mol (incubation 2 hours) and 1065.79 g/mol (incubation 3 hours).

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Journal Info

Abbrev

Indonesian Journal of Chemical Research (Indones.

Publisher

Subject

Biochemistry, Genetics & Molecular Biology Chemistry Engineering Environmental Science

Description

Indo. J. Chem. Res. is a journal that regularly publishes primary articles covering all branches of chemistry and its sub-disciplines. Published articles consist of complete research, short communication, and review articles. The scope of published articles is not limited to inorganic chemistry, ...