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All Journal The Journal of Pure and Applied Chemistry Research As-syifaa Jurnal Farmasi Window of Health : Jurnal Kesehatan Chemistry Indonesian Journal of Chemical Research Molekul: Jurnal Ilmiah Kimia
Seniwati Dali
Jurusan Kimia, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Hasanuddin
Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Engineering Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Nursing Public Health

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Total Flavonoid Levels of Ethanol Extract of Lemongrass Leaves and Thatch Grass Leaves Using UV-VIS Spectrophotometry Nuryadin, Yudhi; Naid, Tadjuddin; Dahlia, Andi Amaliah; Dali, Seniwati
Window of Health : Jurnal Kesehatan Vol. 1 No. 4 (Oktober, 2018)
Publisher : Fakultas Kesehatan Masyarakat Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (702.445 KB)

Abstract

Lemongrass Leaves (Cymbopogoncitratus DC.) and Thatch Grass Leaves (Imperata cylindrical (L.)Raeusch) are medicinal plants belong to similary family. Their activity as natural antioxidant sources because it’s contains the flavonoids compound. This study aimed to determine the total flavonoid content on the ethanol extract of lemongrass leaves and the thatch grass leaves using UV-Vis spectrophotometry. The extraction wasdone by maceration methodusing ethanol. The first sample is 3,95% lemongrass leaves, and the second one is 2,98% thatch grass leaves. The qualitative analysis by TLC method with the eluent ratio n-Hexane:ethyl acetate (6:4) showed that both samples positively contain flavonoids. The quantitative analysis by spectrophotometry at the maximum wavelength 431 nm showed that total flavonoid content in the first sample is 2,8666 % and the second sample is 3,4866 %.
Total Flavonoid Levels of Ethanol Extract of Lemongrass Leaves and Thatch Grass Leaves Using UV-VIS Spectrophotometry Nuryadin, Yudhi; Naid, Tadjuddin; Dahlia, Andi Amaliah; Dali, Seniwati
Window of Health : Jurnal Kesehatan Vol. 1 No. 4 (Oktober, 2018)
Publisher : Fakultas Kesehatan Masyarakat Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (702.445 KB) | DOI: 10.33368/woh.v1i4.107

Abstract

Lemongrass Leaves (Cymbopogoncitratus DC.) and Thatch Grass Leaves (Imperata cylindrical (L.)Raeusch) are medicinal plants belong to similary family. Their activity as natural antioxidant sources because it’s contains the flavonoids compound. This study aimed to determine the total flavonoid content on the ethanol extract of lemongrass leaves and the thatch grass leaves using UV-Vis spectrophotometry. The extraction wasdone by maceration methodusing ethanol. The first sample is 3,95% lemongrass leaves, and the second one is 2,98% thatch grass leaves. The qualitative analysis by TLC method with the eluent ratio n-Hexane:ethyl acetate (6:4) showed that both samples positively contain flavonoids. The quantitative analysis by spectrophotometry at the maximum wavelength 431 nm showed that total flavonoid content in the first sample is 2,8666 % and the second sample is 3,4866 %.
Aktivitas Antioksidan Ekstrak Etanol Buah Merah (Pandanus conoideus Lam.) dengan Metode FRAP Wabula, Rheytno Asdin; Dali, Seniwati; Widiastuti, Harti
Window of Health : Jurnal Kesehatan Vol. 2 No. 4 (Oktober, 2019)
Publisher : Fakultas Kesehatan Masyarakat Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (901.39 KB) | DOI: 10.33368/woh.v0i0.203

Abstract

Red fruit (Pandanus conoideus Lam.) Is an endemic plant that grows in the Papua region. Based on previous research, red fruit contains several active compounds with a fairly high level, including carotenoids, tocopherol, beta-carotene, α tocopherol, and fatty acids such as oleic acid, linoleic acid, linolenic acid, and dexoic acid. The high tocopherol content in red fruit has antioxidant activity. This study aims to determine the antioxidant capacity of the ethanol extract of red fruit (Pandanus conoideus Lam.) Using the FRAP method. Three replications of red fruit ethanol extract were measured for antioxidant capacity using standard α-tocopherol solution in a UV-Vis spectrophotometer at a maximum wavelength of 757 nm. The results showed that the sample of red fruit ethanol extract (Pandanus conoideus Lam.) Had total antioxidant activity with an activity of 1,392 x 10-3 g ATE / g extract.
Isolasi Bakteri Rhizosfer Tanaman Nilam (Pogostemon cablin Benth.) yang Berpotensi sebagai Penghasil Senyawa Antibakteri terhadap Bakteri Penyebab Infeksi Saluran Pencernaan Maulidiyah, Zakiya; Dali, Seniwati; Rusli, Rusli; Naid, Tadjuddin
Window of Health : Jurnal Kesehatan Vol. 3 No. 2 (April, 2020)
Publisher : Fakultas Kesehatan Masyarakat Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (245.974 KB) | DOI: 10.33368/woh.v0i0.295

Abstract

Research on isolation of rhizosphere bacteria in patchouli (Pogostemon cablin Benth.) Plants. This study aims to obtain patchouli plant rhizosphere bacteria which have the potential to produce antibacterial compounds against bacteria that cause digestive tract infections. The first stage, isolation of bacteria originating from the patchouli plant rhizosphere. Antibacterial screening test using a match method, obtained IBRN-3 and IBRN-5 isolates inhibited the growth of all test bacteria. IBRN-3 and IBRN-5 isolates were fermented. The fermentation results are extracted with ethyl acetate solvent to produce dry fermentate ethyl acetate extract. Thin Layer Chromatography was identified on the ethyl acetate extract of IBRN-3 and IBRN-5 using chloroform: methanol (8: 1) eluent and followed by TLC-Bioautography test. The results of spotting on IBRN-3 with Rf value 0.93 inhibited the bacteria E. coli, S. aureus, Rf value 0.82 inhibited B. subtillis, S. dysenteriae, Rf value 0.64 inhibited S. aureus and S. thypii, Rf value 0.89 , 0.36, 0.24 inhibited S. dysenteriae, Rf values ​​0.84 and 0.74 inhibited S. aureus. Whereas for IBRN-5, spots were obtained with an Rf value of 0.91 inhibiting B. subtillis, E. coli S. thypii, an Rf value of 0.95 inhibiting S. aureus S. dysenteriae, an Rf value of 0.84 inhibiting B. subtillis, S. thypii, Rf value of 0.83 inhibits S. dysenteriae, and Rf value of 0.74 inhibits S. thypii.
AKTIVITAS ANTIOKSIDAN FRAKSI RIMPANG KENCUR (KAEMPFERIA RHIZOMA) DENGAN MENGGUNAKAN METODE PEREDAMAN 1,1 DIPHENYL-2-PICRYLHYDRAZIL (DPPH) Muhafidzah, Zahrah; Dali, Seniwati; Syarif, Rezky Amriati
Jurnal Ilmiah As-Syifaa Vol 10, No 1 (2018): AS-SYIFAA Jurnal Farmasi
Publisher : Fakultas Farmasi UMI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (684.818 KB)

Abstract

Kaempferia is one of the most widely used plants in society is a traditional at the medicine. Empirically Kaempferia rhizome has a special quality as a wound healer, good for digestion, antibacterial, and as an antioksidant. Based on research been conducted that Kaempferia extract has antioxidant activity. This research aimed to test antioxidant activity base on IC50 value on kaempferia rhizome fraction by the reduction method 1,1 diphenil-2-phicrylhydrazil (DPPH). The extract obtained was fractionated using a vacuum liquid chromatography method, then performed free radical reduction meansurements using UV-VIS spectrophotometry. A positive fraction of antioxidants activity is the n-hexane fraction : ethyl acetate (7:3 and 8:2). The n-hexane fraction 8:2 showed higher antioxidant activity with IC50  731.832 µg/mL. The n-hexane fraction: ethyl acetate 7:3 with IC50  829.737 µg/mL
ISOLASI KITOSAN DARI LIMBAH CANGKANG KEPITING BAKAU (Scylla serrata) DAN APLIKASINYA TERHADAP PENYERAPAN TRIGLISERIDA Dali, Seniwati; Safitri, Nur Ramadhana Dewi; Fawwaz, Muammar
Jurnal Ilmiah As-Syifaa Vol 8, No 2 (2016): AS-SYIFAA Jurnal Farmasi
Publisher : Fakultas Farmasi UMI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (206.392 KB) | DOI: 10.33096/jifa.v8i2.200

Abstract

Chitosan is the result of deacetylation process from chitin which it can be found on Crustacean outer shell such as crabs. Chitosan can bind fat if it was consumed by human. The fat-binding ability of chitosan depends on the deacetylation degree. The research have been made into two phases. The first phase, chitosan was made from crab shell using NaOH. The deacetylation degree from chitosan that was made from the earlier process was analyzed with FTIR. The deacetylation degree result of the research was 59.39% using NaOH 50%. The second phase was the process of adsorbing triglycerides using chitosan in 10, 30, 45, and 60 minutes which was analyzed using spectrophotometer UV-Vis. The result of this research showed that the optimum triglycerides with 0.5, 1, 3 gram of chitosan was 2.99%, 3.14%, and 3.36%.
Isolation and Identification of Cellulolytic Bacteria from Gut of Horn Beetle Larvae (Oryctes rhinoceros L.) Riskawati, Riskawati; Natsir, Hasnah; Dali, Seniwati; Baharuddin, Maswati
Molekul Vol 18 No 2 (2023)
Publisher : Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jm.2023.18.2.6848

Abstract

The horn beetle larvae (Oryctes rhinoceros L.) contain symbiotic bacteria that are used to digest and degrade cellulose as food so that it has the potential to produce cellulase enzymes. This study aims to isolate, characterize and identify microbial symbionts from horn beetle larvae that have the potential to produce cellulase enzymes.The methods in this study include morphology and physiology identification of bacteria, qualitative and quantitative activity tests and species determination using 16S rRNA sequencing technique. Based on the results of morphological observations, five bacterial isolates were taken which has the potential as a cellulase producer is indicated by the presence of a clear zone that is produced when a qualitative test is carried out using congo red staining with different cellulolytic indices. Based on the quantitative bacterial activity test using UV-Vis, the highest activity was found in PES3 isolates at 1.62 x 10-2 and PES5 at 1.61 x 10-2. Species determination results found that PES3 isolates belonged to the genus Acinetobacter and PES5 belonged to Pseudomonas. In addition to the isolates obtained for the environment and the industrial sector, cellulolytic bacteria can provide added value such as hydrolyze cellulose waste into alternative fuels.
Isolation and Molecular Identification of Amylolytic Bacteria from Oryctes rhinoceros L. Larvae Decomposing Empty Palm Oil Fruit Bunches Uto, Sahriani; Arfah, Rugaiyah; Dali, Seniwati; Baharuddin, Maswati
Molekul Vol 18 No 2 (2023)
Publisher : Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jm.2023.18.2.6957

Abstract

Oryctes rhinoceros L. is an organism that helps the decomposition of oil palm empty fruit bunches (OPEFB). In the larvae's intestines, there are symbiotic bacteria that are used in the process of food degradation in the digestive system, one of which is amylolytic bacteria. This study aims to isolate and molecular identify amylolytic bacteria that produce amylase enzymes from horn beetle larvae. The techniques are used to screen and isolate bacteria from horn beetle larvae. Bacterial identification was accomplished by microscopically identifying amylase-producing bacterial isolates, performing biochemical tests on selected bacterial isolates, quantifying amylase enzyme activity, and molecularly identifying 16S rRNA. The results of screening and bacterial isolation obtained five isolates. The largest amylolytic bacterial colony index value was obtained in the EA3 isolate, which was 1.370 mm. Bacterial isolates with the highest activity were found in isolates coded EA1 and EA2, namely 0.049 U/mL and 0.0479 U/mL. According to the findings of 16S rRNA molecular identification, isolates EA1 and EA2 had similarities with the bacteria Ochrobactrum sp. and Pseudomonas mendocina.
The Degradation of Antracene Compound Using Bacteria Isolated From Paotere Port Waterways Mirnawati, Mirnawati; La Nafie, Nursiah; Dali, Seniwati
Indonesian Journal of Chemical Research Vol 3 No 1 (2015): Edisi Bulan Juli (Edition For July)
Publisher : Jurusan Kimia, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/ijcr.2015.3-mir

Abstract

The objectives of this study are (1) Determine the content of antrasena compound in the waste oil at the waterway of Paotere port (2) Determine the characteristics of antrasena degradation bacteria and (3) Determine the percentage of antrasena degradation. In this research an analysis of antrasena compound was conducted with sea water that had been polluted with oil. The next steps were to isolate and characterize the degrading bacteria of antrasena and to analyze the results of antrasena degradation. The results showed that (1) Concentration of the antrasena compound in the waterway of paotere port is 4,8x10-10 ppm. (2) The bacteria can degraded of the antracene is Bacillus cereus (3) There was evidence that Bacillus cereus was able to degrade antracenr compound, the highest degradation percentage is 98,11%, it was achieved on 12 day of incubation
Produksi Oligomer Kitosan dari Limbah Udang Windu (Panaeus monodon) Menggunakan Enzim Kitosanase dari Isolat Bakteri Klebsiella sp. Sarni, Sarni; Natsir, Hasnah; Dali, Seniwati
Indonesian Journal of Chemical Research Vol 3 No 2 (2016): Edisi Bulan Januari (Edition For January)
Publisher : Jurusan Kimia, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/ijcr.2016.3-sar

Abstract

Chitosan is a biopolymer that is the main content of D-glucosamine and several parts of N-acetyl-D-glucosamine bound to β- (1-4) glucoside. Chitosan receive special attention as functional biopolymers for applications in various fields. Chitosan is more effectively absorbed into the human body when it gets converted into chitosan oligomer form. Chitosan oligomer is a mixture of oligomers of D-glucosamine are formed through a process of severing ties depolymerization of chitosan with β-glycosidic. This study aims to produce chitosan oligomer of waste tiger shrimp (Penaeus monodon) using enzyme kitosanase of bacteria Klebsiella sp. Chitosan oligomer produced by using the enzyme chitosanase at a temperature of 40 °C and pH 8 with the activity of 0.309 U/mL (5,235 U/mg) obtained in the form of a mixture of monomer to octamer, which soluble in acetic acid 0.25% to 0.5%, having intrinsic viscosity decreases with increasing time of incubation is 0.195 (1 hour incubation); 0.9 (incubation 2 hours) and 0.7 (incubation 3 hours) with molecular weight range of 4103.12 g/mol (incubation 1 hour) ; 1483.48 g/mol (incubation 2 hours) and 1065.79 g/mol (incubation 3 hours).
Co-Authors A Amaliah Dahlia Abdul Wahid Wahab Amalia, Hilda Ayu Melvi Arfah, Rugaiyah Chairunnas, Armadi Dali, Arniah Firdaus Firdaus Harti Widiastuti Hasnah Natsir Irmawan, Muhammad Jayawarsa, A.A. Ketut Mandey, Frederyk Maswati Baharuddin Maulidiyah, Zakiya Muammar Fawwaz Muhafidzah, Zahrah Muhammad Zakir Nasriadi Dali Nasution, Annio Indah Lestari Nur Ramadhana Dewi Safitri, Nur Ramadhana Dewi NURAENI, ANDI Nursiah La Nafie Nursiah La Nafie Nuryadin, Yudhi Nuryadin, Yudhi Paulina Taba Permatasari, Nur Umriani Puspitasari, Sri Ayu Andini R. Rusli Riskawati Riskawati, Riskawati Rusman, Hendra J. Sarni Sarni Syaharuddin Kasim Syarif, Rezky Amriati Tadjuddin Naid Teheni, Muhammad T. Uto, Sahriani Wabula, Rheytno Asdin Wahyuni, Eka Sry Yusuf, Evana Y.