CYTOTOXICITY EXTRACT OF RED FRUIT (Pandanus Conoideus Lamk.) WITH BRINE SHRIMP LETHALITY TEST

Marliyana, Soerya D.
W, Fajar Rakhman
H, Nestri
Rakhmawati, Rita

Alchemy Jurnal Penelitian Kimia
Vol 8, No 1 (2012)

The cytotoxicity with Brine Shrimp Lethality Test (BSLT) extract of red fruit (Pandanus conoideus Lamk.) have been done. The ethanol and n-hexana extracts were evaluated for cytotoxicities. The n-hexane extract demonstrated higher cytotoxic activity than the ethanol extract, with 54% death in concentration 400 ?g/mL. The n-hexane extract was separated by column chromatography, eluting with a gradient of n-hexane-EtOAc to had an active fraction. The cytotoxicity of an active fraction showed with LC50 values of 138,05 µg/mL. Identification of this fraction exhibited three compounds of fatty acid were palmitic acid, oleic acid, and miristic acid