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All Journal Jurnal Ilmiah Farmasi The Journal of Pure and Applied Chemistry Research Alchemy Jurnal Penelitian Kimia Majalah Obat Tradisional Jurnal Pengabdian Masyarakat Bangsa
Rita Rakhmawati, Rita
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Agriculture, Biological Sciences & Forestry Arts Humanities Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Economics, Econometrics & Finance Education Energy Environmental Science Health Professions Industrial & Manufacturing Engineering Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Nursing Physics Public Health Social Sciences

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CYTOTOXICITY EXTRACT OF RED FRUIT (Pandanus Conoideus Lamk.) WITH BRINE SHRIMP LETHALITY TEST Marliyana, Soerya D.; W, Fajar Rakhman; H, Nestri; Rakhmawati, Rita
Alchemy Jurnal Penelitian Kimia Vol 8, No 1 (2012)
Publisher : Alchemy Jurnal Penelitian Kimia

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Abstract

The cytotoxicity with Brine Shrimp Lethality Test (BSLT) extract of red fruit (Pandanus conoideus Lamk.) have been done. The ethanol and n-hexana extracts were evaluated for cytotoxicities. The n-hexane extract demonstrated higher cytotoxic activity than the ethanol extract, with 54% death in concentration 400 ?g/mL. The n-hexane extract was separated by column chromatography, eluting with a gradient of n-hexane-EtOAc to had an active fraction. The cytotoxicity of an active fraction showed with LC50 values of 138,05 µg/mL. Identification of this fraction exhibited three compounds of fatty acid were palmitic acid, oleic acid, and miristic acid
UJI TOKSISITAS SECARA BRINE SHRIMP LETHALITY EKSTRAK BUAH MERAH (Pandanus Conoideus Lamk.) Marliyana, Soerya D.; Rakhman W, Fajar; Handayani, Nestri; Rakhmawati, Rita
Alchemy Jurnal Penelitian Kimia Vol 8, No 2 (2012)
Publisher : Alchemy Jurnal Penelitian Kimia

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Abstract

Telah dilakukan uji toksisitas secara Brine Shrimp Lethality Test (BSLT) ekstrak buah merah (Pandanus Conoideus Lamk.). Uji toksisitas dilakukan pada ekstrak etanol dan n-heksana. Hasil uji BSLT menunjukkan rata-rata prosentase kematian ekstrak n-heksan lebih tinggi dibandingkan dengan ekstrak etanol, sebesar 54 % diukur pada konsentrasi 400 (μg/mL). Ekstrak n-heksan selanjutnya dilakukan pemisahan secara kromatografi kolom dengan elusi secara gradien menggunakan pelarut campuran n-heksana-etil asetat untuk memperoleh fraksi aktif. Uji toksisitas secara BSLT menunjukkan fraksi aktif mempunyai nilai LC50 sebesar 138,05 μg/mL. Identifikasi fraksi aktif diperoleh 3 komponen senyawa asam lemak yaitu asam palmitat, asam oleat dan asam miristat.Kata Kunci : Buah merah (Pandanus conoideus Lamk.), identifikasi, toksisitas, Brine Shrimp LethalityABSTRACT
In vivo Wound Healing Activity of Tamanu Oil (Calophyllum inophyllum l.) Extracted by Cold-Pressed and Hot-Pressed Methods and Their Fatty Acids Composition Rakhmawati, Rita; Wahyuni, Dinar Sari Cahyaningrum; Ainurofiq, Ahmad; Hadi, Saptono; Choiri, Syaiful; Retnaningtyas, Estu; Pratama, Tiara Dewi Salindri
Majalah Obat Tradisional Vol 29, No 3 (2024)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/mot.97146

Abstract

Tamanu oil is a promising natural wound healer due to its chemical compounds, particularly fatty acids. Cold-pressed or hot-pressed methods are commonly used to obtain this oil from Calophyllum seed. However, there needs to be research documenting the impact of these two extraction methods on the fatty acid profile and wound healing activity. Therefore, the study aims to characterize the fatty acid profiles of cold-pressed and hot-pressed tamanu oils using Gas Chromatography and evaluate their wound healing activity in vivo. The fatty acid profiles were analyzed using GC, and the wound healing tests were conducted on animal subjects divided into four groups: negative control, positive control (Bioplacenton®), cold-pressed tamanu oil, and hot-pressed tamanu oil. Cold-pressed tamanu oil showed superior fatty acid characteristics with an acid value of 38,71 Mg KOH/g fat and a peroxide value of 3,0095 mEq O2/kg, indicating that oil is stable against oxidation.  The length of the wound was observed daily for up to 8 days to assess its effect. The parameters observed were the percentage of wound healing and the total AUC based on the average length of the wound. Cold-pressed tamanu oil demonstrated the highest wound healing efficacy compared to both the positive control and hot-pressed tamanu oils. Both cold (11,67± 0,78) and hot-pressed tamanu oil (11,87 ± 0,61) exhibited significant differences in AUC value compared to the negative control group (13,07 ± 0,38), highlighting the potential of tamanu oil as a wound healing agent.
Pemanfaatan Sumber Daya Alam Lokal dalam Peningkatan Produktivitas Industri Rumah Tangga di Ingasrejo, Gondangrejo, Kabupaten Karanganyar Ainurofiq, Ahmad; Hadi, Saptono; Rakhmawati, Rita; Choiri, Syaiful; Wahyuni, Dinar Sari C; Nugraheni, Estu Retnaningtyas; Pratama, Tiara Dewi Salindri
Jurnal Pengabdian Masyarakat Bangsa Vol. 2 No. 8 (2024): Oktober
Publisher : Amirul Bangun Bangsa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59837/jpmba.v2i8.1497

Abstract

Indonesia memiliki keanekaragaman hayati yang melimpah. Banyak tanaman di negara ini memiliki potensi besar untuk dimanfaatkan sebagai bahan baku produk bernilai ekonomi tinggi. Salah satunya adalah tanaman lerak (Sapindus rarak). Namun, tidak semua masyarakat mampu mengoptimalkan potensi tersebut karena kurangnya pengetahuan dan keterampilan mengenai pengolahan dan pemanfaatan bahan alam. Oleh karena itu, pengabdian ini ditujukan untuk memanfaatkan sumber daya alam lokal dalam meningkatkan produktivitas industri rumah tangga. Pengabdian ini dilakukan dengan metode sosialisasi, pendampingan, dan pelatihan untuk masyarakat Dusun Ingasrejo dalam mengoptimalkan buah lerak sebagai sabun cuci antiiritasi dan ramah lingkungan serta memilki nilai komersial. Dari hasil pengabdian didapatkan masyarakat memiliki pengetahuan lebih dan mendalam mengenai bahan alam khususnya buah lerak. Hal tersebut dibuktikan dengan adanya peningkatan pemahaman warga setelah dilakukan evaluasi, serta mengasah keterampilan baru yaitu pembuatan produk sabun cuci buah lerak. Selain itu juga memberikan manfaat ekonomi serta pelestarian lingkungan.
Standarisasi Proses Manufaktur Granul Jamu Instan Berbasis Kadar Fenolik Total, Aktivitas Antioksidan, dan Evaluasi Sifat Fisik Ainurofiq, Ahmad; Choiri, Syaiful; Hadi, Saptono; Wahyuni, Dinar Sari C.; Rakhmawati, Rita; Nugraheni, Estu Retnaningtyas; Pratama, Tiara Dewi S.; Azizah, Salma Nur
Jurnal Pengabdian Masyarakat Bangsa Vol. 2 No. 9 (2024): November
Publisher : Amirul Bangun Bangsa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59837/jpmba.v2i9.1551

Abstract

Jamu merupakan warisan budaya Indonesia yang telah digunakan secara turun menurun oleh nenek moyang. Penggunaan jamu dalam pengobatan hanya didasarkan pada data empiris yang diturunakan terus menerus antar generasi. Pembuktian khasiat dan kemanan jamu secara ilmiah telah diatur dalam Peraturan Menteri Kesehatan Republik Indonesia Nomor : 003/MENKES/PER/I/2010 dalam bentuk saintifikasi jamu. Saintifikasi produk jamu industri perlu dilakukan untuk mempertahankan eksistensi produk jamu di masyarakat. Tujuan pengabdian ini yaitu untuk meningkatkan penyediaan jamu berkhasiat melalui pembuktian khasiat jamu secara ilmiah. Standarisasi proses manufaktur granul jamu instan dilakukan sebagai bentuk saintifikasi jamu di industri. Standarisasi proses dilakukan berdasarkan penetapan kadar fenolik total dan nilai aktivitas antioksidan. Kadar fenolik total dianalisis dengan teknik Folin-Ciocalteu menggunakan asam galat sebagai senyawa pembanding. Aktivitas antioksidan dianalisis berdasarkan reaksi transfer electron senyawa antioksidan dengan •ABTS. Hasil penetapan menunjukkan adanya penurunan kadar fenolik total dari rimpang (0.1% GAE/g), hasil penyarian (0.065% GAE/g), dan hasil ruahan (0.057% GAE/g). Hasil penetapan aktivitas antioksidan menunjukkan adanya penurunan aktivitas antioksidan dari rimpang (29.79 IC50 mg/mL) ke hasil penyarian (34.62 IC50 mg/mL) serta terdapat peningkatan pada hasil ruahan (17.18 IC50 mg/mL) karena adanya bahan tambahan.
Review: Isolasi, Peningkatan Kadar, dan Analisis Calophyllolide dalam Minyak Calophyllum inophyllum Linn. serta Potensinya untuk Terapi Luka Bakar Herawati, Zainab; Rakhmawati, Rita
Jurnal Ilmiah Farmasi Vol. 21 No. 1 (2025): Jurnal Ilmiah Farmasi
Publisher : Universitas Islam Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20885/jif.vol21.iss1.art10

Abstract

Background: Calophyllolide is a dipyranocoumarin compound found in Calophyllum inophyllum L., known for its antimicrobial and anti-inflammatory properties, which are beneficial for burn wound healing. However, variability in its content and lack of standardized methods remain challenges.Objective: This review aims to present a literature study on calophyllolide, including its sources, isolation techniques, bioactive content optimization, analytical methods, and pharmacological potential in burn wound healing.Method: Data were retrieved from Scopus and PubMed using predefined keywords. Articles published in English between 2001 and 2021 and classified as original research were selected. Relevant studies were assessed for quality using the SYRCLE tool (animal studies) and the Young & Solomon checklist (non-clinical research).Results: Seeds harvested in September had the highest calophyllolide content (0.23%). Enhancement through tissue culture using 2 mg/L IBA yielded up to 45.23 mg/100 g callus. Among analytical techniques, a validated GC-MS method showed high precision and recovery. Pharmacological studies confirmed its activity against Staphylococcus aureus and its ability to modulate inflammatory responses.Conclusion: Calophyllolide shows strong potential as a natural agent for burn wound therapy. Standardized extraction, quantification, and production approaches are essential for further development.
Analyzing Curcumin Content using UV-Vis Spectrophotometry and TLC-Densitometry Methods: A Case Study in Curcuma Capsule Commercial Products Rakhmawati, Rita; Anastacia, Laura; Hadi, Saptono
The Journal of Pure and Applied Chemistry Research Vol. 14 No. 2 (2025): Edition May-August 2025
Publisher : Chemistry Department, The University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/

Abstract

Curcuma is a genus of plants commonly used in herbal medicine due to its benefits, primarily from the active compound curcumin. Accurate analytical methods are necessary to identify and quantify curcumin for quality control, especially in small-scale industries. This study compares spectrophotometry UV-visible and TLC-densitometry methods for analyzing Curcuma capsules from PT Rachmasari Group, a small-scale traditional medicine industry (UKOT) in Sukoharjo. The methods were evaluated based on linearity, LOD, LOQ, repeatability, intermediate precision, and accuracy. TLC-densitometry used a mobile phase of chloroform: methanol (95:5 v/v) and silica plates F254, while spectrophotometry used ethanol as the solvent. Both methods used a wavelength of 420 nm. Spectrophotometry showed better linearity (r2 = 0.9985) than TLC (r2 = 0.9847), while TLC-densitometry demonstrated higher sensitivity with lower LOD and LOQ values (1.2853 and 4.2845 μg/mL) compared to spectrophotometry (13.9261 and 46.4203 μg/mL). UV-Visible spectrophotometry exhibited superior repeatability (0.33 ± 0.02%) and intermediate precision (0.34 ± 0.01%) than TLC (1.86 ± 0.12% and 1.02 ± 0.88%). Accuracy ranged from 98.45–105.90% for spectrophotometry and 98.81–115.82% for TLC-densitometry. In conclusion, UV-Visible spectrophotometry is more precise, linear, and reliable overall, making it more suitable for curcumin quality control in small-scale herbal product industries.
Co-Authors Ahmad Ainurofiq, Ahmad Anastacia, Laura Azizah, Salma Nur Dinar Sari Cahyaningrum Wahyuni ESTU RETNANINGTYAS N Fajar Rakhman W, Fajar Fajar Rakhman W, Fajar Rakhman Herawati, Zainab Nestri H, Nestri Nestri Handayani, Nestri Pratama, Tiara Dewi S. Pratama, Tiara Dewi Salindri Saptono Hadi, Saptono Soerya Dewi Marliyana Syaiful Choiri Wahyuni, Dinar Sari C Wahyuni, Dinar Sari C.