Jurnal Penelitian Pendidikan IPA (JPPIPA)
Vol 10 No 4 (2024): April

Isolation and Characterization of Pathogenic Mold Causing Potato Tuber Rot Disease

Rizal Koen Asharo (Universitas Negeri Jakarta)
Reni Indrayanti (Unversitas Negeri Jakarta)
Azizatul Amala (Unversitas Negeri Jakarta)
Eldrian Daffa Raihan (Unversitas Negeri Jakarta)
Raymond Rayhand Tampanguma (Unversitas Negeri Jakarta)
Hilda Arsyah Eka Putri (Unversitas Negeri Jakarta)
Pinta Omas Pasaribu (Unversitas Negeri Jakarta)
Nurul Assyifa Wardana (Unversitas Negeri Jakarta)



Article Info

Publish Date
25 Apr 2024

Abstract

The potato plant (Solanum tuberosum L.) is a perennial crop that contains high amounts of carbohydrates, minerals, and vitamins in its tubers, making it a carbohydrate-rich alternative food to rice or corn. One of the diseases that often appear on potato plants is potato rot caused by pathogenic molds. This study aims to isolate and characterize pathogenic fungi that cause blight on potato tubers based on Koch's Postulates. Potato pathogenic molds were isolated from potato tubers that had been rotted, then the molds were grown on PDA and purified twice. The purified isolates were then inoculated onto 30 healthy potato tubers and incubated for 7 days. Healthy potato tubers experienced the same symptoms as potato tuber rot. The isolated pathogenic fungi were then characterized so that the pathogenic fungi of Phytophthora infestans were obtained. In this study, Koch's Postulate method was successfully applied to isolate and characterize the pathogenic fungi that cause potato tuber rot. The pathogenic fungi caused a potato tuber rot disease incidence value of 100% with a disease severity value of 60.7%. The two factors that determine the disease incidence and disease severity values are internal factors (genes and traits of the pathogen) and external factors (environment).

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Journal Info

Abbrev

jppipa

Publisher

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education Materials Science & Nanotechnology Physics

Description

Science Educational Research Journal is international open access, published by Science Master Program of Science Education Graduate Program University of Mataram, contains scientific articles both in the form of research results and literature review that includes science, technology and teaching ...