Subchronic Toxicity Test of Purple Leaves Ethanol Extract (PLEE) on the Histopathological Picture of the Lymph of Wistar Rats and Antioxidant Activity

Darmayanti (STIFA Pelita Mas Palu)
Niluh Puspita Dewi (STIFA Pelita Mas Palu)
Mariyani (STIFA Pelita Mas Palu)
Joni Tandi (STIFA Pelita Mas Palu)
Meike Rachmawati (Biopath Laboratory)
Yuliet Susanto (Tadulako University)

Jurnal Penelitian Pendidikan IPA (JPPIPA)
Vol 10 No 7 (2024): July

This study aims to determine the toxic effects of purple leaf ethanol extract on lymph organs at doses of 500; 2.000; and 5.000 mg/kgBB administered for 28 days and and to determine the antioxidant activity of purple leaf Graptophyllum pictum (L.) This study is an experimental study with a random group design, using 20 test animals divided into 4 groups consisting of two treatment groups, namely in the normal control group given Na-CMC 0.5% and the experimental group given PLEE (k1: dose 500 mg/kgBB, k2: dose 2.000 mg/kgBB and k3: dose 5.000 mg/kgBB hematoxylin-eosin staining (HE) using a computer-connected Motic BA210 microscope with 40X magnification in 5 fields of view and antioxidant test using DPPH method with UV-Vis Spectrophotometer at 516 nm wavelength with concentration 20, 40, 60, 80, and 100 ppm. The results showed that leaf ethanol extract was toxic at a dose of 2.000 mg/kgBB – 5.000 mg/kgBB against the diameter of lymphatic organ pulp, organ index and organ weight, there were symptoms of toxicity and purple leaf ethanol extract had strong antioxidant activity with 66.26 μg/mL.