Lactiplantibacillus plantarum IS-10506 Enhances Tight Junction Integrity in Bronchial Epithelium: An Experimental Study

Deasy Fetarayani (Doctoral Program of Medical Science, Faculty of Medicine, Universitas Airlangga, Jl. Prof. Dr. Moestopo No. 47, Surabaya 60132)
Gatot Soegiarto (2Department of Internal Medicine, Faculty of Medicine, Universitas Airlangga/Dr. Soetomo General Academic Hospital, Jl. Prof. Dr. Moestopo No. 47, Surabaya 60132)
Ingrid Suryanti Surono (Food Technology Department, Faculty of Engineering, Bina Nusantara University, Jl. K.H. Syahdan No. 9, Jakarta 11480)
Anang Endaryanto (Department of Child Health, Faculty of Medicine, Universitas Airlangga/Dr. Soetomo General Academic Hospital, Jl. Prof. Dr. Moestopo No. 47, Surabaya 60132)
Alpha Fardah Athiyyah (Department of Child Health, Faculty of Medicine, Universitas Airlangga/Dr. Soetomo General Academic Hospital, Jl. Prof. Dr. Moestopo No. 47, Surabaya 60132)
Yetti Hernaningsih (Department of Clinical Pathology, Faculty of Medicine, Universitas Airlangga/Dr. Soetomo General Academic Hospital, Jl. Prof. Dr. Moestopo No. 47, Surabaya 60132)
Dyah Fauziah (Department of Anatomical Pathology, Faculty of Medicine, Universitas Airlangga/Dr. Soetomo General Academic Hospital, Jl. Prof. Dr. Moestopo No. 47, Surabaya 60132)
I Ketut Sudiana (Department of Anatomical Pathology, Faculty of Medicine, Universitas Airlangga/Dr. Soetomo General Academic Hospital, Jl. Prof. Dr. Moestopo No. 47, Surabaya 60132)
Budi Utomo (Department of Public Health and Preventive Medicine, Faculty of Medicine, Universitas Airlangga, Jl. Prof. Dr. Moestopo No. 47, Surabaya 60132)
Cita Rosita Sigit Prakoeswa (8Department of Dermatology and Venereology, Faculty of Medicine, Universitas Airlangga/Dr. Soetomo General Academic Hospital, Jl. Prof. Dr. Moestopo No. 47, Surabaya 60132)

The Indonesian Biomedical Journal
Vol 16, No 2 (2024)

BACKGROUND: Airway epithelium constitutes the initial structural defense against inhaled allergens, with its integrity primarily maintained through the formation of tight junctions (TJ) consisting of claudins, occludins, and zonula occludens (ZO). Currently, the effects of probiotics on airway TJ remain unclear. Therefore, this study was conducted to explore the impact of Lactiplantibacillus plantarum IS-10506, a native probiotic from Indonesia, on bronchial epithelial TJ in a mouse model of allergic airway inflammation.METHODS: Twenty-four BALB/c mice were randomly assigned to three groups: negative control, positive control subjected to Dermatophagoides pteryonissinus (Der p) sensitization and challenge, and treatment group exposed to Der p and administered L. plantarum IS-10506 since one week before sensitization up to three weeks after. The expression levels of claudin-18, occludin, and ZO-1 were investigated through immunohistochemistry analysis and quantified based on the immunoreactive score (IRS). RESULTS: The results of this study demonstrated that L. plantarum IS-10506 significantly enhanced the expressions of claudin-18, occludin, and ZO-1 (p<0.05).CONCLUSION: These findings underscore the probiotic's role in fortifying the integrity of airway TJ.KEYWORDS: allergic inflammation, probiotic Lactiplantibacillus plantarum, immunology, respiratory system, tight junction, occludin, claudin, zonula occludens