This study aims to analyze the differences in vitamin D3 levels in fish oil in the body parts of lamb fish. The method used is an experiment with a Factorial Randomized Design with three replications. The results showed that the Vitamin D3 content in lamb fish oil is quite high, ranging from 767.52 IU / g to 787.34 IU / g. The highest vitamin D3 content is in group one, namely the whole fish (scales, head, meat, bones, and innards). The conclusion is that there is a significant difference between the vitamin D3 content in fish oil and whole fish, meat, meat, and waste. Keywords: Inflammation, Fish Oil, Flat Lambak, Vitamin D
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