Penilaian densitas kolagen pada luka bakar derajat II tikus putih (Rattus norvegicus) yang diterapi dengan krim ekstrak daun melinjo (Gnetum gnemon L.)

Leonardo Reza Srimaryanto (Master of Disease and Veterinary Public Health, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, East Java, Indonesia.)
Nove Hidajati (Division of Basic Veterinary Medicine, Faculty of Veterinary Medicine, Universitas Airlangga, Airlangga, Surabaya, East Java, Indonesia.)
Nusdianto Triakoso (Division of Veterinary Clinic, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, East Java, Indonesia.)
Eduardus Bimo Aksono Herupradoto (Division of Basic Veterinary Medicine, Faculty of Veterinary Medicine, Universitas Airlangga, Airlangga, Surabaya, East Java, Indonesia.)
Mochamad Lazuardi (Division of Basic Veterinary Medicine, Faculty of Veterinary Medicine, Universitas Airlangga, Airlangga, Surabaya, East Java, Indonesia.)
Boedi Setiawan (Division of Veterinary Clinic, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, East Java, Indonesia.)

Journal of Pharmaceutical and Sciences.
JPS Volume 8 Nomor 1 (2025)

Second-degree burns are complex traumatic conditions requiring optimal tissue healing interventions. This experimental study investigated the impact of Gnetum gnemon L. leaf extract cream on collagen density in burn wounds. The research was conducted using 20 male Wistar rats (Rattus norvegicus), divided into five groups: negative control, positive control (silver sulfadiazine), and three treatment groups with varying leaf extract concentrations (2.5%, 5%, and 10%). Burn induction was performed on the right gluteal area using a thermostat at 85°C for 5 seconds. Collagen density was evaluated through histopathological analysis using ImageJ with density scores ranging from 0 to 4. Research results demonstrated significant collagen density increases in treatment groups, with the 10% concentration displaying the highest density (score +4). Significant differences between groups were confirmed by statistical analysis (p<0.05). Findings indicate the potential of Gnetum gnemon L. extract as an alternative therapy for improving burn wound healing through collagen density optimization.