This study aimed to evaluate the effect of different extraction methods on the quality of alginate derived from Turbinaria murayana seaweed as a poultry feed additive. An experimental approach was employed using a completely randomised design (CRD) consisting of three treatments and six replications. The treatments involved three different extraction methods: acid, alkaline, and calcium pathways. The data were analysed using analysis of variance (ANOVA), and significant differences among treatments were further examined using Duncan’s Multiple Range Test. The results indicated that the extraction method had a highly significant effect (P<0.01) on alginate dry matter content and purity, while it had no significant effect (P>0.05) on alginate pH. Among the tested methods, the acid extraction pathway produced the highest quality alginate, meeting the quality standards set by the Food Chemicals Codex (FCC, 2004), with a dry matter content of 86.57%, a pH value of 9.17, and alginate purity of 36.28%.
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