<![CDATA[This study aims to determine the effect of different extraction solvents (n-hexane, ethyl acetate, and ethanol) on the antioxidant activity of mustard green (Brassica juncea L.) extracts. The extraction was carried out using the maceration method with different solvent polarities, and the concentrated extracts were tested for phytochemical screening and antioxidant activity using the DPPH method. The IC₅₀ values were analyzed using one-way ANOVA, resulting in a significance value of 0.000 (p < 0.05), indicating that the type of solvent significantly affected antioxidant activity. Phytochemical analysis revealed the presence of flavonoids, phenols, tannins, saponins, alkaloids, and steroids. The average IC₅₀ values of n-hexane, ethyl acetate, and ethanol extracts were 314.73±4.96 ppm (weak), 412.29±3.06 ppm (weak), and 40.46±0.63 ppm (very strong), respectively. These results indicate that solvent polarity variations significantly influence the antioxidant activity of mustard green extracts.]]>
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