Synthesis and Cytotoxic Activity of Methoxylated Chalcones in Breast Cancer MCF-7 and Prostate Cancer DU-145 Cell Lines

Fareza, Muhamad Salman
Samudra, Genta Hafied Naga
Asrada, Syahdan
Fischellya, Dafi
Wijaya, Triyadi Hendra
Choironi, Nur Amalia
Wasito, Hendri
Suhesti, Tuti Sri
Mustikaningtyas, Ika
Rehana, Rehana
Setiyabudi, Lulu
Sarmoko, Sarmoko

Molekul: Jurnal Ilmiah Kimia
Vol 20 No 3 (2025)

Chalcones, a class of naturally occurring compounds, exhibit a broad spectrum of biological activities, including anticancer properties. In this study, a series of methoxylated chalcones were synthesized via Claisen-Schmidt condensation and evaluated for cytotoxic activity against breast cancer MCF-7 and prostate cancer DU-145 cell lines. The synthetic route involved Claisen-Schmidt condensation, leading to various methoxy-substituted chalcone derivatives. The structures of the synthesized chalcones were confirmed through NMR and mass spectrometry. Cytotoxicity was assessed using the PrestoBlue assay, with 4-bromochalcone (compound 2) displaying the highest cytotoxic activity against MCF-7 cancer cell lines (IC50 = 26.99 µM). These results indicate that methoxylated chalcones hold promise as potential lead compounds for the development of new anticancer agents targeting breast and prostate cancer.