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All Journal Journal Of Agriculture Sciences (Agrosci)
Neli Pratiwi, Dian
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Agriculture, Biological Sciences & Forestry

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DNA Detection Sensitivity Test from Blood Sample Extraction of Sumatran Elephant (Elephas Maximus Sumatranus) Male at Elephant Training Center, Way Kambas National Park Using Simple and Molecular Methods Neli Pratiwi, Dian; Priyambodo, Priyambodo; Angeliya, Liza; Agus Srihanto, Eko; Candra, Dedi; Devi Krismuniarti, Elisabeth; Lestari Rustitati, Elly
Jurnal Agrosci Vol 1 No 2 (2023): Vol 1 No 2 November 2023
Publisher : Ann Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62885/agrosci.v1i2.109

Abstract

DNA detection of male sumatran elephants' (Elephas maximus sumatranus) blood samples using simple and molecular methods are ways to find out the DNA quality from extracted samples. Molecular biology analysis was done in Biotechnology Laboratory, Lampung Disease Investigation Center, under the Institution Nation Strategy Grant, Directorate General of Research Empowerment and Development, Ministri of Research, Technology and Higher Education 2018 Number 393/UN26.21/PN/2018. Sensitivity test on 12 blood samples was conducted using simple and molecular methods. Simple method is based on 1% gel agarose electrophoresis in TAE (Tris-acetate EDTA) buffer while molecular method involves Polymerase Chain Reaction (PCR) based Glyseraldehyde-3-Phosphate Dehydrogenase (GAPDH) primer. Of 12 samples extracted and tested, 10 samples using molecular method showed DNA bands. Sensitivity value (83.33%) of diagnostic calculation showed that molecular method is more sensitive in detecting DNA.
Sex Determination in Racing Pigeons (Columba livia) Molecularly Using Young Feather Samples Adelia, Ersa Imelda; Srihanto, Eko Agus; Rustiati, Elly Lestari; Master, Jani; Firwantoni, Firwantoni; Anggy, Fransiska Panasea; Neli Pratiwi, Dian
Jurnal Agrosci Vol 2 No 6 (2025): Vol 2 No 6 July 2025
Publisher : Ann Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62885/agrosci.v2i6.807

Abstract

The racing pigeon (Columba livia) has an aesthetic value and is in high demand. Pigeon is monomorphic, difficult to distinguish between male and female individuals, especially at the age of offspring. Knowing the sex of birds from an early age is necessary to prepare male bird individual as racing bird. Accurate determination of the sex of racing pigeons from an early age was carried out to have scientific confirmation of the molecular method of determining the sex in racing pigeon. Under Lampung Disease Investigation Center program, molecular methods of PCR techniques using primers 2550F and 2718R were applied to determine the sex of bird based on the presence of Z and W chromosomes from young feathers of racing pigeons. Sampling young feathers was done in individual breeders in Kota Metro and East Lampung. Molecular analysis was carried out at the Biotechnology Laboratory of the Lampung Disease Investigation Center, consisted of extraction, amplification, electrophoresis, and data analysis. Fifteen young feather samples showed five male individuals with electrophoresis visualization formed one band measuring 600 bp and ten female individuals showed two bands measuring 600 bp and 400 bp.
DNA Extraction of Cynopterus brachyotis of Labuhan Ratu VII Based on Column Method in Supporting its Molecular Species Confirmation Maharani, Annisa Lidya; Rustiati, Elly Lestari; Priyambodo, Priyambodo; Srihanto, Eko Agus; Saswiyanti, Enny; Neli Pratiwi, Dian; Susanto, Alvin Wiwiet
Jurnal Agrosci Vol 3 No 1 (2025): Vol 3 No 1 September 2025
Publisher : Ann Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62885/agrosci.v3i1.1043

Abstract

Background. Bats (Chiroptera) are true flying mammals and nocturnal. Ecologically, bats play important roles as pollinators, seed dispersers, guano producers, biocontrol, and hosts a range of viruses. Cynopterus brachyotis belongs to fruit-eating bats and is considered morphologically cryptic with C. sphinx. Aims. To support morphological identification, molecular species confirmation needed to be conducted. DNA extraction, as an initial step, plays a crucial role in molecular analysis. Methods. The quality and quantity of extracted DNA determine the success rate of sequencing for species confirmation in bats. In this study, oropharyngeal samples were used. Result. The research procedures included bat capture and DNA oropharyngeal swab sample collection, sample preparation, DNA extraction using a silica column-based commercial kit, DNA concentration measurement using a Qubit assay, and electrophoresis. Conclusion. The Qubit assay showed DNA concentrations of >1 ng/µL, while electrophoresis did not reveal bright DNA bands.
Co-Authors Adelia, Ersa Imelda Agus Srihanto, Eko Anggy, Fransiska Panasea DEDI CANDRA Devi Krismuniarti, Elisabeth Eko Agus Srihanto, Eko Agus Elly Lestari Rustiati Firwantoni, Firwantoni Jani Master, Jani Lestari Rustitati, Elly Liza Angeliya Maharani, Annisa Lidya Priyambodo Priyambodo Saswiyanti, Enny Susanto, Alvin Wiwiet