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All Journal Biospecies El-Hayah : Jurnal Biologi Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Biosfer: Jurnal Tadris Biologi Jurnal Biologi Tropis BIOVALENTIA: Biological Research Journal JUSTICIA SAINS: Jurnal Ilmu Hukum Organisms: Journal of Biosciences Journal Of Medicine And Health Sciences (Medisci)
Saswiyanti, Enny
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Environmental Science Health Professions Immunology & microbiology Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Nursing Public Health Social Sciences Veterinary Other

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THE ESSENTIAL CONTRIBUTION OF CAPTIVE SUMATRAN ELEPHANT IN ELEPHANT TRAINING CENTER, WAY KAMBAS NATIONAL PARK FOR WILDLIFE GENETICS CONSERVATION Rustiati, Elly Lestari; Priyambodo, Priyambodo; Yulianti, Yanti; Srihanto, Eko Agus; Pratiwi, Dian Neli; Virnarenata, Elsa; Novianasari, Tika; Krismuniarti, Elisabeth Devi; Saswiyanti, Enny
BIOVALENTIA: Biological Research Journal Vol. 6 No. 1 (2020)
Publisher : Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1061.173 KB) | DOI: 10.24233/BIOV.6.1.2020.173

Abstract

Way Kambas National Park (WKNP) is home of five protected big mammals including sumatran elephants.  It shares its border with 22 of 37 villages surrounding the national park.  Understanding their existence in the wild is a priority, and  wildlife genetics is a crucially needed. Besides poaching and habitat fragmentation, wildlife-human conflict is one big issue.  Elephant Training Center (ETC) in WKNP is built for semi in-situ conservation effort on captive sumatran elephants that mainly have conflict histories with local people.  Participative observation and bio-molecular analysis were conducted to learn the importance of captive Sumatran elephant for conservation effort.  Through captive sumatran elephants, database and applicable methods are expected to be developed supporting the conservation of their population in the wild.  Participative observation and molecular identification was carried on captive sumatran elephants in ETC, WKNP under multiple year Terapan grant of Ministry of Research and Technology Higher Education, Indonesia. Gene sequence and cytological analyses showed that the captive sumatran elephants are closely related and tend to be domesticated.  Translocation among ETC to avoid inbreeding, and maintaining the captive sumatran elephant as natural as possible are highly recommended. Developing genetic database can be a reference for both captive and wild sumatran elephants.
Identification of Visual Character of Leaves with Sumatran Rhino's Urine in the Sumatran Rhino Sanctuary Way Kambas National Park as Potential Source of eDNA Priyambodo, Priyambodo; Putri, Chicka R.P.; Rustiati, Elly L.; Kurniawati, Yeyen; Zulkarnain, Danisworo; Pratiwi, Dian N.; Arsan, Zulfi; Giyono, Giyono; Mustikawati, Ganis; Pertiwi, Vindo R.; Sukatmoko, Sukatmoko; Srihanto, Eko A.; Saswiyanti, Enny
El-Hayah:Jurnal Biologi Vol 9, No 2 (2023): EL-HAYAH (VOL 9, NO 2 Maret 2023)
Publisher : Program Study of Biology, Science and Technology Faculty, UIN Maulana Malik Ibrahim Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18860/elha.v9i2.21179

Abstract

The sumatran rhinoceros is one of the large endemic mammals to the island of Sumatra, with critically endangered status by the IUCN. Currently, the existence of the sumatran rhino is challenging to find in its natural habitat. Therefore, it is necessary to develop indirect monitoring to maintain and increase the stability of the population in nature. Monitoring through camera traps has been used in several locations. Another method is carried out by identifying traces and remains of the sumatran rhino in natural habitats, for example, through urine samples. The sumatran rhinoceros has the behavior of urinating, namely through urine spray. Urine sprayed by the sumatran rhino will hit plant parts in its habitat, including leaves. This study was aimed to identify the visual characteristics of leaves containing samples of sumatran rhino urination as a potential source of eDNA material. The results showed the leaves characteristics containing the sumatran rhino's urine on the 0, 1, and 2 days after the sumatran rhino urination. The difference in visual observations of the sumatran rhino's urine spray attached to the leaves can be seen through the change in the color of the urine fluid, which is getting more and more concentrated. Visualization of leaves containing sumatran rhino urine can be used to recommend visual leaf characteristics that can be taken as similar samples in natural habitats.
Undang-Undang Peternakan dan Kesehatan Hewan: Efektifitas dalam Pengendalian Penyakit Hewan Menular di Provinsi Lampung Saswiyanti, Enny; Wulandari, Christine; Bakri, Samsul
JUSTICIA SAINS - Jurnal Ilmu Hukum Vol 9, No 2 (2024): JUSTICIA SAINS: Jurnal Ilmu Hukum
Publisher : Universitas Sang Bumi Ruwa Jurai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24967/jcs.v9i2.3620

Abstract

Infectious animal diseases pose a serious threat to the health of animals and humans, as well as significant economic impacts. Law No. 41 of 2014 amending Law No. 19 of 2009 concerning Animal Husbandry and Animal Health regulates the security, assurance, utilization, and preservation of animals to ensure food sovereignty, independence, and security in order to achieve food sovereignty, independence, and security for the welfare and prosperity of the Indonesian people. The aim of this study is to analyze the effectiveness of the implementation of the Animal Husbandry and Animal Health Law and its derivatives in controlling infectious animal diseases in Lampung Province. The analysis results show that the implementation of the Animal Husbandry and Animal Health Law and its regulations has not been effective in protecting animal health, preventing disease, and its supervision processes have not been effective in preventing environmental contamination from the spread of infectious animal diseases in Lampung Province. This is evident from the entry and spread of animal diseases in Lampung Province such as Jembrana Disease, Foot and Mouth Disease (FMD), Lumpy Skin Disease (LSD), and African Swine Fever (ASF) in pigs
Phylogenetic Construction of Stingless Bees in Bandar Lampung based on 16S rRNA Gene Priyambodo, Priyambodo; Rustiati, Elly Lestari; Pratiwi, Dian Neli; Susanto, Alvin Wiwiet; Srihanto, Eko Agus; Saswiyanti, Enny
Biosfer: Jurnal Tadris Biologi Vol 15 No 2 (2024): Biosfer: Jurnal Tadris Biologi
Publisher : UNIVERSITAS ISLAM NEGERI RADEN INTAN LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24042/biosfer.v15i2.24589

Abstract

Stingless bees are one of the honey-producing groups that are currently in increasing demand. The significant rise in honey demand necessitates research into the variety of stingless bee species that have the potential to produce high-quality honey in large quantities. This study aims to determine the genetic relationship of stingless bees from two colonies in Rajabasa, Bandar Lampung. Samples were collected from two different colonies in Rajabasa, Bandar Lampung. Molecular analysis was conducted, including DNA extraction, amplification, DNA sequencing, and phylogenetic analysis using the 16S rRNA gene. The study assessed homology values, genetic distance, and the phylogenetic map. The results indicated that the individual samples, GB1 and GB2, found in Bandar Lampung, were closely related to the species Heterotrigona itama. This was confirmed by the query value of both samples being 100%, with similarity values of 96.99% and 97.20%, respectively. The genetic distance analysis, visualized in the phylogenetic map, also shows that the two samples are close relatives of Heterotrigona itama.ABSTRAK: Lebah tanpa sengat menjadi salah satu kelompok penghasil madu yang saat ini makin banyak diminati masyarakat. Peningkatan kebutuhan madu yang signifikan, memerlukan penelitian tentang ragam spesies lebah tanpa sengat yang potensial dalam menghasilkan madu yang berkualitas prima dalam jumlah yang melimpah. Penelitian ini telah dilakukan dengan tujuan untuk mengetahui kedekatan genetik lebah tanpa sengat dari dua koloni di Rajabasa, Bandar Lampung. Sampel diambil dari dua koloni yang berbeda di Rajabasa, Bandar Lampung. Analisis molekuler telah dilakukan dengan tahapan ekstraksi DNA, amplifikasi DNA, sekuensing DNA, dan dilanjutkan dengan analisis filogenetik dengan menggunakan gen 16S rRNA. Analisis dilakukan untuk mengetahui nilai homologi, jarak genetik, dan peta filogenetik. Hasil menunjukkan bahwa individu sampel GB1 dan GB2 yang ditemukan di Bandar Lampung mempunyai kedekatan dengan spesies Heterotrigona itama. Hal ini ditunjukkan dengan nilai query keduanya sebesar 100% dan nilai similarity masing-masing adalah 96,99% dan 97,20%. Analisis jarak geneti yang divisualisasi dalam peta filogenetik juga menunjukkan bahwa kedua sampel yang diperoleh merupakan kerabat dekat dari Heterotrigona itama.
Qualitative Analysis of Partial 16S rRNA Amplicon of Mitochondrial Gene of Stingless Bees in Pesawaran Priyambodo, Priyambodo; Rustiati, Elly Lestari; Pratiwi, Dian Neli; Susanto, Alvin Wiwiet; Imtitsal, Aulia; Fahrezi, Aditya; Febriansyah, Muhammad; Kusuma, Andriyani Wijaya; Srihanto, Eko Agus; Saswiyanti, Enny; Sidik, Mahfud; Sa'uddah, Lousanja D.; Lestari, Indah Ayu; Yani, Ani Andri; Ramadhan, Viki
Jurnal Biologi Tropis Vol. 23 No. 1 (2023): Special Issue
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v23i1.6470

Abstract

Pesawaran is one of the areas that has the potential to cultivate stingless bees as producers of honey and propolis. This study was aimed at qualitatively analysing partial amplicons of the 16S rRNA gene from individuals found at the research site. The research was performed from April to September 2023 in an explorative approach. Based on the exploration that has been done in Harapan Jaya Village, Way Ratai District, Pesawaran Regency, individual stingless bees were obtained from 5 different colonies. Individual samples of stingless bees were further extracted and amplified by commercial kits. Each individual from 5 different colonies has successfully obtained its DNA amplicons. Based on qualitative analysis using 1% agarose gel, the size of the partial gene ranged from 400bp to 500bp. This result is in accordance with the data contained in the genbank, where the 16S rRNA gene size is more than 500bp. Therefore, it has been concluded that the size of the partial amplicons of the 16S rRNA gene of individual stingless bees from the 5 colonies that were obtained is actually in range of the size of the 16S rRNA gene of stingless bees that have been recorded in the genbank.
Konstruksi Filogenetik Spesies Lebah Tanpa Sengat di Lampung Timur Berdasarkan Gen 16S rRNA Imtitsal, Aulia; Priyambodo; Rustiati, Elly Lestari; Pratiwi, Dian Neli; Susanto, Alvin Wiwiet; Ramadhan, Viki; Fahrezi, Aditya; Febriansyah, Muhammad; Kusuma, Andriyani Wijaya; Srihanto, Eko Agus; Saswiyanti, Enny
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 10, No 3 (2025): October 2025
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v10i3.9506

Abstract

Keanekaragaman flora dan fauna Indonesia sangat beragam. Jenis fauna yang terkenal di Indonesia adalah lebah tanpa sengat yang tersebar di seluruh wilayah di Indonesia. Informasi keragaman lebah tanpa sengat dan hubungan kekerabatan masing-masing kelompok lebah akan memberikan informasi terkait data populasi. Analisis molekuler berguna sebagai ilmu dan alat untuk melakukan identifikasi spesies. Analisis molekuler keragaman lebah tanpa sengat dapat menggunakan DNA mitokondria (mtDNA) pada lebah. Salah satu gen pengkode DNA mitokondria adalah gen 16S rRNA. Penelitian ini bertujuan untuk menganalisis sekuen sampel lebah tanpa sengat secara molekuler dan menyusun peta kekerabatan sehingga dapat menjadi data genetik dan informasi keanekaragaman spesie lebah tanpa sengat di Kabupaten Lampung Timur. Metode yang digunakan adalah  isolasi dan amplifikasi gen DNA, elektroforesis dan visualisasi, sekuensing dan analisis hasil sekuensing  menggunakan Basic Local Alignment Search Tool (BLAST), dan  konstruksi pohon filogenetik menggunakan Molecular Evolution Genetic Analysis (MEGA11). Berdasarkan penelitian ini 3 sampel lebah tanpa sengat yang didapatkan dari eksplorasi di Kabupaten Lampung Timur terdeteksi sebagai spesies Heterotrigona itama dan memiliki kekerabatan yang dekat dengan spesies sundatrigona sp dan paling jauh dengan Paratrigona lineatifrons. Hal ini sesuai dengan hasil uji BLAST dan analisis data molekuler menggunakan software Mega 11.
Learning Bats Handling For Oral Swab Sampling Azmi, Sevira Nur; Rustiati, Elly Lestari; Priyambodo, Priyambodo; Saswiyanti, Enny; Srihanto, Eko Agus; Pratiwi, Dian Neli; Susandi, Waryoko
Jurnal Medisci Vol 3 No 1 (2025): Vol 3 No 1 August 2025
Publisher : Ann Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62885/medisci.v3i1.1014

Abstract

Background. The only flying mammals, bats, play an essential role in their natural habitat. Based on the type of food, bats can be divided into two groups: fruit-eating bats and insect-eating bats. Bats rank second-highest in species diversity, with 1,439 worldwide and 239 in Indonesia. Known as reservoirs of viruses, including coronaviruses. Aims. Bats are suspected to be related to COVID-19. Under the Research Innovation and Collaboration Program - Higher Education for Technology and Innovation Project (HETI) University of Lampung 2024-2025, and in collaboration with the Lampung Disease Investigation Centre, learning the procedures for handling bats properly so as not to physically harm bats in oral swab sampling and species recognition in Braja Harjosari, directly next to Way Kambas National Park was done. Methods. The life-trapping technique uses a mist net. Bat handling for taking oral swab was carried out using the pinch grip method, which is holding both arms of the bat backwards utilizing the thumb and middle finger with the bat positioned facing upwards. Conclusion. The bat's mouth is then blown open and a cotton swab is gently inserted into the bat's mouth. Afterwards, the bat is rested, given water drops, and released into nature. Oral swab samples from 10 individual bats: frugivorous bats, Cynopterus brachyotis (n = 8), Cynopterus horsfieldii (n = 1), and insectivorous bat, Scotophilus kuhlii (n = 1).
Detection of Environmental DNA from Tree Bark Associated with Rubbing Behavior of Dicerorhinus sumatrensis in Way Kambas National Park Priyambodo; Zulkarnain, Danisworo; Rustiati, Elly Lestari; Kurniawati, Yeyen; Putri, Chicka Refina Rahma; Srihanto, Eko Agus; Saswiyanti, Enny; Arsan, Zulfi; Sukatmoko; Pratiwi, Dian Neli; Permatasari, Nindy
ORGANISMS: JOURNAL OF BIOSCIENCES Vol. 6 No. 1 (2026): Organisms: Journal of Biosciences
Publisher : Pusat HKI, Paten, dan Publikasi Ilmiah Universitas Islam Negeri Raden Intan Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24042/zv2m2645

Abstract

The development of non-invasive methods is essential for monitoring critically endangered species such as the Dicerorhinus sumatrensis. This study aimed to evaluate the potential of tree bark associated with rubbing behavior as a source of environmental DNA (eDNA) for species detection in Way Kambas National Park. A total of 30 samples were collected from tree bark surfaces showing evidence of rubbing activity. DNA was extracted and analyzed using two approaches: simple electrophoresis and polymerase chain reaction (PCR)-based molecular methods. The results showed that the simple method failed to detect visible DNA bands, indicating low DNA concentration and degradation in environmental samples. In contrast, PCR amplification successfully detected DNA in 5 out of 30 samples, including hair-derived and epithelial residues associated with rubbing behavior.containing hair residues produced more reliable detection compared to those with only epithelial traces. These findings demonstrate that tree bark able to serve as a potential non-invasive source of eDNA, particularly when analyzed using sensitive molecular techniques. This approach provides an alternative method for detecting elusive species and contributes to the development of effective conservation strategies for the sumatran rhinoceros.
PERBEDAAN LAMANYA HIDUP Trypanosoma evansi PADA BERBAGAI MEDIA Lusita, Lulu; Kanedi, M.; Saswiyanti, Enny; Setyaningrum, Endah; Valinata, Sisca
Biospecies Vol. 19 No. 2 (2026): Juli 2026
Publisher : Universitas Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22437/biospecies.v19i2.49174

Abstract

The diagnosis of Surra disease has limitations that make it impossible to test Trypanosoma evansi samples directly on all samples. This is due to limitations in time and medical and paramedical personnel in field diagnosis, thus requiring research on T. evansi live media solutions. The purpose of this study was to determine the lifespan of T. evansi in various media solutions and which media solution was most effective. This study was experimental in nature, using a Completely Randomized Design (CRD) and three replicates. Data analysis was performed using the LDS test, followed by an Analysis of Variance (ANOVA) test to find significant differences in the lifespan of T. evansi in various media solutions; Phosphate Buffer Saline Glucose (PBSG), Dulbeccos Modified Eagle Medium (DMEM) 1X + GlutaMAXTM, NaCl infusion (sodium chloride 0.9%), coconut water. The results of the statistical test at room temperature showed significant differences, with the Phosphate Buffer Saline Glucose (PBSG) solution sample being the best media solution for the survival of T. evansi parasites.
Co-Authors Arsan, Zulfi Azmi, Sevira Nur Christine Wulandari Eko Agus Srihanto, Eko Agus Elly Lestari Rustiati Endah Setyaningrum, Endah Fahrezi, Aditya Febriansyah, Muhammad Giyono Giyono Imtitsal, Aulia Indah Ayu Lestari, Indah Ayu Kanedi, M. Krismuniarti, Elisabeth Devi Kurniawati, Yeyen Kusuma, Andriyani Wijaya Lusita, Lulu Mustikawati, Ganis Novianasari, Tika Permatasari, Nindy Pertiwi, Vindo R. Pratiwi, Dian N. Pratiwi, Dian Neli Priyambodo Priyambodo Priyambodo Priyambodo Priyambodo Putri, Chicka R.P. Putri, Chicka Refina Rahma Ramadhan, Viki Rustiati, Elly L. Sa'uddah, Lousanja D. Samsul Bakri Sidik, Mahfud Srihanto, Eko A. Sukatmoko Sukatmoko, Sukatmoko Susandi, Waryoko Susanto, Alvin Wiwiet Valinata, Sisca Virnarenata, Elsa Yani, Ani Andri Yanti Yulianti Zulkarnain, Danisworo