Journal of Innovative and Creativity
Vol. 6 No. 1 (2026)

Phytochemical Screening And Antibacterial Activity Of N-Hexane, Ethyl Acetate, And Ethanol Extracts Of Malacca Fruit (Phyllanthus Emblica L.) Against Escherichia Coli And Staphylococcus Aureus

Mariany Razali (Universitas Tjut Nyak Dhien)
Yessi Febriani (Universitas Tjut Nyak Dhien)
Muhammad Taufik (Universitas Sumatera Utara)
Eva Dasopang (Universitas Tjut Nyak Dhien)
Nurmala Sari (Universitas Tjut Nyak Dhien)
Aldy Fardiansyah (Universitas Tjut Nyak Dhien)
Supran Hidayat Sihotang (Universitas Tjut Nyak Dhien)
Rika silvany (Politeknik Teknologi Kimia Industri)



Article Info

Publish Date
22 Feb 2026

Abstract

Phyllanthus emblica L. (Malacca fruit) is a tropical plant traditionally used for its medicinal properties. This study aimed to identify the secondary metabolite content through phytochemical screening and to evaluate the antibacterial activity of n-hexane, ethyl acetate, and ethanol extracts of Malacca fruit against Escherichia coli and Staphylococcus aureus. The simplicia was extracted using graded maceration. Phytochemical screening revealed the presence of flavonoids, terpenoids, steroids, saponins, and tannins. Antibacterial testing was performed using the agar well diffusion method at concentrations of 3.125–100 mg/mL. Results showed that all three extracts exhibited antibacterial activity against both bacteria. The ethanol extract demonstrated the highest inhibition, with zones of 23.78 mm and 21.63 mm against E. coli and S. aureus at 100 mg/mL, respectively. Inhibition zones increased with increasing extract concentration. The ethanol extract exhibited superior antibacterial activity compared to ethyl acetate and n-hexane extracts, likely due to its ability to extract a broader range of polar bioactive compounds.

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Journal Info

Abbrev

joecy

Publisher

Subject

Education Languange, Linguistic, Communication & Media Mathematics Social Sciences Other

Description

Journal of Innovative and Creatifity (JOECY) publishes research articles in the field of education which report empirical research on topics that are significant across educational contexts, in terms of design and findings. The topic could be in curriculum, teaching learning, evaluation, quality ...