Fusarium wilt, caused by Fusarium oxysporum (Fo), is a major disease affecting potato plants (Solanum tuberosum L.) and contributes significantly to yield losses. This study aimed to isolate and identify antagonistic bacteria from the potato rhizosphere capable of inhibiting the growth of F. oxysporum and to assess their antifungal potential through in vitro analysis. Soil samples were collected from the potato-growing region of Kanreapia, Gowa Regency, Indonesia. The antagonistic potential of the bacterial isolates was evaluated using the dual-culture assay. A total of eight bacterial isolates demonstrated significant inhibitory effects against Fo, representing six genera: Clostridium (75.0%), Bacillus (69.3% and 68.9%), Pseudomonas (67.9% and 62.1%), Staphylococcus (64.8%), Neisseria (64.7%), and Serratia (61.5%). Among these, the isolate identified as Clostridium exhibited the highest inhibition rate (75.0%), while Serratia showed the lowest (61.5%). These findings suggest the potential application of rhizobacteria as biological control agents for managing Fusarium wilt in potatoes.
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