Biology, Medicine, & Natural Product Chemistry
Vol 15, No 1 (2026)

Antioxidant Activity Analysis Using DPPH and Total Phenol Methods of Soursop Leaf Extract (Annona muricata L.)

Wati, Dwi Kartika (Unknown)
Amrullah, Kammal Nizam (Unknown)
Mukarommah, Salsabilatul (Unknown)
Sjakoer, Nour Athiroh Abdoes (Unknown)
Faisal, Faisal (Unknown)
Ramadhan, Majida (Unknown)
Nafisa, Nafisa (Unknown)



Article Info

Publish Date
23 Apr 2026

Abstract

Soursop leaves (Annona muricata L.) are known to contain various bioactive compounds with potential antioxidant properties. This study aimed to evaluate the antioxidant activity of soursop leaf extract using the DPPH (2,2-diphenyl-1-picrylhydrazyl) method and to determine its total phenolic content using the Folin–Ciocalteu method. Antioxidant activity was assessed at extract concentrations of 500 and 1000 ppm, with quercetin used as a positive control. The results showed that the soursop leaf extract exhibited very strong antioxidant activity, with an IC50 value of 9,486 ppm. The total phenolic content of the extract was relatively high, ranging from 102,929 to 195,702 mg GAE/g extract. The strong antioxidant activity observed is likely associated with the high content of phenolic compounds, which play a crucial role in scavenging free radicals. These findings suggest that soursop leaf extract has significant potential as a natural antioxidant source for applications in the functional food and pharmaceutical industries.

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Journal Info

Abbrev

BIOMEDICH

Publisher

Subject

Biochemistry, Genetics & Molecular Biology Medicine & Pharmacology Public Health

Description

BIOLOGY, MEDICINE, & NATURAL PRODUCT CHEMISTRY, this journal is published to attract and disseminate innovative and expert findings in the fields of plant, animal, and microorganism secondary metabolite, and also the effect of natural product on biological system as a reference source for ...