<![CDATA[This research evaluates the readiness of the tissue culture laboratory at SMK Negeri 1 Pandak using an observation instrument of laboratory preparedness. This study focused on the laboratory infrasturucture and management system as the primary object of observation in seven aspects. The instrument's validation was conducted by two Expert of Tissue Culture experts to yield Aiken's V validity coefficients for the seven aspects of laboratory readiness observation instrument ranging from 0.585 to 0.661, indicating that the instrument is valid. Observations of the tissue culture laboratory's readiness also revealed an average total readiness percentage of 76.72%, with the infrastructure (84.61%), facilities (85.84%), and administration (85.71%) aspects categorized as "Very Ready". The occupational health and safety (70%), learning process support (80%), and entrepreneurship development (68.42%) aspects are in the "Ready" category. The laboratory dynamics aspect (62.5%) showed a "Less Ready" category, thus requiring special attention. This research confirms the validity of the instrument and provides an overview of the tissue culture laboratory's readiness, and it can be used to enhance the quality and effectiveness of tissue culture laboratories in vocational agriculture schools. In addition, this result contributes as a strategic evaluation model in the management of vocational education laboratories and provides concrete recommendations for the development of entrepreneurship-based science education through the optimization of facilities and standardized management systems]]>
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