Potential Effects of Gandaria (Bouea macrophylla Griff.) Fruit Ethanol Extract on IL-6 Gene Expression in the Brain of Carbon Tetrachloride (CCl₄)-Induced White Rats (Rattus norvegicus L.)

Khoirunnisa Sidabutar (Universitas Islam Negeri Sumatera Utara)
Zahratul Idami (Universitas Islam Negeri Sumatera Utara)
Leni Widiarti (Universitas Islam Negeri Sumatera Utara)
Nurlian Augustin Ningrum (Universitas Islam Negeri Sumatera Utara)

Bioscientist : Jurnal Ilmiah Biologi
Vol. 14 No. 3 (2026): September

This study aimed to evaluate the effect of gandaria fruit ethanol extract on IL-6 expression in the brain tissue of CCl₄-induced rats. A completely randomized experimental design was employed using 20 male white rats (Rattus norvegicus L.), which were assigned to five groups: normal control (KN), positive control (K+), treatment group 1 (P1) receiving 100 mg/kgBW of gandaria fruit ethanol extract, treatment group 2 (P2) receiving 150 mg/kgBW, and treatment group 3 (P3) receiving 200 mg/kgBW. IL-6 gene expression in brain tissue was analyzed using reverse transcription polymerase chain reaction (RT-PCR), followed by band intensity and relative density analyses. The relative density analysis revealed that the greatest reduction in IL-6 gene expression occurred in the P1 treatment group administered 100 mg/kgBW of gandaria fruit ethanol extract, with a fold change value of 2.5. These findings suggest that gandaria fruit ethanol extract may attenuate the inflammatory response induced by CCl₄ exposure through the suppression of IL-6 expression, with the 100 mg/kgBW dose exhibiting the most pronounced reduction based on relative density analysis.