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Jurnal Teknologi Laboratorium
Published by Poltekkes Kemenkes Yogyakarta
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Core Subject : Science, Education,
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Articles 6 Documents
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Search results for , issue "Vol 3 No 2 (2014): 2014 (2)" : 6 Documents clear
Pengaruh Penambahan Bakteri Probiotik yang Dipacu dengan Prebiotik Ubi Jalar Terhadap Penurunan Jumlah Bakteri Shigella dysenteriae Secara In Vitro Rahmi, Safitri Nur; Putra, F.X Saptono; Suyana, Suyana
Jurnal Teknologi Laboratorium Vol 3 No 2 (2014): 2014 (2)
Publisher : POLTEKKES KEMENKES YOGYAKARTA

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Abstract

Bakteri probiotik adalah mikroba yang bersifat menguntungkan dari golongan Bakteri Asam Laktat (BAL). Antara bakteri yang menguntungkan dan patogen akan terjadi kompetisi. Pertumbuhan Bakteri Asam Laktat (BAL) di usus manusia dapat distimulasi dengan cara memberikan substrat- substrat yang dapat dicerna oleh bakteri tersebut sehingga populasinya meningkat dan melawan bakteri patogen. Substrat- substrat yang dapat digunakan oleh BAL untuk menstimulasi pertumbuhannya dikenal dengan nama prebiotik. Tujuan penelitian untuk mengetahui pengaruh penambahan berbagai dosis bakteri probiotik Lactobacillus casei terhadap jumlah penurunan bakteri Shigella dysenteriae. Desain penelitian ini adalah Post Test with Control. Dalam desain penelitian ini terdapat kontrol dan kelompok eksperimen. Penghitungan jumlah Bakteri Shigella dysenteriae dengan menggunakan selektif media Mac Conkey Agar. Data didapatkan dari jumlah bakteri Shigella dysenteriae setelah penambahan probiotik Lactobacillus casei. Pengolahan data menggunakan software pengolah data. Uji statistik yang digunakan adalah One Way Anova dengan taraf signifikan 5%. Hasil penelitian yang telah dilakukan didapatkan rerata jumlah penurunan bakteri Shigella dysenteriae pada media Mac Conkey Agar adalah 48.14 x 105 CFU/ml, 45.76 x 105 CFU/ml, 42.10 x 105 CFU/ml, 35.90 x 105 CFU/ml, 32.76 x 105 CFU/ml. Hasil uji Anova One Way diperoleh nilai signifikansi 0.000 (<0.05). Ada pengaruh bermakna penambahan berbagai dosis bakteri probiotik Lactobacillus casei terhadap penurunan jumlah bakteri Shigella dysenteriae.
Pengaruh Pemberian Rebusan Daun Salam (Eugenia polyantha Wight.) Terhadap Kadar Kolesterol Low Density Lipoprotein (LDL) Serum Tikus Putih (Rattus norvegicus) Hiperkolesterolemia Afrilliani, Dyah Ari; Supriyanta, Bambang; Rahayu, Muji
Jurnal Teknologi Laboratorium Vol 3 No 2 (2014): 2014 (2)
Publisher : POLTEKKES KEMENKES YOGYAKARTA

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Abstract

High levels of LDL cholesterol can lead to heart disease because of clogging and hardening of the arteries. One of the medicinal plants that can reduce levels of LDL cholesterol in the blood is a bay leaf. The content of flavonoids and tannins in the bay leaves serves to lower levels of fat and cholesterol in the blood. This study aims to determine the effect of decoction of bay leaves (Eugenia polyantha Wight.) to the decrease LDL cholesterol level in mice (Rattus norvegicus) with hypercholesterolemia. This study is purely experimental research with Pre and Post Test With Control Group Design, using 25 white rats as experimental animals were divided into five groups, which are the positive control group, three treatment groups and the comparison group. LDL cholesterol levels were measured by the method of Precipitation Reagent For In Vitro Determination of LDL cholesterol with the CHOD-PAP by photometric systems. Data were analyzed descriptively and statistically using One Way ANOVA test and t-test independent sample. Descriptive analysis found the percentage of the decrease of serum LDL cholesterol levels in mice of hypercholesterolemia at doses 0.18 gram/200 gram of weight was 25.96%, at dosess 0.27gram/200 gram of weight was 23:45%, and at doses 0.36 gram/200 gram of weight was 35.94%. Results of One Way ANOVA test showed a significance of 0.000 which means there is the effect of decoction of bay leaves to the decrease of LDL cholesterol levels in mice with hypercholesterolemia. T-test results found that the decoction of bay leaves at doses 0.36 gram/200 gram of weight lowers LDL cholesterol levels were comparable with administration of simvastatin at doses 0.18 mg/200 gram of weight (P > 0.05).The conclusion of this research was the decoction of bay leaves may lower serum LDL cholesterol levels in hypercholesterolemic mice with percentage of 25.96% at doses 0.18 gram/200 gram of weight, 23:45% at doses 0.27 gram/200 gram of weight, and 35.94% at doses 0.36 gram/200 gram of weight. The decoction of bay leaves at doses 0.36 gram/200 gram of weight may lower LDL cholesterol levels which comparable with administration of simvastatin at doses 0.18 mg/200 gram of weight (p > 0.05).
Pengaruh Pemberian Jus Buah Naga Merah (Hylocereus polyrhizus) Terhadap Kadar Trigliserida Tikus Putih (Rattus norvegicus) Hiperlipidemia Rizqi, Afrida Wira Surya; Widada, Subrata Tri; Martsiningsih, M Atik
Jurnal Teknologi Laboratorium Vol 3 No 2 (2014): 2014 (2)
Publisher : POLTEKKES KEMENKES YOGYAKARTA

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Abstract

Cardiovascular disease is a disease that causes the most deaths in the world. One of its main risk factor is triglyceride levels which make the emergence of plaque in coronary artery. Statin as an option drug in reducing triglyceride levels apparently reported to cause myopathy and kidney failure when used in a long term. Natural product like red dragon fruit began to be developed as a safer alternative. The content of various substances such as niacin, vitamin C and fiber in it useful as antihypertriglyceridemia.This study aims to determine the effect of red dragon fruit (Hylocereus polyrhizus) juice to the decrease of triglyceride levels in hyperlipidemic white rats (Rattus norvegicus).This study was an experimental study with pre and post test with control group design. The subjects were 24 male experimental animals (Rattus norvegicus) were divided into 4 groups: one positive control group given simvastatin 0.18 mg/ 200 gram of weight and three groups treated with red dragon fruit juice doses of 3.6; 5.4 and 7.2 gram/ 200 gram of weight. Then, data were analyzed descriptively with One-Way ANOVA test using SPSS 16.0 for Windows.Results with descriptive analysis obtained that mean of positive control’s pre-test triglyceride level was 104.80 mg/ dl and treatment groups I, II and III respectively 108.15 mg/ dl, 106.47 mg/ dl and 107.43 mg/ dl whereas positive control’s post-test triglyceride level was 51.09 mg/ dl and for the treatment groups were 94.64 mg /dl, 71.01 mg/ dl and 58.75 mg/ dl. One Way ANOVA test obtained p <0.05 which indicated the difference between the treatment of various doses of red dragon fruit juice to white rats’ triglyceride levels. Based on that, means there is the effect of various doses of red dragon fruit (Hylocereus polyrhizus) juice to the decrease of triglyceride levels in hyperlipidemic white rats (Rattus norvegicus).
Pengaruh Berbagai Dosis Jus Buah Sirsak (Annona muricata L.) Terhadap Penurunan Kadar Kolesterol Low Density Lipoprotein (LDL) Serum Tikus Putih (Rattus norvegicus) Dislipidemia Tia, Hesti Dwi; Sistiyono, Sistiyono; Hendarta, Narendra Yoga
Jurnal Teknologi Laboratorium Vol 3 No 2 (2014): 2014 (2)
Publisher : POLTEKKES KEMENKES YOGYAKARTA

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Abstract

Consumption of excessive fat in the food that we consume daily can increase cholesterol, LDL cholesterol and triglycerides in the blood that is called dyslipidemia. Diet, exercise and drugs are the effort can be done to lower the lipid levels. The herbs have been widely used as an alternative to lower cholesterol levels because the active substances contained antihiperlipid properties. Soursop has many benefits in curing some diseases. Soursop contains an active ingredient that can lower LDL’s cholesterol levels, including vitamin C, fiber and niacin. Therefore, the researchers wanted to do experiment using soursop as an alternative for lowering LDL’s cholesterol levels in dyslipidemia white rat serum.The purpose of this experiment was to determine the effect of various doses of soursop juice to decrease of LDL’s cholesterol levels in dyslipidemia white rat serum.This experiment used pre-test and post-test with control group design. Soursop juice doses used was at 0,9; 1,8 and 2,7 g / 200 g BW of white rat. The data were processed using descriptive and statistics by the Kolmogorov Smirnov Test, One Way ANOVA, Post Hoct Test and linear regression in SPSS 16.0 for Windows.The results of this experiment was the percentage decrease of LDL cholesterol levels in the white rat serum of various doses soursop juice, which is 0,9 g / 200 g BW of 46,19%, a dose of 1,8 g / 200 g BW of 52,30 % and a dose of 2,7 g / 200 g BW of 61,62%.There was the effect of various doses of soursop juice to decrease LDL’s cholesterol levels in dyslipidemia white rat serum. The greater the dose of soursop juice given, the greater the reduction in LDL’s cholesterol levels in the serum of dyslipidemia white rat.
Efektifitas Pemberian Perasan Bawang Putih (Allium Sativum Linn) Dosis Tunggal Terhadap Jumlah Telur Cacing Gelang (Toxocara Canis) Secara In Vivo Utami, Ratri Risky; Nuryati, Anik; Nuryani, Siti
Jurnal Teknologi Laboratorium Vol 3 No 2 (2014): 2014 (2)
Publisher : POLTEKKES KEMENKES YOGYAKARTA

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Abstract

Background this research is worm disease problematic in the field of health. Treatment for the disease worms that have been used are synthetic drugs that have side effects are not good for health and can lead to dependence. One of the medicinal plants used for deworming is garlic. Chemical constituents in garlic is allicin that is anthelmintics.To prove that it is carried out in vivo study using dogs infected with Toxocara canis worms as test animals.Objective this research to knowing of the effectiveness of various doses of garlic juice with a gift on the first day against Toxocara canis worm egg counts in vivo and the largest decrease in the effectiveness of Toxocara canis worm egg counts after administration of juice garlic at a dose of 0,5; 1 and 1,5 percent weight within 6 days of observation.Methods this research is an experimental study using dogs infected with Toxocara canis nearly the same number of eggs as well as the research design pretest-posttest with control group.Results the largest decrease in the effectiveness of Toxocara canis worm egg counts after administration juice garlic at a dose of 0,5; 1 and 1,5 percent weight within 6 days of observation known to occur on day 3 with a percentage of effectiveness are 48,49%; 67,18%; dan 82,85%. The higher doses of garlic juice, the more effective to decline in the amount of worm eggs Toxocara canis in vivo.Conclusion this research is garlic juice with various doses are not as effective as once treatment in reducing the the amount of eggs bracelet worm (Toxocara canis) in vivo.
Efektivitas Berbagai Konsentrasi Infusa Daun Salam (Eugenia polyantha Wight) terhadap Daya Antibakteri Staphylococcus aureus Secara In Vitro Apriani, Dewi; Amaliwati, Nur; Kurniati, Eni
Jurnal Teknologi Laboratorium Vol 3 No 2 (2014): 2014 (2)
Publisher : POLTEKKES KEMENKES YOGYAKARTA

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Abstract

Staphylococcus aureus is one of the acne-causing bacterias. Chemical compound in the Bay leaves are well-known as an antibacterial agent to inhibit the growth of Staphylococcus aureus. This study aimed to determine the influence of various Bay leaf (Eugenia polyantha Wight) infusing concentration toward the inhibition of Staphylococcus aureus growth. This research is experimental study with post-test and control group design. The sensitivity test methods toward Staphylococcus aureus were divided into five groups of bay leaf infusing concentration of 60%, 70%, 80%, 90%, and 100%. This method are used in 6 times to obtained 30 datas. Then, the datas were analyzed descriptively and analytically by ANOVA using SPSS 16.0 for Windows. The infusion of Bay leaves had inhibition activity against Staphylococcus aureus growth based on ANOVA test which showed a significance value 0.000 <0.05. There was a proof that the infusion of Bay leave have an effect to inhibit the growth of Staphylococcus aureus.

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