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Pengaruh Penambahan Bakteri Probiotik yang Dipacu dengan Prebiotik Ubi Jalar Terhadap Penurunan Jumlah Bakteri Shigella dysenteriae Secara In Vitro Rahmi, Safitri Nur; Putro, Fx Saptono; Suyana, Suyana
Jurnal Teknologi Laboratorium Vol 3 No 1 (2014): 2014 (1)
Publisher : POLTEKKES KEMENKES YOGYAKARTA

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Bakteri probiotik adalah mikroba yang bersifat menguntungkan dari golongan Bakteri Asam Laktat (BAL). Antara bakteri yang menguntungkan dan patogen akan terjadi kompetisi. Pertumbuhan Bakteri Asam Laktat (BAL) di usus manusia dapat distimulasi dengan cara memberikan substrat- substrat yang dapat dicerna oleh bakteri tersebut sehingga populasinya meningkat dan melawan bakteri patogen. Substrat- substrat yang dapat digunakan oleh BAL untuk menstimulasi pertumbuhannya dikenal dengan nama prebiotik. Tujuan penelitian untuk mengetahui pengaruh penambahan berbagai dosis bakteri probiotik Lactobacillus casei terhadap jumlah penurunan bakteri Shigella dysenteriae. Desain penelitian ini adalah Post Test with Control. Dalam desain penelitian ini terdapat kontrol dan kelompok eksperimen. Penghitungan jumlah Bakteri Shigella dysenteriae dengan menggunakan selektif media Mac Conkey Agar. Data didapatkan dari jumlah bakteri Shigella dysenteriae setelah penambahan probiotik Lactobacillus casei. Pengolahan data menggunakan software pengolah data. Uji statistik yang digunakan adalah One Way Anova dengan taraf signifikan 5%. Hasil penelitian yang telah dilakukan didapatkan rerata jumlah penurunan bakteri Shigella dysenteriae pada media Mac Conkey Agar adalah 48.14 x 105 CFU/ml, 45.76 x 105 CFU/ml, 42.10 x 105 CFU/ml, 35.90 x 105 CFU/ml, 32.76 x 105 CFU/ml. Hasil uji Anova One Way diperoleh nilai signifikansi 0.000 (<0.05). Ada pengaruh bermakna penambahan berbagai dosis bakteri probiotik Lactobacillus casei terhadap penurunan jumlah bakteri Shigella dysenteriae

Pengaruh Penambahan Bakteri Probiotik yang Dipacu dengan Prebiotik Ubi Jalar Terhadap Penurunan Jumlah Bakteri Shigella dysenteriae Secara In Vitro Rahmi, Safitri Nur; Putra, F.X Saptono; Suyana, Suyana
Jurnal Teknologi Laboratorium Vol 3 No 2 (2014): 2014 (2)
Publisher : POLTEKKES KEMENKES YOGYAKARTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (110.718 KB)

Bakteri probiotik adalah mikroba yang bersifat menguntungkan dari golongan Bakteri Asam Laktat (BAL). Antara bakteri yang menguntungkan dan patogen akan terjadi kompetisi. Pertumbuhan Bakteri Asam Laktat (BAL) di usus manusia dapat distimulasi dengan cara memberikan substrat- substrat yang dapat dicerna oleh bakteri tersebut sehingga populasinya meningkat dan melawan bakteri patogen. Substrat- substrat yang dapat digunakan oleh BAL untuk menstimulasi pertumbuhannya dikenal dengan nama prebiotik. Tujuan penelitian untuk mengetahui pengaruh penambahan berbagai dosis bakteri probiotik Lactobacillus casei terhadap jumlah penurunan bakteri Shigella dysenteriae. Desain penelitian ini adalah Post Test with Control. Dalam desain penelitian ini terdapat kontrol dan kelompok eksperimen. Penghitungan jumlah Bakteri Shigella dysenteriae dengan menggunakan selektif media Mac Conkey Agar. Data didapatkan dari jumlah bakteri Shigella dysenteriae setelah penambahan probiotik Lactobacillus casei. Pengolahan data menggunakan software pengolah data. Uji statistik yang digunakan adalah One Way Anova dengan taraf signifikan 5%. Hasil penelitian yang telah dilakukan didapatkan rerata jumlah penurunan bakteri Shigella dysenteriae pada media Mac Conkey Agar adalah 48.14 x 105 CFU/ml, 45.76 x 105 CFU/ml, 42.10 x 105 CFU/ml, 35.90 x 105 CFU/ml, 32.76 x 105 CFU/ml. Hasil uji Anova One Way diperoleh nilai signifikansi 0.000 (<0.05). Ada pengaruh bermakna penambahan berbagai dosis bakteri probiotik Lactobacillus casei terhadap penurunan jumlah bakteri Shigella dysenteriae.

PEMANFAATAN TEPUNG BERAS PUTIH (ORYZA SATIVA L.) VARIETAS IR64 SEBAGAI MEDIA ALTERNATIF UNTUK PERTUMBUHAN JAMUR ASPERGILLUS FLAVUS KURNIAWATI, RIMA; RAHMAWATI, ULLYA; SUYANA, SUYANA
Journal of Nursing and Public Health Vol 9 No 2 (2021)
Publisher : UNIVED Press, Universitas Dehasen Bengkulu

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Pendahuluan: Jamur Aspergillus flavus merupakan jenis jamur pathogen. Salah satu media pertumbuhan jamur adalah Potato Dextrose Agar (PDA) yang merupakan media instan pabrikan dalam bentuk siap pakai, harganya mahal dan hanya dapat ditemukan pada tempat tertentu sehingga dibutuhkan media alternatif yang lebih mudah dibuat dan mudah didapat yaitu media tepung beras putih (Oryza sativa L.) varietas Ir64. Media tepung beras putih memiliki komposisi gizi yang dibutuhkan pertumbuhan jamur. Penelitian ini bertujuan untuk Mengetahui Tepung beras putih (Oryza sativa L.) varietas Ir64 dapat digunakan sebagai media alternatif pertumbuhan jamur Aspergillus flavus. Metode: Penelitian ini merupakan penelitian pra-eksperimen dengan desain penelitian Perbandingan Kelompok Statis (Static Group Comparison). Subjek pada penelitian ini adalah jamur Aspergillus flavus dengan objek penelitian tepung beras putih (Oryza sativa L.) varietas Ir64. Hasil dan Pembahasan: Hasil pengukuran diameter koloni jamur Aspergillus flavus pada media tepung beras putih (Oryza sativa L.) varietas Ir64 rerata 77,54 mm, diameter koloni pada media Potato Dextrose Agar (PDA) rerata 79,87 mm, memiliki selisih diameter rearata 2,33 mm. Efektivitas pertumbuhan koloni jamur Aspergillus flavus dibandingkan dengan media Potato Dextrose Agar (PDA) adalah efektif. Kesimpulan: Media tepung beras putih (Oryza sativa L.) varietas Ir64 dapat digunakan sebagai media alternatif pertumbuhan jamur Aspergillus flavus.

PENGARUH MEDIA SOSIAL TERHADAP KEMUNCULAN IDE BUNUH DIRI PADA REMAJA Maharani, Salma Kartika; Hatini, Dyah Estuti Tri; Suyana, Suyana
Jurnal Mahasiswa BK An-Nur : Berbeda, Bermakna, Mulia Vol 10, No 2 (2024)
Publisher : Universitas Islam Kalimantan Muhammad Arsyad Al-Banjari Banjarmasin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31602/jmbkan.v10i2.13730

Suicide is an act of taking one's life consciously and deliberately due to certain factors. Suicidal ideas arise due to mental health disorders and lack of social support. This research aims to reveal the causes of the suicide phenomenon which is said to have become a trend in recent years and the influence of social media on the emergence of suicidal ideas in teenagers. This research uses a literature study research method with a qualitative descriptive approach. The research results reveal that the phenomenon of suicide has increased in recent years. This is analyzed using Durkheim's theory which focuses on the increase in suicide rates and its relationship with the condition of society. Pressure from society and ideal standards make some individuals decide to commit suicide. Individuals who commit suicide must have suicidal ideas/thoughts. One of the factors that influences the emergence of suicide, especially in teenagers, is the intensity of using social media. The tendency of teenagers to use social media actively causes mental health problems. The existing ideal standards also make teenagers feel pressured, triggering feelings of anxiety, stress, and even depression. Teenagers' difficulty in managing emotions and deciding on solutions makes most of them have suicidal thoughts. This research concludes that (1) the term suicide trend emerged due to an increase in suicide cases caused by pressure from society (2) social media has an influence on the emergence of suicidal ideation in teenagers.

CAMPURAN INFUSA SINGKONG (Manihot esculenta), KACANG KEDELAI (Glycine max (L.) Merrill) DAN EKSTRAK RAGI SEBAGAI MEDIA ALTERNATIF PERTUMBUHAN BAKTERI Staphylococcus aureus Martsiningsih, Martha Atik; Astuti, Fardhiasih Dwi; Kasiyati, Menik; Suyana, Suyana; Rahmawati, Ullya; Rahayu, Muji; Aeni, Ajeng Puspa
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 7 No 1 (2024): The Journal of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jmlt.v7i1.19863

The composition of nutrients needed by bacteria varies greatly such as peptone, meat extract, and agar. Nutrient Agar is one of the media used as a bacterial culture that has a composition such as protein and carbohydrates in the form of meat extract and peptone that suits the needs of most bacteria. The carbohydrate content in cassava, protein in soybeans, and nitrogen sources in yeast extract can be combined into alternative media used as bacterial growth media to determine whether the alternative media combination of cassava carbohydrate source infuse, soybean infuse, and yeast extract can be used to grow Staphylococcus aureus bacteria. This research is a pure experimental study with Post Test-Only Control Design research design. The mean growth in the number of colonies of Staphylococcus aureus bacteria on alternative media was 78,25 x Â CFU/ml and NA media was 94.19 x Â CFU/ml with a mean difference of 15.94 x Â CFU/ml or 16.92%. The mean growth diameter of the number of colonies of Staphylococcus aureus bacteria on alternative media was 2.63 x Â CFU/ml and NA media was 2.66 x Â CFU/ml with a mean difference of 0.03 x Â CFU/ml or 1.12%. The percentage of effectiveness of Staphylococcus aureus bacterial growth is 83.07% or moderately effective and the percentage of effectiveness of Staphylococcus aureus bacterial proliferation is 98.87% or effective. Alternative media mixture of cassava infuse (Manihot esculenta crantz), soybean (Glycine max (L.) Merrill) and yeast extract can be used to grow Staphylococcus aureus bacteria.

ALTERNATIVE MEDIA FOR BACTERIAL GROWTH STAPHYLOCOCCUS AUREUS: SWEET POTATO INFUSA (IPOMOEA BATATAS), SOYBEANS (GLYCINE MAX (L) MERRILL) AND YEAST EXTRACT Martsiningsih, Marta Atik; Astuti, Fardhiasih Dwi; Sistiyono, Sistiyono; Suyana, Suyana; Rahmawati, Ullya; Rahayu, Muji; Musyafaah, Novita Siti
Meditory : The Journal of Medical Laboratory Vol 12, No 1 (2024): Meditory, Volume 12 No. 1 Tahun 2024
Publisher : Jurusan Teknologi Laboratorium Medis, Poltekkes Kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33992/meditory.v12i1.3081

Background: Staphylococcus aureus needs nourishment for its growth. The needed nutrients of carbohydrates, proteins, nitrogen, vitamins, and minerals from the media. The media commonly used for bacterial growth is the nutrient medium for high prices. Alternative media from natural local foodstuffs are obtained easily and cheaply instead of instant media. This study aims to identify alternative media mixed with infuse sweet potato (ipomoea batatas), soybeans (glycine Max (l) Merrill), and yeast extract that can be used to grow the staphylococcus aureus bacteria. Methode: this type of research is pure experimental research, and the design used is the posttest-only control design group. Staphylococcus aureus suspension was grown on alternative media and nutrient agar media as a comparison. measurements of colony diameter and number of colonies were compared between alternative media and nutrient agar. Result The results Research has provided an average number of staphylococcus aureus bacteria that grow on alternative media 78.00 x 104 CFU /ml and nutrient media for 94.19 x 104 CFU /ml. Whereas the colony's diameter of alternative media 2.56 mm, and the media nutrient increased by 2.66 mm. Conclusion: Alternative media from a blend of yam, soy infuse, and yeast extract can be used for the growth of the staphylococcus aureus bacteriaKeywords: Staphylococcus aureus; Alternative Media; Potato Infusa; soy infuse; soy infuse

Effect of The Temperature on The Size of Inhibition Zone of the Clindamycin, Levofloxacin, Tetracycline, and Trimethoprim Activity Against Staphylococcus aureus ATCC 25923 Sistiyono, Sistiyono; Martsiningsih, Marta Atik; Kasiyati, Menik; Rahayu, Muji; Pudyastuti, Rita Rena; Suyana, Suyana; Rahmawati, Ully; Rahmad; Faruq, Zulfikar Husni; Setiawan, Budi
JURNAL INFO KESEHATAN Vol 22 No 3 (2024): JURNAL INFO KESEHATAN
Publisher : Research and Community Service Unit, Poltekkes Kemenkes Kupang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31965/infokes.Vol22.Iss3.1353

Antibiotic sensitivity testing is essential for determining bacterial susceptibility to antibiotics. In disc diffusion testing, several technical factors influence the diameter of the inhibition zone, including incubation temperature, which must be carefully controlled to ensure the validity of test results. This study aims to determine the mean, difference, and analyze the diameter of the inhibition zone of the antibiotics, namely Clindamycin, Levofloxacin, Tetracycline, and Trimethoprim against Staphylococcus aureus on Mueller-Hinton agar media with incubation temperatures of 33°C, 34°C, 35°C, 36°C and 37°C for 18 hours. This research is observational, with a cross-sectional design. The primary data is 100 data on the diameter of the antibiotic inhibition zone, obtained by measuring the diameter of the inhibition zone with different incubation temperatures. The selection of antibiotics is based on the mechanism of action of antibiotics inhibiting bacteria, namely the cell wall or cell membrane that surrounds the bacterial cell; the pieces of machinery that make the nucleic acids DNA and RNA, and the machinery that produces proteins (the ribosome and associated proteins) with a range of inhibition zones based on Internal Quality Control CLSI. The data and the repeated measure statistical test will be processed univariately to determine the significance of the difference in the diameter of the formed inhibition zone using the ANOVA test. The measurement of the inhibition zone diameter on the incubation temperature variation showed a significant difference with a p-value of 0.000 for Levofloxacin, Tetracycline, and Trimethoprim, while the p-value of Clindamycin is 0.010. For the other antibiotics, Levofloxacin, Tetracycline, and Trimethoprim antibiotics, the higher the incubation temperature, the average diameter of the inhibition zone is condensed, while for Clindamycin the higher the incubation temperature, the higher the average diameter of the inhibition zone is the same. Incubation temperature and volume affect the diameter of the antibiotic inhibition zone in the disc diffusion method for the antibiotic sensitivity test. It can be concluded that incubation temperature affects the diameter of the antibiotic inhibition zone in disc diffusion tests. It is recommended for future standardized and precise testing conditions to ensure accurate and reliable antibiotic sensitivity results.

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