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Jurnal Teknologi Laboratorium
Published by Poltekkes Kemenkes Yogyakarta
ISSN : -     EISSN : -     DOI : -
Core Subject : Science, Education,
Chemistry Other
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Articles 6 Documents
 1 
Search results for , issue "Vol 5 No 2 (2016): 2016 (2)" : 6 Documents clear
Modification Of Carry-Blair Transport Media For Storage Salmonella typhi Supriatin, Yati; Rahayyu, Muqni
Jurnal Teknologi Laboratorium Vol 5 No 2 (2016): 2016 (2)
Publisher : POLTEKKES KEMENKES YOGYAKARTA

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Abstract

The aim of this study was to determine transport media modification as alternative media to replace Carry Blair. One type of transport media that often use to carry faeces specimens suspected to contain Salmonella typhi is Carry-Blair media. Studies have been conducted experimentally by storing Salmonella typhi on alternative transport media with Peptone composition, disodium Phosphate, Sodium chloride, Calcium chloride, which is made using a semi-solid and Carry-Blair as a control. Three variety of storage was done (0 hour,6 hours,9 hours) at a temperature 4⁰-8⁰C and then Salmonella typhi was inoculated in Salmonella Shigella Agar using spread plate technique incubated during 24 hours at 37⁰C, counted the number of colonies by the plate count method using the colony counter. The results of ANOVA could be concluded that modification media could be use as alternative media replace Carry-Blair at 6 hours. Based on regression correlation test was assumed that the Salmonella typhi bacteria still life at less than 11 hours 54 minutes.
Pemeriksaan Golongan Darah Sistem ABO Metode Slide dengan Reagen Serum Golongan Darah A, B, O Oktari, Anita; Silvia, Nida Daeninur
Jurnal Teknologi Laboratorium Vol 5 No 2 (2016): 2016 (2)
Publisher : POLTEKKES KEMENKES YOGYAKARTA

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Abstract

The standard examination blood type was performed using a reagent that actually less economical. This research in order to determine whether serum blood type can be used as a reagent blood type. It has been already conducted research antibodies in the serum of blood type A, B, and AB. Each serum would reacted with antigent in the blood, indicated by the agglutination. The research method was experimental by performing blood type with drops of serum in the blood type A, B and AB then saw the grade agglutination. The data obtained were processed statistically using Kruskal Wallis. The result showed serum A, B, and O can be used as a substitute reagents alternative anti – A, anti – B, and anti – AB in determining blood type. Based on analysis, quality clotting produced by serum aglutination is not same with the reagent anti – A, anti – B, and anti – AB in determining blood type.
Identifikasi Zat Pewarna Rhodamin B Dan Methanyl Yellow Pada Kerupuk Yang Dijual Di Pasar Beringharjo Yogyakarta Tahun 2016 Rahayu, Muji; Mahmuda, Yulia Irma
Jurnal Teknologi Laboratorium Vol 5 No 2 (2016): 2016 (2)
Publisher : POLTEKKES KEMENKES YOGYAKARTA

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Abstract

“Kerupuk” is one kindof complementary foods preferred by many people. “Kerupuk” can add the taste of the food and can be eaten as a snack. Consumers need to be cautious in consuming “kerupuk”, because there are still many “kerupuk” made with prohibited dyes, especially Rhodamine B and Methanyl yellow. The presence of rhodamine B and methanyl yellow in colored “kerupuk” can cause the color look unnaturally strong. Rhodamine B and methanyl yellow can cause health problems if they are consumed. This research aims to determine the percentage of rhodamine B and methanyl yellow which is used on “kerupuk” sold in Beringharjo Yogyakarta Year 2016. The samples used in this study are 27 samples, which consist of “kerupuk bawang” and “kerupuk taro” and are acquired in Beringharjo Yogyakarta. “kerupuk bawang” and colorful “kerupuk taro” are separated by color, only yellow and red ones are taken. The samples were tested for rhodamine B and methanyl yellow qualitatively using Thin Layered Chromatography (TLC) method. Results of laboratory tests show that 2 of 15 samples tested contain rhodamine B (13.3%) and none of 12 samples contain methanyl yellow
Pemanfaatan Sari Ubi Jalar Ungu (Ipomoea batatas poiret) Sebagai Zat Pewarna Pada Pewarnaan Staphylococcus aureus yuniarty, Tuty; Misbach, Siti Rachmi
Jurnal Teknologi Laboratorium Vol 5 No 2 (2016): 2016 (2)
Publisher : POLTEKKES KEMENKES YOGYAKARTA

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Abstract

The sweet potato purple (Ipomoea batatas poiret) is a type of tubers which have a component of starch, consisted of 30-40% amylase and 60-70% amylopectin. In addition, purple sweet potato also contains a lot of antioxidants derived from anthocyanins, which amounted to 110.51 mg/100 g, phenolic compounds contained in anthocyanin pigments which can stain the cell wall is peptidoglycan of gram-positive bacteria, Staphylococcus aureus. The purpose of this research is to determine whether the purple sweet potato essence can be used as an alternative to the staining of Staphylococcus aureus. This study is a Quasi-experimental design, results are compared directly between gram staining results of Staphylococcus aureus on gram staining using purple sweet potato essence as an experimental group and the gram staining using gentian violet as the control group. The results showed the bacteria with coccus form and reddish violet color presented on gram staining of the experimental group and the control group. The conclusion is the essence of purple sweet potato can be used as an alternative to the staining of Staphylococcus aureus.
Manfaat Prebiotik Tepung Pisang Kepok (Musa paradisiaca formatypica) terhadap Pertumbuhan Probiotik Lactobacillus casei secara In Vitro Hardisari, Ratih; Amaliawati, Nur
Jurnal Teknologi Laboratorium Vol 5 No 2 (2016): 2016 (2)
Publisher : POLTEKKES KEMENKES YOGYAKARTA

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Abstract

This background reviewed cases of diarrhea, one of health problems related to the digestive tract and caused by an imbalance in intestinal microflora. Probiotics are microorganisms that can be used to balance the intestinal microflora’s population. Prebiotics are nutrients required for the growth of good bacteria in human’s intestine. Kepok banana (Musa paradisiaca formatypica) that contains inulin and FOS is a natural source of prebiotics. The purpose of this study is to observe the effect of various concentrations of kepok banana flour (Musa paradisiaca formatypica) on the growth of probiotic bacterium Lactobacillus casei. In order to observe the prebiotic activity of Lactobacillus casei, post-test with control group design method is used toward the kepok banana flour. The amounts of kepok banana flour concentration used are varied, which are 2%, 4%, 6%, 8 %, and 10%. As much as 30 data was resulted from this research that was, later, descriptively analyzed with One-Way ANOVA test using SPSS 16.0 for Windows. The research’s finding shows the average numbers of bacterium Lactobacillus casei (the number of bacteria x 107CFU/ml) produced in the media with the addition of MPF’s concentrations of 2%, 4%, 6%, 8%, and 10% are, respectively, 29187,5 ; 42491,67 ; 52225 ; 89879,17 and 155108,33. This research also obtained a significance value of 0.000 of the One-way Anova test. The conclusion of this study is that there is an in vitro effect of adding various concentrations of kepok banana flour on the growth of probiotic bacterium Lactobacillus casei.
Gambaran Kadar Kolesterol-LDL (Low Density Lipoprotein) Sebelum dan 48 Jam Sesudah Melakukan Satu Kali Terapi Bekam Basah Pada Penderita Hipertensi Dengan Pola lima titik Suryanta, Suryanta; Septiana, Winda
Jurnal Teknologi Laboratorium Vol 5 No 2 (2016): 2016 (2)
Publisher : POLTEKKES KEMENKES YOGYAKARTA

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Abstract

Hypertension, or more commonly known as high blood pressure is a condition in which a person got an increasing blood pressure upper normal, resulting in increasing morbidity and mortality. The long hypertension is one risk factor for cardiovascular disease, which is one cause of atherosclerosis. Atherosclerosis is a very progressive diseases that causes hardening of the arteries due to the blockage by oxidized cholesterol. Atherosclerosis begins with the build up of LDL-cholesterol. There are two handling of LDL-cholesterol; pharmacological and non-pharmacological. Nonpharmacologic is done with wet cupping therapy. The aim of this study is to determine the average LDL-cholesterol levels before and after the wet cupping therapy with five-point pattern. This research is descriptive research, then presented in the form of tables to showing the results of the study. This study was done Talunombo, Sidomulya, Pengasih, Kulon Progo. This research object is venous blood samples taken from hypertensive patients as research subjects. Descriptive test results obtained an average LDL-cholesterol levels before the wet cupping therapy is 114,182 mg/dl and after wet cupping is 115,618 mg/dl. The conclusion of this study is the average LDL-cholesterol levels prior to the wet cupping therapy with a five-point pattern is 114,182 mg/dl and after wet cupping with five-point pattern is 115,618 mg/dl.

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