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Pemanfaatan Sari Ubi Jalar Ungu (Ipomoea batatas poiret) Sebagai Zat Pewarna Pada Pewarnaan Staphylococcus aureus yuniarty, Tuty; Misbach, Siti Rachmi
Jurnal Teknologi Laboratorium Vol 5 No 2 (2016): 2016 (2)
Publisher : POLTEKKES KEMENKES YOGYAKARTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (258.29 KB)

Abstract

The sweet potato purple (Ipomoea batatas poiret) is a type of tubers which have a component of starch, consisted of 30-40% amylase and 60-70% amylopectin. In addition, purple sweet potato also contains a lot of antioxidants derived from anthocyanins, which amounted to 110.51 mg/100 g, phenolic compounds contained in anthocyanin pigments which can stain the cell wall is peptidoglycan of gram-positive bacteria, Staphylococcus aureus. The purpose of this research is to determine whether the purple sweet potato essence can be used as an alternative to the staining of Staphylococcus aureus. This study is a Quasi-experimental design, results are compared directly between gram staining results of Staphylococcus aureus on gram staining using purple sweet potato essence as an experimental group and the gram staining using gentian violet as the control group. The results showed the bacteria with coccus form and reddish violet color presented on gram staining of the experimental group and the control group. The conclusion is the essence of purple sweet potato can be used as an alternative to the staining of Staphylococcus aureus.
PEMBERDAYAAN MASYARAKAT DESA AWILA KECAMATAN MOLAWE DALAM BENTUK PEMERIKSAAN URINE RUTIN SERTA PELATIHAN PEMBUATAN JUS ALBEDO SEMANGKA SEBAGAI AGEN DETOKSIFIKASI GINJAL Orno, Theosobia Grace; Usman, Julianti Isma Sari; Atmaja, Ratih Feraritra Danu; Yuniarty, Tuty; Hasan, Aswiro; Sarita, Sultina
Jurnal Pengabdian Masyarakat Sasambo Vol 5, No 2 (2024): Mei
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jpms.v5i2.1487

Abstract

Kemandirian masyarakat merupakan salah satu tujuan program pemberdayaan masyarakat dalam bidang kesehatan. Desa Awila merupakan salah satu desa di Kecamatan  Molawe Kabupaten Konawe Utara yang memiliki masalah pada kesehatan ginjal. Kegiatan ini bertujuan untuk melakukan pemeriksaan urine rutin sebagai pemeriksaan laboratorium dasar fungsi ginjal, mengedukasi masyarakat tentang pentingnya menjaga kesehatan ginjal serta melatih masyarakat untuk secara mandiri membuat dan mengonsumsi jus albedo semangka yang terbukti secara ilmiah dapat meningkatkan laju filtrasi glomerulus ginjal. Hasil kegiatan pengabdian kepada masyarakat menunjukkan terdapat beberapa parameter pemeriksaan urine rutin yang abnormal diantaranya proteinuria sebanyak 69%, leukosituria dengan persentase 44%, hematuria dan glukosuria berturut-turut sebesar14% dan 6%. Terdapat peningkatan pengetahuan masyarakat pasca edukasi sebesar 92% disertai komitmen masyarakat untuk secara mandiri menerapkan pola konsumsi jus albedo semangka sehari-hari.  
Uji Daya Hambat Sari Daun Alpukat (Persea americana mill) terhadap Pertumubuhan Escherichia coli Yuniarty, Tuty; Hasjim, Lisfaresliana
Health Information : Jurnal Penelitian Digitalisasi Versi Cetak
Publisher : Poltekkes Kemenkes Kendari

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (286.329 KB) | DOI: 10.36990/hijp.v9i2.70

Abstract

Avocado leaves (Percea americana mill) are a part of avocado plants that have benefits as traditional medicines. Avocado leaves are potentially used as anti-diarrhea based on the content of chemicals contained therein, namely saponins, alkaloids, tannins, flavanoids, polyphenols, quercetin which are used to kill pathogenic bacteria, one of which is Escherichia coli. Escherichia coli is an opportunist germ that is commonly found in the human intestine as a normal flora. The purpose of this study was to determine the inhibition of avocado leaf extract on the growth of Escherichia coli bacteria. This type of research is an Experimental laboratory. The research design used in this study was static group comparison because this study was conducted to see differences in concentrations of 10%, 15%, 25%, 50% and 75% of avocado leaf extract in inhibiting the growth of Escherichia coli bacteria by looking at the clear zone formed. The results showed that at a concentration of 25%, 50% and 75% a clear zone (inhibition zone) was formed, whereas at concentrations of 10% and 15% no clear zone (inhibition zone) was formed. From the results of this study it can be concluded that avocado leaf extract can inhibit the growth of Escherichia coli at concentrations of 25%, 50% and 75%.
PEMBERDAYAAN MASYARAKAT SEBAGAI UPAYA PROMOTIF DAN PREVENTIF SINDROM METABOLIK PADA MASYARAKAT MELALUI PROGRAM MINCEU ANTI-MEARO Yuniarty, Tuty; Atmaja, Ratih Feraritra Danu; Orno, Theosobia Grace; Sari, Julianti Isma; Fauzi, Ahmad Zil; Putra, Ahmat Rediansya
Jurnal Abdi Insani Vol 12 No 7 (2025): Jurnal Abdi Insani
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/abdiinsani.v12i7.2629

Abstract

Infectious diseases remain a major public health concern due to their high morbidity and mortality rates, including conditions such as cardiovascular diseases, cancer, chronic respiratory diseases, and diabetes mellitus. Cardiovascular disease and diabetes are strongly associated with central obesity, dyslipidemia, elevated fasting blood glucose, and hypertension. Metabolic syndrome is defined as a constellation of complex metabolic abnormalities that significantly increases the risk of developing cardiovascular disease and type II diabetes mellitus. The key components implicated in the pathogenesis of metabolic syndrome include hypertension, central obesity, insulin resistance, and atherogenic dyslipidemia. This study aimed to educate the community about metabolic syndrome while simultaneously conducting early detection. The screening procedures included measurements of blood pressure, waist circumference, blood glucose levels, the measurement of total cholesterol, triglycerides, and low density lipoprotein (LDL) levels was performed using the Point of Care Testing (POCT) method. A total of 52 respondents participated in this activity, consisting of 57.7% (n=30) females and 42.3% (n=22) males. The results indicated that 92.3% (n=48) had normal blood glucose levels, 100% (n=52) had normal total cholesterol, and 21.2% (n=11) had normal LDL levels. Normal blood pressure was observed in 34.6% (n=18), while 67.3% (n=35) were found to have central obesity. Overall, 9.6% (n=5) of respondents were diagnosed with metabolic syndrome, whereas 90.4% (n=47) were not. Most respondents demonstrated metabolic risk factors such as hypertension, central obesity, and elevated LDL levels, despite having normal blood glucose and total cholesterol. These findings underscore the importance of continuous public health education and targeted interventions to prevent and reduce the prevalence of metabolic syndrome in the community.
Pengaruh Ekstrak Pogostemon cablin Benth terhadap Interleukin-6, Glukosa Darah, Hemoglobin dan Hematokrit pada Tikus Wistar yang Diinduksi Dietilnitrosamin Atmaja, Ratih Feraritra Danu; Yuniarty, Tuty; Misbah, Sitti Rachmi; Malik, Handayani
Jurnal Fitofarmaka Indonesia Vol 11, No 1 (2024): JURNAL FITOFARMAKA INDONESIA
Publisher : Faculty of Pharmacy, Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33096/jffi.v11i1.1092

Abstract

Mortality and morbidity rates of hepatocellular carcinoma continue to rise worldwide. The role of inflammation, regulation of glucose metabolism, and anemia is widely studied concerning the development and life expectancy of patients with hepatocellular carcinoma. In several cancers, efforts to develop Pogostemon cablin benth plant potential drug candidates for hepatocellular carcinoma have been carried out at metabolomic, proteomic, and molecular levels. However, no studies have been found regarding the study of anti-inflammatory effects, metabolism, and anemic conditions of Pogostemon cablin benth intervention in hepatocellular carcinoma. This study is a pre-post experimental in-vivo study aimed at looking at the ability of Pogostemon cablin benth extract against interleukin-6 (IL-6) as an inflammatory marker, blood glucose levels as a marker of metabolic regulation and hemoglobin and hematocrit levels at as markers of anemia in hepatocellular carcinoma. A model of hepatocellular carcinoma was performed in 25 peritoneal-induced diethylnitrosamine-induced Wistar rats. The mice were divided into five groups, namely group 1 (K1), group 2 (K2), group 3 (K3), positive control (KP), and negative control (KN). Pogostemon cablin benth ethanol extract was administered orally to mice K1 40mg/kg body weight; K2:80mg/kg rat body weight; K3:120mg/kg body weight, while the positive control group was given Sorafenib. Interleukin-6 levels were measured using the enzyme-linked immunoassay principle, while glucose, hemoglobin, and hematocrit levels were measured using the enzymatic principle of glucose dehydrogenase-flavin adenine dinucleotide (GDH-FAD). The study showed no difference in blood glucose levels of mice between pre- and post-induction diethylnitrosamine. Induction of diethylnitrosamine obtained significantly led to a significant decrease in hemoglobin levels (15.54 g/dl ±1.28 vs. 14.31 g/dl ±1.11) and hematocrit (45.83% ±3.77 vs 42.20% ±3.25) levels between pre and post-induction. Mice in group 1 (K1) showed a significant increase in blood glucose levels between pre and post-oral Pogostemon cablin benth extract (p<0.05). There was no significant difference in hemoglobin and hematocrit levels in the treatment group between pre-intervention Pogostemon cablin benth extract. However, KN that did not receive Pogostemon cablin benth extract intervention significantly decreased hemoglobin and hematocrit levels (p<0.01). Decreased levels of the pro-inflammatory cytokine IL-6 occurred in K2 (p<0.05) after receiving oral Pogostemon cablin benth extract intervention. Based on this study, Pogostemon cablin benth extract may decrease IL-6 levels and is thought to influence hemoglobin and hematocrit levels in diethylnitrosamine-induced Wistar rats.
Production Of Liquid Composter Using A Household Scale Biogas Reactor In The Perspective Of Toxicological Studies On Public Health Darmayanti, Setya; Yuniarty, Tuty; Kushayati, Nuris; Sari, Mulia Safrida; Kusbandiyah, Jiarti
Jurnal Kesehatan Manarang Vol 9 No 3 (2023): December 2023
Publisher : Politeknik Kesehatan Kemenkes Mamuju

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33490/jkm.v9i3.976

Abstract

Organic waste in the Health Polytechnic Of Kendari has been transported by garbage transport trucks to the Puuwatu TPAS or destroyed by burning. Burning garbage will hurt air quality. This study aims to determine the adequate time needed to produce quality liquid compost from organic waste at the Health Polytechnic Of Kendari and examine the toxicological effects of waste on human health. The results showed that the effective time for producing liquid compost was 36 days with a volume of 2500 mL, and the fastest liquid compost production was 7 days with a volume of 1200 mL. The ideal ratio of C/N composting is 30 : 1. There was a temperature fluctuation within 109 days. The mesophilic phase at the beginning of this study lasted 22 days with a temperature range of 34.5 – 34.9 oC. The second phase is the thermophilic phase, characterized by an increase in temperature, namely up to 40-54.9 oC, which lasts for 36 days. And the maturation stage in this study lasted for 51 days with a temperature range of 44.4 – 32.2 oC. The pH of liquid compost from organic waste has increased, ranging from 2.3 – 4.2. on day 7, the compost turned light brown; from day 44 to day 109, it turned dark brown. Liquid compost production from day 7 to day 85 has a distinctive odour, while liquid compost from day 95 to day 109 has a slight odour. The toxicological effects of organic waste include polluting the environment and disrupting human health, namely respiratory problems, heart problems, cancer, risk of death or premature death.
PEMBERDAYAAN MASYARAKAT DESA AWILA KECAMATAN MOLAWE DALAM BENTUK PEMERIKSAAN URINE RUTIN SERTA PELATIHAN PEMBUATAN JUS ALBEDO SEMANGKA SEBAGAI AGEN DETOKSIFIKASI GINJAL Orno, Theosobia Grace; Usman, Julianti Isma Sari; Atmaja, Ratih Feraritra Danu; Yuniarty, Tuty; Hasan, Aswiro; Sarita, Sultina
Jurnal Pengabdian Masyarakat Sasambo Vol. 5 No. 2 (2024): Mei
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jpms.v5i2.1487

Abstract

Kemandirian masyarakat merupakan salah satu tujuan program pemberdayaan masyarakat dalam bidang kesehatan. Desa Awila merupakan salah satu desa di Kecamatan  Molawe Kabupaten Konawe Utara yang memiliki masalah pada kesehatan ginjal. Kegiatan ini bertujuan untuk melakukan pemeriksaan urine rutin sebagai pemeriksaan laboratorium dasar fungsi ginjal, mengedukasi masyarakat tentang pentingnya menjaga kesehatan ginjal serta melatih masyarakat untuk secara mandiri membuat dan mengonsumsi jus albedo semangka yang terbukti secara ilmiah dapat meningkatkan laju filtrasi glomerulus ginjal. Hasil kegiatan pengabdian kepada masyarakat menunjukkan terdapat beberapa parameter pemeriksaan urine rutin yang abnormal diantaranya proteinuria sebanyak 69%, leukosituria dengan persentase 44%, hematuria dan glukosuria berturut-turut sebesar14% dan 6%. Terdapat peningkatan pengetahuan masyarakat pasca edukasi sebesar 92% disertai komitmen masyarakat untuk secara mandiri menerapkan pola konsumsi jus albedo semangka sehari-hari.  
Pemeriksaan Sedimen Urine Sebagai Upaya Deteksi Dini Kejadian Batu Ginjal Masyarakat Desa Awila Kabupaten Konawe Utara Orno, Theosobia Grace; Fauzi, Ahmad Zil; Usman, Julianti Isma Sari; Atmaja, Ratih Feraritra Danu; Yuniarty, Tuty; Rosanty, Anita
Jurnal Masyarakat Madani Indonesia Vol. 3 No. 2 (2024): Mei
Publisher : Alesha Media Digital

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59025/js.v3i2.209

Abstract

Batu ginjal merupakan salah satu utama masalah kesehatan ginjal di dunia termasuk Indonesia. Penegakan diagnosa batu ginjal secara laboratorium klinis dilakukan melalui pemeriksaan sedimen urine. Kegiatan ini bertujuan untuk deteksi dini kejadian batu ginjal masyarakat desa Awila kecamatan Molawe sesuai dengan keluhan masyarakat yang merujuk  pada gangguan fungsi ginjal. Hasil pemeriksaan sedimen urine menunjukkkan mayoritas masyarakat mengalami batu ginjal dengan variasi jenis batu kalsium oksalat, kalsium fosfat maupun kristal asam urat selain adanya sel epitel abnormal sebagai indikasi infeksi saluran kemih. Intervensi berupa edukasi person to person bagi masing-masing masyarakat sesuai hasil pemeriksaan. Diharapkan agar masyarakat lebih meningkatkan self awareness terhadap kesehatan ginjal. Peran pihak puskesmas diharapkan dapat membantu dalam penegakan diagnosa lebih lanjut disertai terapi sesuai gejala klinis  dan  hasil  pemeriksaan laboratorium medis.
Therapeutic Effect of Topical Ointment Ethanol Extract From Patiwala Leaves (Lantana camara) on Histological Profile of Incision Wounds in Diabetic Rat Models Orno, Theosobia Grace; Rosanty, Anita; Atmaja, Ratih Feraritra Danu; Hasan, Zulfikar Ali; Yuniarty, Tuty; Putra, Ahmat Rediansya
Biology, Medicine, & Natural Product Chemistry Vol 14, No 2 (2025)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2025.142.1091-1097

Abstract

Diabetic wounds are one of the neurovascular complications of diabetes. Hyperglycemia complicates the healing of diabetic wounds, which are susceptible to infection and chronic inflammation. Topical treatment using herbal extracts aims to reduce the side effects of surrounding tissue damage. A topical ointment made from ethanol extract of Patiwala (Lantana camara) leaves, which has been tested for product quality, has antibacterial and anti-inflammatory effects that has been proven to help accelerate the healing of diabetic wounds in rats through parameters such as reduced wound diameter, changes in the number of fibroblast cells, and collagen fiber formation seen in the histological profile during 21 days of therapy with a concentration of 15% (p>0.05) compared to the positive control of 10% betadine.