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Jurnal Teknologi Laboratorium

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Published by Poltekkes Kemenkes Yogyakarta

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Chemistry Other

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Articles 5 Documents

Search results for , issue "Vol 7 No 2 (2018): 2018 (2)" : 5 Documents clear

The Presence of Methanol In Alcoholic Beverages Analyzed Using Qualitative Method Navianti, Diah; Tarmizi, Muhammad Ihsan; Holifah, Sinta Nur
Jurnal Teknologi Laboratorium Vol 7 No 2 (2018): 2018 (2)
Publisher : POLTEKKES KEMENKES YOGYAKARTA

An alcoholic beverage contains ethyl alcohol or ethanol (C2H5OH)that is produced from fermentation or distillation of sugars. In many cases, either producers or consumers add ethanol with denaturated alcohol containing methanol (CH3OH) as an additive. Methanol can cause blindness and induce comas, and it is deadly in high doses. This study aimed at investigating the presence of methanol or methyl alcohol in alcoholic beverages sold in Palembang, Indonesia. Seventeen samples collected from small shops and supermarkets were taken by accidental sampling. A chromotropic acid method was used to examine the presence of methanol. The results showed that there were 18% of the samples was positive, and 82% was negative. Based on alcohol content, the research showed that all (100%) samples of group A were negative; 33% of group B was positive, and 33% of group C was positive. The study indicated that methanol was still present in alcoholic drinks sold in markets. The government should inform the society that denatured alcohol contains methanol and, therefore, should not be feasible to consume.

Antibiotic Resistant and Plasmid Conjugative Study of Salmonella typhi Haji Saeed Akreyi, Waleed; Younis Yousif, Samira; Assafi, Mahde
Jurnal Teknologi Laboratorium Vol 7 No 2 (2018): 2018 (2)
Publisher : POLTEKKES KEMENKES YOGYAKARTA

The emergence of multi-drug resistant (MDR) bacteria has endangered the efficacy of antibiotics treatment of pathogenic bacteria worldwide. The aim of this research was to investigate the incidence of Salmonella enterica serovar Typhi in Duhok city, Iraq. Specimens of blood and stool were recruited from 267 patients. S. Typhi isolates were diagnosed depending on morphology, biochemical and serological tests. S. Typhi isolates were tested for their antibiotic resistance. Multi-drug resistant S. Typhi isolates were conjugated with E. coli HB101. The plasmid profile of transconjugants was investigated. 15/267 (5.6%) S. Typhi isolates were identified. Based on their biochemical tests, S. Typhi isolates were categorized into two biotypes (I, 26.66% and II, 73.33%). Four resistance patterns were observed. The resistant pattern to ampicillin and tetracycline was the higher (46.6%). Conjugation experiment showed that all antibiotic markers were transferred from S. Typhi to E. coli HB101 with a conjugation frequency of (0.38Ã—10-5). 13.3% of the S. Typhi isolates were multi-drug-resistant resistant and had two small plasmids. Transconjugants E. coli acquired the resistance from the multi-drug resistant S. Typhi. Antibiotics treatment of the pathogens could be hindered by the constant rise of multi-drug-resistant. Further studies are needed to study the mobile genetic elements and their contribution to antibiotics resistance.

Pengembangan Prekultur Oxgall sebagai Sampel Klinis untuk Deteksi Salmonella typhi dengan Metode Real-time PCR Gunawan, Annisa Pratiwi; Djuminar, Ai; Ernawati, Ernawati; Chaidir, Lidya
Jurnal Teknologi Laboratorium Vol 7 No 2 (2018): 2018 (2)
Publisher : POLTEKKES KEMENKES YOGYAKARTA

Typhoid fever is a significant public health burden in low-income countries caused by Salmonella enterica serotype typhi (S.typhi). Clinical manifestations of typhoid fever are varied and non-specific, making the diagnosis difficult. Using oxgall for pre-incubation as a selective culture medium before amplification of Real-time PCR (RT-PCR) in wholeblood produces a fast and sensitive diagnostic. The purpose of this study was to know the performance oxgall-precultured Real-time PCR for detection of Salmonella sp.. Prior to the sample process, spike method optimization was performed to find out that the reagents were well used for clinical specimens. In the sample process, blood samples from 30 Widal-positive patients were collected for this study . Venous blood samples from typhoid fever patients were taken on the day of diagnosis; 5 ml for blood culture, and 5 ml for RT-PCR. The bacteria were grown in oxgall 10% (standard microbiological laboratory clinics) and incubated for 6 hours (37Â° C) before bacterial DNA was isolated for RT-PCR detection. The results showed that reagen of RT-PCR is good used for a clinical sample and a blood culture was better than RT-PCR using oxgall (positive blood culture results over 24 hours). This suggests that there is a need for further research on the duration of incubation and oxgall concentrations in RT-PCR and the selection of clinical samples.

Aktivitas Antibakteri Kulit Buah Karika Dieng terhadap Shigella flexneri dan Escherichia coli Novalina, Dhiah; Sugiyarto, Sugiyarto; Susilowati, Ari
Jurnal Teknologi Laboratorium Vol 7 No 2 (2018): 2018 (2)
Publisher : POLTEKKES KEMENKES YOGYAKARTA

Karika is an endemic plant of Dieng Plateau. The leaf of Karika has been studied to have antibacterial activity against bacteria that cause diarrhea, while the peels are removed or used as a mixture of animal feed. This study aims to determine the antibacterial activity of Karika peels against bacteria that cause poisoning, i.e Shigella flexneri, and Escherichia coli. The sample fractionated to obtain the fraction of n-hexane and ethyl acetate. The fractions were tested for their antibacterial activity against Shigella flexneri and Escherichia coli by the cup-plate method. The results showed that the ethyl acetate fraction (ethyl acetate 50%) had the highest inhibition on Shigella flexneri and Escherichia coli compared with another concentration. Based on the study can be concluded that the peels of Karikahas antibacterial activity against Shigella flexneri and Escherichia coli.

Potensi Ekstrak Daun Carica Pubescens Sebagai Alternatif Antidiare Bakteri Vibrio cholerae dan Shigella dysentriae Astuti, Tri Dyah; Hadi, Wahid Syamsul
Jurnal Teknologi Laboratorium Vol 7 No 2 (2018): 2018 (2)
Publisher : POLTEKKES KEMENKES YOGYAKARTA

Acute diarrhea is one of the main causes of morbidity and mortality. A people are starting to choose traditional medicines for alternative therapy. Traditional medicines or herbal medicines are considered safer and do not have side effects such as chemical drugs. The purpose of this study was to determine the anti-diarrhea effect of Carica pubescens leaf extract on the bacteria Vibrio cholerae and Shigella dysentriae. This study was conducted by testing the activity of Vibrio cholerae and Shigella dysentriae bacteria on Carica pubescens leaf extract with a well method, which results can be seen from the formation of inhibitory zones. The data obtained were processed using Two Way ANOVA test statistics. The results showed that the leaves extract of Carica Pubescens concentration of 100% had the best therapeutic effect because it had the greatest inhibitory power on the bacteria Vibrio cholerae and Shigella dysentriae.

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