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Jurnal Teknologi Laboratorium
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Articles 5 Documents
Search results for , issue "Vol 8 No 2 (2019): 2019 (2)" : 5 Documents clear
Effect of electromagnetic field on whole blood, biochemical and hormone level in human YAVAŞ, Mehmet Cihan; AKPOLAT, Veysi; GÖRGÜLÜ, Özkan; KAPLAN, İbrahim
Jurnal Teknologi Laboratorium Vol 8 No 2 (2019): 2019 (2)
Publisher : POLTEKKES KEMENKES YOGYAKARTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2235.252 KB) | DOI: 10.29238/teknolabjournal.v8i2.158

Abstract

The electromagnetic field is increasing in our environment and these exposures cause concern. The aim of our study is to investigate the effects of magnetic fields generated by the hairdryer devices used by women working in the same job on their serum biochemistry, whole blood, and hormone values. The sixteen women working continuously in hairdressing salons were included in the study. Two groups of studies were designed: control (n:8, mean age: 22.25±6.04) and experimental group (n:8, mean age:23,62±6.67). The biochemical (median values of alanine aminotransferase, aspartate aminotransferase, triglycerides, and very-low-density lipoprotein were found high) and hormonal results of the experimental group were compared with the biochemical (cholesterol, low-density lipoprotein, and very high-density lipoprotein had high median values) and hormonal results of the control group and no significant difference was found (p>0.05). When the whole blood parameters were examined, the white blood cells and mean platelet volume results of the experimental group were significant (p<0.05), while there was a meaningless difference between red blood cell, hemoglobin, hematocrit, mean corpuscular volume, red cell distribution width and platelet values (p>0.05). It is evident from the results that occupational exposure to magnetic fields constantly leads to changes in the biochemistry, hormone and whole blood parameters of the female.
Potential of Lactobacillus casei shirota’s strain against the biofilm-forming of Salmonella Spp Fauziah, Annisa Rizka; Bahar, Meiska; Ayu Wulandari, Aprilla
Jurnal Teknologi Laboratorium Vol 8 No 2 (2019): 2019 (2)
Publisher : POLTEKKES KEMENKES YOGYAKARTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2615.691 KB) | DOI: 10.29238/teknolabjournal.v8i2.165

Abstract

Biofilm of Salmonella spp. is formed through the expression of biofilm genes associated with proteins (bapA) regulated by curli synthesis genes (csg) which carry out adhesion, colonization, maturation, and dispersion on the surface of the intestinal epithelium. This study aimed to determine the antibiofilm activity of Lactobacillus casei Shirota’S strain (LcS) as an inhibitor of Salmonella spp. biofilm formation in vitro. This research was a true experimental study using Microtiter Plate 96 wells Biofilm Assay method. The sample used was the suspension of Salmonella spp. The treatment was in the form of adding a LcS suspension with a concentration series of 10-1;10-2; 10-3;10-4; and 10-5. Biofilm measurements were carried out using a microplate reader and obtained quantitative data in the form of Optical Density at a wavelength of 595nm. The results of this study showed that LcS suspension has antibiofilm activity ranging from 10-5 concentrations with a percentage of 36.58% (p<0.05). The results of exometabolism LcS can reduce Salmonella growth. Exopolysaccharide (EPS) and sortase-dependent proteins (SrtA) of LcS form barriers as competitive adhesion in inhibiting pathogenic biofilm formation.
Chymase Level in Dengue Virus Infection With or Without Positive Non-Structural 1(NS1) Kasiyati, Menik; Nugraha, Jusak; Kahar, Hartono
Jurnal Teknologi Laboratorium Vol 8 No 2 (2019): 2019 (2)
Publisher : POLTEKKES KEMENKES YOGYAKARTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1227.342 KB) | DOI: 10.29238/teknolabjournal.v8i2.167

Abstract

Thrombocytopenia, leukopenia, and monocytosis are laboratory parameters in the diagnosis of dengue virus infection. In addition to monocyte cells, mast cells also play a role in the innate immune response, where degranulation of mast cells will occur, which will secretion the active vaso mediator, Chymase. Chymase has a role in increasing vascular permeability resulting in plasma leakage in patients with dengue virus infection to determine the number of platelets, leukocytes, monocytes and chymase levels in patients with dengue infection in the acute phase. The platelet count mean in NS1 (+) was 132,140 cells / mm3 and the platelet count in the NS1 group was (-) 176,000 cells / mm3. The mean leukocytes NS1 (+) showed results of 4,350 cells / mm3 and NS1 (-) 5,250 cells / mm3. The mean monocyte NS1 (+) monocyte count was 8.26%, and NS1 (-) group was 8.76%. There were no significant differences in platelet counts, leukocytes and monocytes between NS1 (+) and NS1 (-) (P value> 0.05). The mean Chymase NS1 (+) 23.48, NS1 (-) 23.05 ng / mL and the control group 1.47ng / mL.
The potency of bay leaves extract (Syzygiumpolyanthum) as anti-inflammation in Rattus norvegicus induced with Complete Freund’s Adjuvant Buana, Dwi Candra; Sudiana, I Ketut; Nugraha, Jusak
Jurnal Teknologi Laboratorium Vol 8 No 2 (2019): 2019 (2)
Publisher : POLTEKKES KEMENKES YOGYAKARTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1954.413 KB) | DOI: 10.29238/teknolabjournal.v8i2.177

Abstract

The using of herbal plants in Indonesia as medicine based on traditional knowledge. One of the herbal plants that believed has medication effect is bay leaves (Syzygiumpolyanthum). Bay leaves contain a flavonoid that can prevent inflammation. The study aims to prove the potency of bay leaves extract to prevent the inflammation, which is indicated by decreasing the paw edema thickness in rat induced with Complete Freund's Adjuvant (CFA). This study used 25 animal models of male rat (Rattus norvegicus) strain Wistar that divided into five groups, one group for a normal group (no treatment), one group for positive control which was injected with CFA sub plantar and given treatment with Na CMC and three test groups which is got three different doses of bay leaves extract peroral and the doses were 100 mg/kg BW, 300 mg/kg BW, 400 mg/kg BW, and injected with CFA into the sub plantar. Bay leaves extract was given one hour per oral before injected with CFA. The measurement of paw edema was done at 24 hours after CFA injection. The result of this study showed that bay leaves extract gave the anti-inflammation effect to the rats which were injected with CFA. It is concluded that bay leaves extract containing flavonoids plays a role in inhibit cytokines proinflammatory synthesis.
Total Lymfosit Count (TLC) with CD4 in HIV/AIDS Patients at Kupang Wuan, Adrianus Ola; Herlinalt Gloria Banunu, Ayorince; Kambuno, Norma Tiku
Jurnal Teknologi Laboratorium Vol 8 No 2 (2019): 2019 (2)
Publisher : POLTEKKES KEMENKES YOGYAKARTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (243.602 KB) | DOI: 10.29238/teknolabjournal.v8i2.189

Abstract

Human Immunodeficiency Virus (HIV) is a retrovirus originating from the retroviridae family of the genus lentivirus that infects and damages cells that have a molecule Cluster of Differentiation 4 (CD4), especially T lymphocytes that have receptors with high affinity for HIV. Total lymphocyte count / TLC has been proposed as an alternative guide to CD4 in limited health facilities. This study aims to determine the correlation between Total Lymphocyte Count (TLC) and CD4 in HIV/AIDS patients in the W.Z. Johannes Kupang hospital. The type of this research was observational analytic with a cross-sectional design. The study was conducted on 121 samples of patients who performed CD4 examination and Total Lymphocytic Count (TLC) in the laboratory of W.Z.Johannes Kupang Hospital. The Spearman correlation test shows a significance value of 0,000 with a Spearman correlation value of 0.799. Based on the results of this study it can be concluded that there is a significant correlation between Total Lymphocyte Count and CD4 and shows the direction of positive correlation with a very strong relationship, where the increase in the number of Total Lymphocyte Count is in line with the increase in CD4 counts.

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